Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M8G39_RS26505 Genome accession   NZ_CP097383
Coordinates   5623083..5624786 (+) Length   567 a.a.
NCBI ID   WP_003116249.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain L00-a     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5613937..5622251 5623083..5624786 flank 832


Gene organization within MGE regions


Location: 5613937..5624786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M8G39_RS26445 (M8G39_26445) - 5613937..5614695 (-) 759 WP_199742101.1 hypothetical protein -
  M8G39_RS26450 (M8G39_26450) - 5614989..5615279 (-) 291 WP_106442995.1 hypothetical protein -
  M8G39_RS26455 (M8G39_26455) - 5615372..5615854 (-) 483 WP_106442994.1 hypothetical protein -
  M8G39_RS26460 (M8G39_26460) - 5615859..5616275 (-) 417 WP_106442993.1 hypothetical protein -
  M8G39_RS26465 (M8G39_26465) - 5616378..5617061 (-) 684 WP_121332250.1 hypothetical protein -
  M8G39_RS26470 (M8G39_26470) - 5617074..5617694 (-) 621 WP_121332251.1 hypothetical protein -
  M8G39_RS26475 (M8G39_26475) - 5618474..5618674 (+) 201 WP_034047630.1 AlpA family phage regulatory protein -
  M8G39_RS26480 (M8G39_26480) - 5618692..5620677 (+) 1986 WP_249913944.1 AAA family ATPase -
  M8G39_RS26485 (M8G39_26485) - 5620689..5621213 (+) 525 WP_121332253.1 hypothetical protein -
  M8G39_RS26495 (M8G39_26495) - 5621553..5622251 (-) 699 WP_223677410.1 hypothetical protein -
  M8G39_RS26500 (M8G39_26500) - 5622315..5622854 (-) 540 WP_033938465.1 pilin -
  M8G39_RS26505 (M8G39_26505) pilB 5623083..5624786 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=689369 M8G39_RS26505 WP_003116249.1 5623083..5624786(+) (pilB) [Pseudomonas aeruginosa strain L00-a]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=689369 M8G39_RS26505 WP_003116249.1 5623083..5624786(+) (pilB) [Pseudomonas aeruginosa strain L00-a]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAAAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.582
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376