Detailed information NTG0679652

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OBG92_RS01935
• Genome accession: NZ_CP109657
• Coordinates: 404944..406092 (+)
• Length: 382 a.a.
• NCBI ID: WP_033999672.1
• Uniprot ID: A0A2R3IR84
• Organism: Pseudomonas aeruginosa strain Zw26
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 399944..411092

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OBG92_RS01910 (OBG92_00370)	-	401093..401389 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
OBG92_RS01915 (OBG92_00371)	-	401386..401979 (-)	594	WP_003155373.1	YggT family protein	-
OBG92_RS01920 (OBG92_00372)	proC	401990..402811 (-)	822	WP_034080813.1	pyrroline-5-carboxylate reductase	-
OBG92_RS01925 (OBG92_00373)	-	402823..403515 (-)	693	WP_003155374.1	YggS family pyridoxal phosphate-dependent enzyme	-
OBG92_RS01930 (OBG92_00374)	pilT	403730..404764 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
OBG92_RS01935 (OBG92_00375)	pilU	404944..406092 (+)	1149	WP_033999672.1	type IV pilus ATPase PilU	Machinery gene
OBG92_RS01940 (OBG92_00376)	czcD	406100..406999 (-)	900	WP_058146359.1	CDF family zinc transporter CzcD	-
OBG92_RS01945 (OBG92_00377)	-	407152..407556 (+)	405	WP_003155375.1	NINE protein	-
OBG92_RS01950 (OBG92_00378)	-	407798..409171 (+)	1374	WP_053813250.1	pyridoxal-phosphate dependent enzyme	-
OBG92_RS01955 (OBG92_00379)	-	409168..410352 (+)	1185	WP_034080815.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42536.81 Da
• Isoelectric Point: 6.7790

>NTDB_id=679652 OBG92_RS01935 WP_033999672.1 404944..406092(+) (pilU) [Pseudomonas aeruginosa strain Zw26]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLDELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSMTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=679652 OBG92_RS01935 WP_033999672.1 404944..406092(+) (pilU) [Pseudomonas aeruginosa strain Zw26]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAGAAAGGCGGCTCCGACCTGTTCATCACCGCCGGCGTGCCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACCCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTCGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGGATCGAGACCAACATTCCCACCCTGGA
CGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAATACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGATACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGTTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCGATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAAGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAATGACCTGCGACT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCATTTGTCGAGCATGACCCAGGGCCTGAGCCTGGAGATCACCGACG
ACGACCCGGCCGGTCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A2R3IR84

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.546	93.455	0.613
pilU	Vibrio cholerae strain A1552	60	91.623	0.55
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382