Detailed information NTG0677995

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: L2Y97_RS06080
• Genome accession: NZ_CP095742
• Coordinates: 1373698..1375425 (-)
• Length: 575 a.a.
• NCBI ID: WP_247434305.1
• Uniprot ID: -
• Organism: Luteibacter aegosomatissinici strain 353
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1375576..1393955	1373698..1375425	flank	151

Gene organization within MGE regions

Location: 1373698..1393955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Y97_RS06080 (L2Y97_06080)	pilB	1373698..1375425 (-)	1728	WP_247434305.1	type IV-A pilus assembly ATPase PilB	Machinery gene
L2Y97_RS06085 (L2Y97_06085)	-	1375576..1376430 (+)	855	WP_247434307.1	glycosyltransferase family 2 protein	-
L2Y97_RS06090 (L2Y97_06090)	-	1376423..1377622 (+)	1200	WP_247434310.1	glycosyltransferase	-
L2Y97_RS06095 (L2Y97_06095)	-	1377571..1378497 (-)	927	WP_247434313.1	glycosyltransferase	-
L2Y97_RS06100 (L2Y97_06100)	-	1378566..1379585 (+)	1020	WP_247434315.1	class I SAM-dependent methyltransferase	-
L2Y97_RS06105 (L2Y97_06105)	-	1379582..1380478 (+)	897	WP_247434317.1	glycosyltransferase family 2 protein	-
L2Y97_RS06110 (L2Y97_06110)	-	1380398..1381699 (-)	1302	WP_247434320.1	lipopolysaccharide biosynthesis protein	-
L2Y97_RS06115 (L2Y97_06115)	-	1381696..1383567 (-)	1872	WP_247434323.1	hypothetical protein	-
L2Y97_RS06120 (L2Y97_06120)	-	1383672..1385618 (-)	1947	WP_247434326.1	hypothetical protein	-
L2Y97_RS06125 (L2Y97_06125)	pilA2	1385658..1386098 (-)	441	WP_247434329.1	pilin	Machinery gene
L2Y97_RS06130 (L2Y97_06130)	pilA2	1386226..1386672 (-)	447	WP_247434332.1	pilin	Machinery gene
L2Y97_RS06135 (L2Y97_06135)	-	1386960..1388303 (-)	1344	WP_247434335.1	glycosyltransferase family 39 protein	-
L2Y97_RS06140 (L2Y97_06140)	-	1388414..1390510 (+)	2097	WP_247434338.1	S9 family peptidase	-
L2Y97_RS06145 (L2Y97_06145)	-	1390582..1392861 (+)	2280	WP_247434340.1	hypothetical protein	-
L2Y97_RS06150 (L2Y97_06150)	-	1393431..1393955 (+)	525	WP_247434343.1	hypothetical protein	-

Sequence

Protein

• Length: 575 a.a.
• Molecular weight: 62666.28 Da
• Isoelectric Point: 6.4449

>NTDB_id=677995 L2Y97_RS06080 WP_247434305.1 1373698..1375425(-) (pilB) [Luteibacter aegosomatissinici strain 353]
MATQMHQPTLAGLTGMARRLVTEGVLGEAEVRKAVQDAADKRTSLSAWLVDHHLVDSAKLSQVASAEFGMPLMDIGNMVP
GNMPMDLVTEALITKHQALPLFKRGKRLFVGIADPMQSHALDEIKFHSNHMVEPVLVERGQLRRIIDSALSAANASVPAF
DTGEFDELSLDAGPDDGDGTTGIDANANDDAPVVKFVNKILVDAIKRGASDIHFEPFESVYRVRLRMDGILRIVASAPIK
LGSRFASRLKVMSGLDIAERRVPQDGRIKLNLTKTRAIDFRVSTLPTLFGEKIVLRVLDGSSAKLGIDKLGYEEIQKNLY
LDAIEKPYGMVLVTGPTGSGKTVSLYTALNILNTEGRNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNIISSVTLIIAQRLARRLHDCKREITLPPTA
LLAEGYTQEEIDAGMTIYEAVGCDGCNEGYKGRVGIYQVMPMVEDIQKIVLEGGNAMQIAEVARRAGINDLRASALLKVK
NGVTSLTEINRVTKD

Nucleotide

• Length: 1728 bp

>NTDB_id=677995 L2Y97_RS06080 WP_247434305.1 1373698..1375425(-) (pilB) [Luteibacter aegosomatissinici strain 353]
ATGGCAACACAGATGCACCAGCCCACGCTCGCGGGCCTGACCGGCATGGCCCGACGGCTCGTGACCGAAGGCGTCCTTGG
CGAAGCCGAGGTTCGCAAGGCCGTTCAGGATGCCGCCGACAAGCGCACGTCGCTTTCGGCGTGGCTGGTCGACCACCACC
TGGTCGACAGCGCCAAGCTCTCCCAGGTGGCGTCGGCCGAGTTCGGCATGCCCCTCATGGATATCGGGAACATGGTCCCG
GGTAATATGCCCATGGATCTGGTCACCGAGGCCCTGATCACCAAGCACCAGGCCCTGCCCCTGTTCAAGCGGGGAAAGCG
CCTGTTCGTCGGCATCGCCGACCCCATGCAGTCGCATGCCCTTGATGAAATCAAGTTCCACTCCAACCACATGGTGGAGC
CGGTACTGGTTGAGCGCGGCCAACTCCGCCGCATCATCGATAGCGCGCTTTCCGCGGCGAATGCCAGCGTACCCGCCTTT
GATACCGGCGAGTTCGACGAACTATCGCTGGACGCCGGCCCGGACGATGGCGATGGCACCACGGGCATCGATGCGAATGC
GAACGACGATGCGCCCGTCGTCAAGTTCGTCAACAAGATCCTGGTGGATGCGATCAAGCGCGGCGCGTCCGATATCCATT
TCGAACCGTTCGAATCCGTTTACCGCGTGCGCCTGCGCATGGACGGCATCCTCAGGATCGTCGCCTCGGCCCCTATCAAG
CTGGGTAGCCGCTTTGCTTCCCGCCTGAAGGTAATGAGCGGCCTGGATATCGCCGAACGCCGGGTGCCGCAGGATGGCCG
CATCAAGCTCAACCTGACCAAGACCCGCGCTATCGATTTCCGCGTCAGCACCCTCCCTACGCTGTTCGGTGAGAAGATCG
TGCTCCGCGTGCTCGATGGGTCCTCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGGAAATCCAGAAAAACCTCTAC
CTCGATGCCATCGAAAAGCCTTACGGCATGGTGCTCGTCACCGGCCCCACCGGCTCAGGCAAAACCGTATCGCTGTATAC
GGCGCTGAATATCCTGAATACGGAAGGGCGCAACATTTCGACGGTAGAGGACCCGGTCGAAATCCGCGTCGAGGGTATCA
ACCAGGTACAGCAGAACGTCAAGCGCGGCATGACCTTCGCCGCAGCGCTACGCTCGTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGTGACCTTGAAACCGCGGAGATCGCGGTAAAGGCCGCCCAGACCGGTCACATGGTGCTTTC
CACCCTGCACACCAACGATGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGTATCGCGCCCTACAACATCATCTCGT
CGGTGACCCTGATCATTGCCCAGCGCCTGGCCCGCCGCCTGCACGACTGCAAGCGCGAGATCACCCTTCCGCCTACCGCC
CTGCTCGCCGAGGGCTACACGCAGGAGGAGATCGACGCGGGCATGACGATCTACGAGGCCGTGGGTTGCGATGGCTGCAA
CGAGGGCTACAAGGGGCGCGTCGGCATCTACCAGGTCATGCCGATGGTCGAGGATATCCAGAAGATCGTGCTTGAGGGCG
GAAACGCCATGCAAATCGCGGAGGTCGCCCGCCGGGCGGGTATCAACGATCTTCGCGCCTCGGCACTGCTCAAGGTGAAG
AACGGCGTCACCAGCCTGACCGAAATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-150)

• Type II/IV secretion system protein (T2SSE) (199-466)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	54.561	99.13	0.541
pilB	Acinetobacter baylyi ADP1	54.159	98.261	0.532
pilB	Legionella pneumophila strain ERS1305867	50.615	98.957	0.501
pilB	Vibrio cholerae strain A1552	48.577	97.739	0.475
pilF	Neisseria gonorrhoeae MS11	47.257	98.261	0.464
pilB	Vibrio parahaemolyticus RIMD 2210633	46.714	97.913	0.457
pilB	Vibrio campbellii strain DS40M4	46.466	98.435	0.457
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	36.256	100	0.374