Detailed information NTG0685068

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2J78_RS01970
• Genome accession: NZ_CP096912
• Coordinates: 420797..421945 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain NY7770
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 415797..426945

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2J78_RS01945 (M2J78_01945)	-	416948..417244 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M2J78_RS01950 (M2J78_01950)	-	417241..417834 (-)	594	WP_003084544.1	YggT family protein	-
M2J78_RS01955 (M2J78_01955)	proC	417845..418666 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M2J78_RS01960 (M2J78_01960)	-	418678..419370 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2J78_RS01965 (M2J78_01965)	pilT	419585..420619 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M2J78_RS01970 (M2J78_01970)	pilU	420797..421945 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
M2J78_RS01975 (M2J78_01975)	czcD	421953..422852 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
M2J78_RS01980 (M2J78_01980)	-	423007..423411 (+)	405	WP_003084560.1	NINE protein	-
M2J78_RS01985 (M2J78_01985)	-	423654..425027 (+)	1374	WP_004365040.1	pyridoxal-phosphate dependent enzyme	-
M2J78_RS01990 (M2J78_01990)	-	425024..426208 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=685068 M2J78_RS01970 WP_003110016.1 420797..421945(+) (pilU) [Pseudomonas aeruginosa strain NY7770]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=685068 M2J78_RS01970 WP_003110016.1 420797..421945(+) (pilU) [Pseudomonas aeruginosa strain NY7770]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCTGATCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382