Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OBG92_RS27715 Genome accession   NZ_CP109657
Coordinates   5887461..5888084 (-) Length   207 a.a.
NCBI ID   WP_003148775.1    Uniprot ID   A0A2R3IMZ4
Organism   Pseudomonas aeruginosa strain Zw26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5882461..5893084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OBG92_RS27695 (OBG92_05453) aroB 5883056..5884162 (-) 1107 WP_012077719.1 3-dehydroquinate synthase -
  OBG92_RS27700 (OBG92_05454) aroK 5884212..5884730 (-) 519 WP_003148769.1 shikimate kinase AroK -
  OBG92_RS27705 (OBG92_05455) pilQ 5884742..5886886 (-) 2145 WP_264315654.1 type 4a pilus secretin PilQ Machinery gene
  OBG92_RS27710 (OBG92_05456) comL/pilP 5886940..5887464 (-) 525 WP_034082521.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  OBG92_RS27715 (OBG92_05457) pilO 5887461..5888084 (-) 624 WP_003148775.1 type 4a pilus biogenesis protein PilO Machinery gene
  OBG92_RS27720 (OBG92_05458) pilN 5888081..5888677 (-) 597 WP_003148777.1 type 4a pilus biogenesis protein PilN Machinery gene
  OBG92_RS27725 (OBG92_05459) pilM 5888677..5889741 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  OBG92_RS27730 (OBG92_05460) - 5889926..5892397 (+) 2472 WP_012077723.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22907.32 Da        Isoelectric Point: 4.6862

>NTDB_id=679724 OBG92_RS27715 WP_003148775.1 5887461..5888084(-) (pilO) [Pseudomonas aeruginosa strain Zw26]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVEPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=679724 OBG92_RS27715 WP_003148775.1 5887461..5888084(-) (pilO) [Pseudomonas aeruginosa strain Zw26]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTGCTTGCGCTGGGCTACAACTTCCACCTGAGCGACA
TGCAGGCGCAGCTGGAGCAGCAGGCCGCGGAGGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCGCAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAACTGCCCAGCGACAC
CGAGGTGCCCGGCCTGCTCGAGGACATCACTCGCACCGGACTGGGCAGCGGGCTGGAGTTCGAGGAGATCAAGCTGCTCC
CCGAGGTCACCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGTGGCTATCACGACCTGGCGACCTTT
GTCAGCGGCGTCTCCAGTCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGAGCCCGGCAGTACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3IMZ4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

42.857

91.304

0.391