Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MUU77_RS04410 Genome accession   NZ_CP095187
Coordinates   964728..966470 (-) Length   580 a.a.
NCBI ID   WP_245092015.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. F37     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 960408..975976 964728..966470 within 0


Gene organization within MGE regions


Location: 960408..975976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUU77_RS04390 (MUU77_04390) - 960801..961226 (+) 426 WP_245092010.1 transposase -
  MUU77_RS04395 (MUU77_04395) coaE 961650..962267 (-) 618 WP_245092012.1 dephospho-CoA kinase -
  MUU77_RS04400 (MUU77_04400) pilD 962301..963164 (-) 864 WP_187573334.1 A24 family peptidase Machinery gene
  MUU77_RS04405 (MUU77_04405) pilC 963174..964430 (-) 1257 WP_245094214.1 type II secretion system F family protein Machinery gene
  MUU77_RS04410 (MUU77_04410) pilB 964728..966470 (-) 1743 WP_245092015.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MUU77_RS04415 (MUU77_04415) - 966685..967533 (+) 849 WP_245092019.1 glycosyltransferase family 2 protein -
  MUU77_RS04420 (MUU77_04420) - 967548..968777 (+) 1230 WP_245092023.1 glycosyltransferase family 4 protein -
  MUU77_RS04425 (MUU77_04425) - 968817..970007 (+) 1191 WP_245092025.1 oligosaccharide flippase family protein -
  MUU77_RS04430 (MUU77_04430) - 969988..971118 (-) 1131 WP_245092027.1 glycosyltransferase -
  MUU77_RS04435 (MUU77_04435) - 971125..971994 (-) 870 WP_245092029.1 FkbM family methyltransferase -
  MUU77_RS04440 (MUU77_04440) - 971991..972776 (-) 786 WP_245092031.1 class I SAM-dependent methyltransferase -
  MUU77_RS04445 (MUU77_04445) - 972879..973973 (+) 1095 WP_245092033.1 glycosyltransferase family 4 protein -
  MUU77_RS04450 (MUU77_04450) - 974030..975964 (-) 1935 WP_245092035.1 hypothetical protein -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62868.29 Da        Isoelectric Point: 6.2649

>NTDB_id=676032 MUU77_RS04410 WP_245092015.1 964728..966470(-) (pilB) [Pseudoxanthomonas sp. F37]
MNAVTSPTANLVGITGIARRLVQDGAMDETTARSAMANATEAKVPLAQWVAEKRLVAPAHLAAANAIEFGIPLIDVTAFD
PVHHAIKLVSEELIQKHQVLPLFKRGGKLFVGTSNPTQTAALTEIQFHTNLVVEPVLVDEDQLRRTLDQWLNAASNLSGA
FGGDDDEGLDNLEVGTDEDMGAGSDSAVDAKGDDTPVVKFVNKVLVDAIKRGASDIHFEPYETDYRVRLRIDGILKQVAK
APVKLSSRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPDQ
QKLFEEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDEVRNISTVEDPVEIRLPGVNQVQQNTKRGMTFAAALRSFLRQ
DPDIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCPKCKKPVE
LPKPALLAEGFTQAEIDAGFTLYDAVGCDECTEGYKGRTGIYQVMPMSDDIQAIVLEGGNAMQIAAVAQKAGIRDLRQSA
LMKVRNGVTSLAEINRVTKD

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=676032 MUU77_RS04410 WP_245092015.1 964728..966470(-) (pilB) [Pseudoxanthomonas sp. F37]
ATGAACGCCGTCACCTCCCCTACCGCCAATCTCGTCGGCATCACCGGCATCGCCCGCCGCCTGGTCCAGGACGGCGCGAT
GGATGAAACCACCGCCCGCAGCGCGATGGCCAACGCCACCGAGGCGAAGGTACCCCTGGCGCAGTGGGTGGCCGAGAAGC
GGCTGGTGGCCCCGGCCCACCTGGCCGCCGCCAACGCCATCGAGTTCGGCATACCGCTGATCGATGTCACCGCCTTCGAC
CCCGTCCACCACGCCATCAAGCTGGTCAGCGAGGAGCTGATCCAGAAGCACCAGGTGCTGCCCCTGTTCAAGCGCGGCGG
CAAGCTGTTCGTCGGCACCAGCAACCCCACCCAGACCGCGGCCCTGACCGAGATCCAGTTCCACACCAACCTGGTGGTGG
AGCCCGTGCTGGTGGATGAGGACCAACTGCGCCGCACCCTGGACCAGTGGCTCAACGCCGCCAGCAACCTGTCCGGCGCC
TTCGGCGGCGACGACGACGAGGGCCTGGACAACCTGGAGGTGGGCACCGACGAGGACATGGGTGCCGGTAGCGACAGCGC
CGTCGACGCCAAGGGCGACGACACCCCGGTGGTCAAGTTCGTCAACAAAGTGCTGGTGGACGCCATCAAACGTGGCGCCT
CGGACATCCACTTCGAGCCCTACGAGACCGACTACCGGGTGCGCCTGCGCATCGACGGCATTCTCAAGCAGGTTGCCAAG
GCGCCGGTGAAGCTGAGCTCGCGCATCGCCGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCC
GCAGGACGGGCGCATCAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCAACGCTGTTCG
GCGAGAAGATCGTGCTGCGCATCCTGGACGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGACCAG
CAGAAACTGTTCGAAGAGGCCATCCATAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGT
GTCGCTGTACACCGCGCTGGGCATCCTGAACGACGAGGTGCGCAACATCTCCACCGTCGAGGATCCGGTGGAAATCCGCC
TGCCCGGCGTGAACCAGGTGCAGCAGAACACCAAGCGCGGCATGACCTTCGCGGCGGCGCTGCGCAGCTTCCTGCGCCAG
GACCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCA
CATGGTGCTGTCCACCCTGCACACCAACGACGCCCCCCAGACCATCGCGCGCCTGATGAACATGGGCATCGCCCCGTACA
ACATCACCAGCTCGGTGACCCTGGTGATCGCCCAGCGCCTGGCGCGGCGGCTGTGCCCCAAGTGCAAGAAGCCGGTCGAG
CTGCCCAAGCCCGCCCTGCTGGCCGAAGGCTTCACCCAGGCCGAGATCGACGCCGGCTTCACCCTGTACGACGCGGTCGG
CTGCGACGAGTGCACCGAGGGCTACAAGGGCCGGACCGGCATCTACCAGGTCATGCCCATGTCCGACGACATCCAGGCCA
TCGTGCTGGAAGGCGGCAACGCCATGCAGATTGCCGCCGTGGCCCAGAAGGCCGGCATCCGCGATCTGCGCCAGTCTGCG
CTGATGAAGGTCCGCAACGGCGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-151)

Type II/IV secretion system protein (T2SSE)

(203-470)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.162

97.931

0.55
  pilB Acinetobacter baylyi ADP1

54.733

100

0.548
  pilB Legionella pneumophila strain ERS1305867

51.494

98.103

0.505
  pilF Neisseria gonorrhoeae MS11

49.649

98.276

0.488
  pilB Vibrio cholerae strain A1552

52.039

88.793

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

46.032

97.759

0.45
  pilB Vibrio campbellii strain DS40M4

45.455

98.621

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.409

100

0.374