Detailed information NTG0685448

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2I87_RS02290
• Genome accession: NZ_CP096942
• Coordinates: 486215..487363 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY5524
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 481215..492363

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I87_RS02265 (M2I87_02260)	-	482366..482662 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M2I87_RS02270 (M2I87_02265)	-	482659..483252 (-)	594	WP_003084544.1	YggT family protein	-
M2I87_RS02275 (M2I87_02270)	proC	483263..484084 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M2I87_RS02280 (M2I87_02275)	-	484096..484788 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2I87_RS02285 (M2I87_02280)	pilT	485003..486037 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M2I87_RS02290 (M2I87_02285)	pilU	486215..487363 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M2I87_RS02295 (M2I87_02290)	czcD	487371..488270 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M2I87_RS02300 (M2I87_02295)	-	488424..488828 (+)	405	WP_003084560.1	NINE protein	-
M2I87_RS02305 (M2I87_02300)	-	489071..490444 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
M2I87_RS02310 (M2I87_02305)	-	490441..491625 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=685448 M2I87_RS02290 WP_003100959.1 486215..487363(+) (pilU) [Pseudomonas aeruginosa strain NY5524]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=685448 M2I87_RS02290 WP_003100959.1 486215..487363(+) (pilU) [Pseudomonas aeruginosa strain NY5524]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382