Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M0220_RS16100 Genome accession   NZ_CP096973
Coordinates   3536266..3538020 (-) Length   584 a.a.
NCBI ID   WP_030072841.1    Uniprot ID   A0AA46TQ99
Organism   Halomonas qinghailakensis strain ZZQ-149     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3531266..3543020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0220_RS16070 (M0220_16025) - 3531793..3532128 (+) 336 WP_030072830.1 hypothetical protein -
  M0220_RS16075 (M0220_16030) - 3532143..3533015 (-) 873 WP_030072832.1 hypothetical protein -
  M0220_RS16080 (M0220_16035) yacG 3533203..3533436 (-) 234 WP_030072834.1 DNA gyrase inhibitor YacG -
  M0220_RS16085 (M0220_16040) coaE 3533504..3534100 (-) 597 WP_264018214.1 dephospho-CoA kinase -
  M0220_RS16090 (M0220_16045) pilD 3534141..3534986 (-) 846 WP_264018215.1 A24 family peptidase Machinery gene
  M0220_RS16095 (M0220_16050) - 3535028..3536257 (-) 1230 WP_264018216.1 type II secretion system F family protein -
  M0220_RS16100 (M0220_16055) pilB 3536266..3538020 (-) 1755 WP_030072841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0220_RS16105 (M0220_16060) - 3538262..3538669 (+) 408 WP_264018217.1 pilin -
  M0220_RS16110 (M0220_16065) - 3538764..3539153 (+) 390 WP_035536546.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M0220_RS16115 (M0220_16070) - 3539247..3539705 (+) 459 WP_264019238.1 anti-virulence regulator CigR family protein -
  M0220_RS16120 (M0220_16075) - 3539796..3540605 (+) 810 WP_030072848.1 inositol monophosphatase family protein -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63798.16 Da        Isoelectric Point: 6.5683

>NTDB_id=685821 M0220_RS16100 WP_030072841.1 3536266..3538020(-) (pilB) [Halomonas qinghailakensis strain ZZQ-149]
MSHPAPESTLSHATSHGGLRGIAKRLVNHGVLTDAQAAVAEQTAAELEISLLQHVVDSGLVETHAAALAAAWEYGLPVVD
LDAIRINALPPANDYPVKILERLDILPLARHGHRLTVAVPYPATLTQLDELQFATGLSVDGVLAPADQLKSSLTQYLAQN
ERSMLDELENVDDAVSALNIVQLGEDEATFDKAIKDSEDAPVVKFVNKILLDAISRGASDIHFEPYETQYRVRFRIDGML
IEIARPPFALRHRIAARLKIMSRLDISERRLPQDGAIKLQLSRTRSIDFRVNSLPTVFGEKIVLRLLDPASAQLGIEQLG
FSPEQQAAYEHALKKPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGINQVNVLPKIGLNFASALR
AFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVASYNIASSVSLIIAQRLARKLCNLC
KKPANMPAEALRKAGFSQQDIRNGTIYTAVGCKQCTQGYKGRIGIYEVVPITDAMRQLIMRDGNALDIDQQARREGYPSL
YQRCLSRVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=685821 M0220_RS16100 WP_030072841.1 3536266..3538020(-) (pilB) [Halomonas qinghailakensis strain ZZQ-149]
ATGAGCCACCCTGCCCCAGAGTCTACGCTAAGTCATGCGACCTCACATGGTGGTCTACGCGGCATTGCTAAACGACTTGT
GAATCACGGTGTGCTTACCGATGCGCAGGCTGCCGTTGCTGAACAAACGGCGGCAGAACTCGAAATATCGCTACTTCAGC
ATGTCGTGGACAGCGGCTTGGTAGAAACCCATGCTGCTGCGCTGGCCGCCGCCTGGGAGTACGGTTTACCGGTTGTGGAT
CTTGATGCTATTCGTATTAATGCCCTGCCACCCGCCAACGACTATCCCGTTAAAATTTTAGAACGATTAGACATTCTGCC
CCTGGCACGCCACGGGCATCGTCTTACCGTTGCGGTGCCCTACCCTGCAACGCTTACCCAGCTTGATGAGCTGCAGTTTG
CTACCGGGCTAAGTGTGGATGGCGTACTGGCACCCGCTGACCAGCTAAAGAGCTCTCTGACGCAGTACCTAGCGCAAAAT
GAACGCAGCATGCTGGATGAGCTGGAAAACGTTGACGACGCCGTCAGCGCACTCAATATCGTTCAGCTTGGCGAAGATGA
GGCGACATTTGATAAGGCAATAAAAGATAGCGAAGACGCCCCAGTGGTTAAATTCGTTAACAAAATATTGCTTGATGCCA
TCAGCCGTGGCGCGTCCGATATTCACTTTGAGCCCTATGAAACCCAGTACCGGGTGCGCTTTCGTATCGATGGCATGCTA
ATTGAAATTGCGCGCCCGCCTTTCGCGCTGCGGCACCGTATTGCAGCCCGTTTAAAAATCATGTCACGGCTAGATATTTC
CGAACGCCGCCTGCCTCAGGATGGCGCGATTAAGCTACAGCTTTCCCGTACCCGCTCAATTGATTTCCGGGTGAACTCAC
TGCCCACCGTGTTTGGTGAGAAAATAGTATTGCGCCTGCTAGACCCAGCCTCTGCGCAGTTGGGCATCGAACAGCTAGGC
TTTAGCCCTGAACAACAGGCCGCCTATGAGCACGCCCTAAAAAAGCCGCAAGGGATGATTCTGGTAACGGGGCCTACCGG
TAGCGGTAAAACCGTCACGCTTTACACAGGCATTAATATCCTCAACCAAATCGAGCGCAATATTTGTACCGCAGAAGACC
CGGTCGAAATTAAAGTATCGGGCATTAACCAAGTGAACGTTCTTCCTAAAATCGGCCTAAACTTTGCCAGCGCGCTGCGT
GCCTTTTTGCGTCAAGACCCGGATGTCGTGATGGTAGGCGAGATTCGTGACTTAGAAACCGCCGAAATTGCCGTGAAAGC
AGCCCAAACAGGCCACTTGGTGCTATCTACCGTACACACTAACTCAGCTGCTGAAACACTAACCCGTTTAGCCAATATGG
GCGTTGCGTCTTATAACATTGCCAGCTCGGTGAGTTTAATCATTGCCCAGCGCTTAGCGCGTAAGCTATGCAACCTGTGT
AAAAAGCCCGCCAATATGCCAGCGGAAGCACTGCGAAAAGCTGGCTTTAGCCAGCAGGATATTCGTAATGGCACTATTTA
CACGGCAGTTGGCTGCAAACAGTGTACTCAAGGTTATAAAGGGCGTATTGGCATTTACGAAGTAGTGCCAATTACCGATG
CTATGCGCCAGTTGATCATGCGCGATGGCAACGCGCTGGATATTGACCAGCAAGCCCGCCGTGAAGGCTACCCCAGCCTG
TATCAGCGCTGTTTAAGCCGCGTGTTGGATGGCAGCACTAGCCTGGAAGAAGTTAACCGCATTAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(208-475)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-161)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.613

98.288

0.517
  pilB Acinetobacter baylyi ADP1

49.74

98.801

0.491
  pilB Acinetobacter baumannii D1279779

49.647

96.918

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.517

98.116

0.476
  pilB Vibrio campbellii strain DS40M4

48.511

97.774

0.474
  pilB Vibrio cholerae strain A1552

52.437

87.842

0.461
  pilF Neisseria gonorrhoeae MS11

50.105

81.678

0.409