Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MT343_RS18025 Genome accession   NZ_CP094879
Coordinates   4004175..4005290 (-) Length   371 a.a.
NCBI ID   WP_145467872.1    Uniprot ID   -
Organism   Thalassolituus sp. C2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3999175..4010290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MT343_RS18010 - 4001179..4001967 (-) 789 WP_225691082.1 ABC transporter permease -
  MT343_RS18015 - 4001960..4002745 (-) 786 WP_145467876.1 ABC transporter ATP-binding protein -
  MT343_RS18020 - 4002747..4003754 (-) 1008 WP_145467874.1 ABC transporter substrate-binding protein -
  MT343_RS18025 pilU 4004175..4005290 (-) 1116 WP_145467872.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MT343_RS18030 pilT 4005302..4006336 (-) 1035 WP_145467870.1 type IV pilus twitching motility protein PilT Machinery gene
  MT343_RS18035 - 4006464..4007165 (+) 702 WP_145467868.1 YggS family pyridoxal phosphate-dependent enzyme -
  MT343_RS18040 proC 4007271..4008092 (+) 822 WP_145467866.1 pyrroline-5-carboxylate reductase -
  MT343_RS18045 - 4008230..4008817 (+) 588 WP_145467864.1 YggT family protein -
  MT343_RS18050 - 4008814..4009125 (+) 312 WP_145467862.1 DUF167 family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41076.09 Da        Isoelectric Point: 6.3718

>NTDB_id=673863 MT343_RS18025 WP_145467872.1 4004175..4005290(-) (pilU) [Thalassolituus sp. C2-1]
MAMDKFLRLMVEKGASDLFITAGVPPSMKVNGKIMPLSKNPLSAEQTREVVLGLMNSKQQDEFEEKKELNFAINASGIGR
FRASAFYQRNVSGMVLRRIETRIPSVDQLGLPDIIKEISMAKRGLILFVGATGAGKSTSLASMIGHRNKNSKGHILSIED
PIEFIHQHDGCIITQREVGLDTESFDTALKNCLRQAPDVIMIGEVRSAETMEHAITFAETGHLCLATLHANNANQALERV
IHFFPPDRHNQVWMDLSLNLRAIVAQQLIATPDGRSRRAVVEVLLNTPLASDLIRKGEVHELKPLMAKSNEAGMQTYDQA
LYALYEQGEITYEDAIAHADSPNDLRLMIKLGSEADADHLDGATRNLRLQD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=673863 MT343_RS18025 WP_145467872.1 4004175..4005290(-) (pilU) [Thalassolituus sp. C2-1]
ATGGCGATGGATAAGTTTTTACGCCTGATGGTGGAAAAAGGCGCCTCGGATCTGTTTATTACAGCCGGGGTTCCGCCTTC
CATGAAGGTAAACGGTAAAATTATGCCGTTAAGCAAAAACCCGCTCAGTGCCGAGCAAACGCGCGAAGTGGTACTGGGGC
TGATGAACAGCAAACAGCAGGACGAATTTGAGGAGAAAAAAGAACTCAACTTCGCCATTAATGCCAGTGGTATCGGACGT
TTCCGTGCCAGTGCTTTTTATCAGCGTAACGTTTCCGGTATGGTGCTGCGCCGTATCGAAACCCGTATTCCGAGTGTCGA
TCAGCTCGGCCTGCCGGATATTATTAAAGAAATATCCATGGCCAAGCGCGGCCTGATTCTGTTTGTCGGTGCCACCGGTG
CCGGTAAGTCGACCTCGCTGGCGTCGATGATTGGTCACCGTAATAAAAACAGTAAGGGCCATATTCTCAGCATCGAAGAC
CCGATCGAATTTATTCACCAGCACGATGGCTGCATTATTACCCAGCGTGAAGTGGGCTTAGATACCGAGAGTTTTGATAC
CGCCCTGAAAAACTGTCTGCGTCAGGCGCCGGATGTGATCATGATTGGTGAGGTGCGTTCTGCCGAAACCATGGAGCACG
CCATTACCTTCGCCGAAACCGGTCACCTGTGTCTGGCGACCCTGCACGCTAACAATGCTAACCAGGCGCTGGAACGTGTG
ATTCACTTTTTCCCGCCGGACCGTCATAACCAGGTGTGGATGGATTTATCCCTTAACCTGCGCGCTATTGTGGCCCAGCA
GTTAATTGCCACACCGGATGGCCGTTCACGCCGCGCGGTGGTGGAGGTTCTGCTGAATACACCACTGGCGTCGGACCTGA
TCCGTAAAGGTGAGGTGCACGAACTGAAGCCATTAATGGCCAAGTCCAACGAAGCGGGTATGCAGACTTACGACCAGGCG
CTGTATGCACTCTACGAGCAGGGTGAAATCACCTATGAAGACGCCATTGCTCACGCCGACTCGCCAAACGATCTGCGTCT
GATGATTAAACTCGGCTCCGAAGCCGACGCCGATCATCTGGATGGTGCGACACGTAATCTGCGGCTGCAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

73.784

99.73

0.736
  pilU Acinetobacter baylyi ADP1

63.866

96.226

0.615
  pilU Vibrio cholerae strain A1552

53.117

99.461

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.011

96.765

0.426