Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LEN_RS05235 Genome accession   NZ_AP014940
Coordinates   1225842..1227569 (-) Length   575 a.a.
NCBI ID   WP_096376940.1    Uniprot ID   A0AAU9AL61
Organism   Lysobacter enzymogenes strain M497-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1220842..1232569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LEN_RS05215 (LEN_1073) - 1221799..1222866 (+) 1068 WP_096376936.1 Nudix family hydrolase -
  LEN_RS05220 (LEN_1074) coaE 1223049..1223663 (-) 615 WP_096376937.1 dephospho-CoA kinase -
  LEN_RS05225 (LEN_1075) - 1223663..1224550 (-) 888 WP_096376938.1 A24 family peptidase -
  LEN_RS05230 (LEN_1076) pilC 1224570..1225838 (-) 1269 WP_096376939.1 type II secretion system F family protein Machinery gene
  LEN_RS05235 (LEN_1077) pilB 1225842..1227569 (-) 1728 WP_096376940.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LEN_RS05240 (LEN_1078) - 1227704..1228567 (+) 864 WP_096376941.1 glycosyltransferase family 2 protein -
  LEN_RS05245 (LEN_1079) - 1228564..1229268 (+) 705 WP_096376942.1 class I SAM-dependent methyltransferase -
  LEN_RS05250 (LEN_1080) - 1229265..1230416 (+) 1152 WP_096376943.1 glycosyltransferase -
  LEN_RS05255 (LEN_1081) - 1230413..1231267 (+) 855 WP_172437146.1 GNAT family N-acetyltransferase -
  LEN_RS05260 (LEN_1082) - 1231247..1232308 (-) 1062 WP_172437147.1 NAD(P)-binding domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62529.69 Da        Isoelectric Point: 5.9236

>NTDB_id=67449 LEN_RS05235 WP_096376940.1 1225842..1227569(-) (pilB) [Lysobacter enzymogenes strain M497-1]
MSTATANLVGITGIARRLVMDGALDEVKAREAMAAASAERKPLASYLAENRLATSAQLAAANSIEFGVPLFDASAMDPTQ
SASRLISEELVRKHNALPLFKRGNKLFIGLADPTNSRALDEIKFQTNLVIEAILVDEDLIKSTLDRWMDTNDTLSDATGD
GEGLDTLEVGKDDEGLSAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQVAKVPNK
LHSRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDDQRDLF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDDQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEVAAGITLFEPVGCPDCTEGYKGRTGIYQVMPMTEEIQAIVLEGGNAMQIAAAATRSGVRDLRRSALVKAL
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=67449 LEN_RS05235 WP_096376940.1 1225842..1227569(-) (pilB) [Lysobacter enzymogenes strain M497-1]
ATGTCCACCGCTACTGCCAACCTGGTGGGAATCACCGGCATTGCCCGGCGTTTGGTCATGGATGGCGCCCTGGACGAGGT
CAAGGCCCGCGAAGCCATGGCCGCGGCCAGCGCCGAGCGCAAGCCGCTGGCGTCATACCTGGCTGAGAACCGCCTGGCCA
CCTCGGCCCAGCTCGCCGCCGCCAACTCCATCGAGTTCGGCGTGCCGCTCTTCGACGCCTCGGCGATGGACCCGACCCAG
TCGGCCTCGCGCCTGATCAGCGAAGAACTGGTCCGCAAGCACAACGCCCTGCCCCTGTTCAAGCGCGGCAACAAGCTGTT
CATCGGCCTGGCCGACCCGACCAACAGCCGCGCGCTCGACGAGATCAAGTTCCAGACCAACCTGGTGATCGAGGCGATCC
TGGTCGACGAGGACCTGATCAAGTCCACGCTCGACCGCTGGATGGACACCAACGACACCCTTAGCGACGCCACCGGCGAC
GGCGAGGGCCTGGACACCCTGGAAGTCGGCAAGGACGACGAGGGCCTGTCCGCCGGCGGCGACAGCGGCGTCGACGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAAACCGAATACCGCGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCCAACAAG
CTGCACTCGCGCATCGCCGCACGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCTCGGCCGCGCGGCTGGGCATCGAGAAGCTCGGCTACGAGGACGATCAGCGCGATCTGTTC
GTCAACGCCGTGGTCAAGCCCTACGGCATGGTCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGATCAGCGCAATATCTCCACCGTCGAAGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGTCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGATCTCGAAACCGCCGAAATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGACGCCCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCCT
CGGTGACCTTGGTGATCGCCCAGCGCCTGGCCCGGCGCCTGCACGACTGCAAGCGCGAGGTTCATCTGCCCGAGCACGCG
CTGCTGGCCGAAGGTTTCACCCACGAAGAAGTCGCCGCCGGCATCACCCTGTTCGAACCGGTCGGCTGCCCCGACTGCAC
CGAGGGGTATAAGGGCCGTACCGGCATCTACCAGGTCATGCCGATGACCGAAGAAATCCAGGCGATCGTGCTCGAAGGCG
GCAACGCGATGCAGATCGCCGCCGCCGCCACGCGGTCGGGCGTGCGCGATCTGCGCCGCTCGGCCCTGGTCAAGGCGCTC
AACGGCGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.674

98.609

0.539
  pilB Acinetobacter baumannii D1279779

54.321

98.609

0.536
  pilB Legionella pneumophila strain ERS1305867

50.175

99.478

0.499
  pilF Neisseria gonorrhoeae MS11

46.06

99.304

0.457
  pilB Vibrio cholerae strain A1552

50.874

89.565

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

48.762

91.304

0.445
  pilB Vibrio campbellii strain DS40M4

50.511

85.043

0.43

Multiple sequence alignment