Detailed information NTG0680576

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: XCQ_RS16475
• Genome accession: NZ_CP096227
• Coordinates: 3811664..3813400 (-)
• Length: 578 a.a.
• NCBI ID: WP_011052125.1
• Uniprot ID: A0AAI7ZH88
• Organism: Xanthomonas citri pv. citri strain CQ13
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3790306..3810822	3811664..3813400	flank	842

Gene organization within MGE regions

Location: 3790306..3813400

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
XCQ_RS16395 (XCQ_16395)	-	3790306..3791473 (+)	1168	Protein_3208	IS3 family transposase	-
XCQ_RS16400 (XCQ_16400)	avrXacE2	3792233..3793303 (-)	1071	WP_011052114.1	type III secretion system effector avirulence protein AvrXacE2	-
XCQ_RS16405 (XCQ_16405)	-	3793388..3794665 (-)	1278	WP_190416505.1	lytic murein transglycosylase	-
XCQ_RS16410 (XCQ_16410)	-	3794798..3797788 (-)	2991	WP_011052967.1	Tn3-like element TnXax1 family transposase	-
XCQ_RS16415 (XCQ_16415)	-	3797796..3799070 (-)	1275	Protein_3212	site-specific integrase	-
XCQ_RS16420 (XCQ_16420)	-	3799263..3800339 (+)	1077	WP_011052118.1	DNA-binding protein	-
XCQ_RS16425 (XCQ_16425)	xopAI	3800473..3801363 (+)	891	WP_011052119.1	type III secretion system effector XopAI	-
XCQ_RS16430 (XCQ_16430)	-	3802694..3802978 (+)	285	WP_016849322.1	hypothetical protein	-
XCQ_RS16435 (XCQ_16435)	-	3803206..3804321 (+)	1116	WP_011052122.1	IS1595 family transposase	-
XCQ_RS16440 (XCQ_16440)	-	3804276..3804791 (-)	516	WP_011052123.1	hypothetical protein	-
XCQ_RS16445 (XCQ_16445)	sucD	3804878..3805753 (-)	876	WP_005921370.1	succinate--CoA ligase subunit alpha	-
XCQ_RS16450 (XCQ_16450)	sucC	3805778..3806947 (-)	1170	WP_005915812.1	ADP-forming succinate--CoA ligase subunit beta	-
XCQ_RS16455 (XCQ_16455)	-	3807179..3808792 (+)	1614	WP_003488599.1	HAMP domain-containing sensor histidine kinase	-
XCQ_RS16460 (XCQ_16460)	pilR	3809117..3810511 (+)	1395	WP_015463522.1	sigma-54 dependent transcriptional regulator	Regulator
XCQ_RS16465 (XCQ_16465)	-	3810752..3810919 (-)	168	WP_015463523.1	hypothetical protein	-
XCQ_RS16470 (XCQ_16470)	pilB	3811051..3811170 (-)	120	WP_005915819.1	hypothetical protein	Machinery gene
XCQ_RS16475 (XCQ_16475)	pilB	3811664..3813400 (-)	1737	WP_011052125.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 578 a.a.
• Molecular weight: 62517.63 Da
• Isoelectric Point: 5.4526

>NTDB_id=680576 XCQ_RS16475 WP_011052125.1 3811664..3813400(-) (pilB) [Xanthomonas citri pv. citri strain CQ13]
MNSVVTANLVGITGIARRLVQDGAVEEAVARSAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALG
DDEEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAANGVTSLAEINRVTKD

Nucleotide

• Length: 1737 bp

>NTDB_id=680576 XCQ_RS16475 WP_011052125.1 3811664..3813400(-) (pilB) [Xanthomonas citri pv. citri strain CQ13]
ATGAATAGCGTCGTGACCGCAAATTTGGTTGGAATAACGGGCATCGCGCGTCGTCTGGTGCAAGACGGTGCGGTCGAAGA
AGCAGTTGCGCGATCTGCGATGGATCAGGCATCTGCGGCAAAGGTTCCTCTGCCGCAATGGTTTGCTGAGAAGAAGTTGG
TCACTGCTTCCCAGCTCGCCGCTGCGAATGCGGTCGAGTTCGGCATGCCACTGTTGGACGTGTCGGCGTTCGACGCCAGC
CAAAACGCGGTCAAGCTGGTAAGTGAGGAGCTACTTCAGAAGCACCAAGTGCTACCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTAGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTTCATACGAACTTGGTAGTCGAGCCCA
TCCTTGTGGATGAAGATCAGATCCGTCGCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCGCTGGGCTCCGCGCTCGGT
GACGACGAGGAGGGCATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTTGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCTTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGGCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTAATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGCTGATG
AAGGCTGCGAATGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-153)

• Type II/IV secretion system protein (T2SSE) (201-468)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.514	98.27	0.555
pilB	Acinetobacter baylyi ADP1	55.81	98.27	0.548
pilB	Legionella pneumophila strain ERS1305867	52.021	98.443	0.512
pilB	Vibrio cholerae strain A1552	49.306	99.654	0.491
pilF	Neisseria gonorrhoeae MS11	49.12	98.27	0.483
pilB	Vibrio parahaemolyticus RIMD 2210633	46.964	96.886	0.455
pilB	Vibrio campbellii strain DS40M4	45.518	98.443	0.448