Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M0D43_RS16690 Genome accession   NZ_CP096142
Coordinates   3891452..3893188 (+) Length   578 a.a.
NCBI ID   WP_252162276.1    Uniprot ID   A0A9Q9MQQ4
Organism   Xanthomonas prunicola strain CIX249     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3886452..3898188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D43_RS16670 (M0D43_16650) pilA 3887132..3887557 (+) 426 WP_252162273.1 pilin Machinery gene
  M0D43_RS16675 (M0D43_16655) - 3887692..3889281 (+) 1590 WP_260807378.1 phosphoethanolamine transferase -
  M0D43_RS16680 (M0D43_16660) - 3889292..3889921 (+) 630 WP_252165100.1 hypothetical protein -
  M0D43_RS16685 (M0D43_16665) - 3889891..3891417 (+) 1527 WP_313905434.1 hypothetical protein -
  M0D43_RS16690 (M0D43_16670) pilB 3891452..3893188 (+) 1737 WP_252162276.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0D43_RS16695 (M0D43_16675) - 3893256..3893375 (+) 120 WP_260807379.1 pilus assembly protein -
  M0D43_RS16700 (M0D43_16680) pilR 3893897..3895351 (-) 1455 WP_313905435.1 sigma-54 dependent transcriptional regulator Regulator
  M0D43_RS16705 (M0D43_16685) - 3895622..3897235 (-) 1614 WP_260807381.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62785.89 Da        Isoelectric Point: 5.4491

>NTDB_id=679673 M0D43_RS16690 WP_252162276.1 3891452..3893188(+) (pilB) [Xanthomonas prunicola strain CIX249]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKKLVSASQLAAANALEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQANNASFGSSMG
DDDDEMGELDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=679673 M0D43_RS16690 WP_252162276.1 3891452..3893188(+) (pilB) [Xanthomonas prunicola strain CIX249]
ATGAATTCAGTAGCTACCACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACGGCAATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAAGCTGG
TGTCGGCGTCGCAGCTGGCGGCGGCCAATGCCTTGGAATTCGGCATGCCGCTGATGGATGTGTCTGTATTCGACGCTAGC
CAGAACGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACGAACCTGGTGGTCGAGGCTA
TCCTGGTGGACGAGGACCAGATTCGTCGCACCTTGGAGCAGTGGCAGGCCAATAACGCTTCGTTTGGCTCGTCAATGGGG
GACGACGACGACGAAATGGGGGAGCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAAGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGTGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGATTACCGGGTGCGCTTGCGGATCGATGGCCTGCTGAAGAATGTGGCCAAGGCGCCG
GTCAAGCTGAGCCAGCGCATCGCCGCCCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATTCTGGACGGCAGCGCAGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAGACGGTGTCGCT
GTATACGGCCTTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GCGTCAATCAGGTCCAGCAGAACAACAAGCGCGGCATGACGTTTGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACTGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTATCGACGCTGCACACCAACGATGCACCGCAGACCATCGCGCGCTTGATGAACATGGGCATTGCGCCGTACAACATCA
CTTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGTCGGTTGTGCAACAACTGCAAGCGTAAGTCGACCTTGCCC
GAGCATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCTGCCGGTATCGAACTGTACGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAAAGTATCGGCATCCGTGATCTGCGCCAGTCGGCCTTGATG
AAGGCCGCGCATGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.13

99.481

0.548
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilB Vibrio cholerae strain A1552

49.306

99.654

0.491
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

97.578

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.734

91.003

0.362