Detailed information NTG0681565

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PALA39_RS02000
• Genome accession: NZ_CP109920
• Coordinates: 430107..431255 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain PALA39
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 425107..436255

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PALA39_RS01975	-	426258..426554 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PALA39_RS01980 (PALA39_00395)	-	426551..427144 (-)	594	WP_003084544.1	YggT family protein	-
PALA39_RS01985 (PALA39_00396)	proC	427155..427976 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PALA39_RS01990 (PALA39_00397)	-	427988..428680 (-)	693	WP_023464234.1	YggS family pyridoxal phosphate-dependent enzyme	-
PALA39_RS01995 (PALA39_00398)	pilT	428895..429929 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PALA39_RS02000 (PALA39_00399)	pilU	430107..431255 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PALA39_RS02005 (PALA39_00400)	czcD	431263..432162 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PALA39_RS02010 (PALA39_00401)	-	432316..432720 (+)	405	WP_003084560.1	NINE protein	-
PALA39_RS02015 (PALA39_00402)	-	432963..434336 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PALA39_RS02020 (PALA39_00403)	-	434333..435517 (+)	1185	WP_003110017.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=681565 PALA39_RS02000 WP_003100959.1 430107..431255(+) (pilU) [Pseudomonas aeruginosa strain PALA39]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=681565 PALA39_RS02000 WP_003100959.1 430107..431255(+) (pilU) [Pseudomonas aeruginosa strain PALA39]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382