Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MT343_RS01870 Genome accession   NZ_CP094879
Coordinates   433285..434382 (+) Length   365 a.a.
NCBI ID   WP_366516530.1    Uniprot ID   -
Organism   Thalassolituus sp. C2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428285..439382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MT343_RS01860 - 429120..430523 (+) 1404 WP_145470729.1 FAD-binding oxidoreductase -
  MT343_RS01865 - 430611..433079 (-) 2469 WP_243989451.1 penicillin-binding protein 1A -
  MT343_RS01870 pilM 433285..434382 (+) 1098 WP_366516530.1 pilus assembly protein PilM Machinery gene
  MT343_RS01875 - 434382..434930 (+) 549 WP_145470732.1 PilN domain-containing protein -
  MT343_RS01880 - 434935..435579 (+) 645 WP_145470734.1 type 4a pilus biogenesis protein PilO -
  MT343_RS01885 - 435581..436120 (+) 540 WP_145470736.1 pilus assembly protein PilP -
  MT343_RS01890 pilQ 436176..438230 (+) 2055 WP_243989452.1 type IV pilus secretin PilQ Machinery gene
  MT343_RS01895 aroK 438234..438749 (+) 516 WP_145470740.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 365 a.a.        Molecular weight: 39953.96 Da        Isoelectric Point: 4.2157

>NTDB_id=673844 MT343_RS01870 WP_366516530.1 433285..434382(+) (pilM) [Thalassolituus sp. C2-1]
MQVREFDGDFVVLANLFNKKNKAILGVDISSTSVKVLELSRNNDRYQVEAYAAEPLPANAVVEQSISNDEAVGEAIRRAL
SRSRASAKRAAIAVAGSAVITKTVQMNGKLKDDEMDFQIRAEADQYIPYPLEEVALDWEIQGPSDSGNDMVDVLLAACRS
ETVERRKDAVEYANLEAAVVDVEAFCTERAFSLLAGQLDGDEIETVAILDIGATMTTLSVLHEGKSIYTREQLFGGKQLS
EDIMRRYGLSEEEATRAKLEGGLPDDYESEVLEPFRRAVVQQVSRSLQFFYSSSQFNDVDYIILAGGTSSIPYLSDQVQD
AMGIATVIANPFVNMTLSQKVNANLLNGDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1098 bp        

>NTDB_id=673844 MT343_RS01870 WP_366516530.1 433285..434382(+) (pilM) [Thalassolituus sp. C2-1]
GTGCAAGTTCGCGAATTTGATGGGGATTTTGTCGTGCTGGCTAACTTGTTTAATAAGAAGAACAAGGCAATTCTCGGGGT
GGATATCAGTTCCACCTCGGTAAAAGTCCTTGAGTTGAGTCGCAATAACGATCGCTATCAGGTTGAAGCTTATGCCGCTG
AACCCCTGCCTGCCAACGCTGTGGTTGAACAAAGCATCAGCAATGACGAAGCGGTTGGCGAAGCAATACGTCGTGCTTTG
AGTCGTTCCCGTGCCAGTGCCAAGCGTGCAGCTATTGCTGTTGCCGGCTCTGCGGTAATCACCAAAACAGTGCAGATGAA
CGGCAAGCTGAAAGATGATGAAATGGACTTCCAGATCCGCGCTGAAGCGGATCAGTATATTCCCTATCCTCTGGAAGAAG
TTGCGCTGGATTGGGAAATCCAGGGCCCGTCCGACTCTGGCAATGACATGGTAGATGTTCTGCTGGCGGCCTGCCGTTCT
GAAACCGTTGAGCGCCGTAAAGACGCGGTTGAATACGCTAACCTCGAAGCTGCGGTTGTGGATGTTGAAGCTTTCTGTAC
CGAACGCGCCTTCTCTTTATTGGCAGGTCAGCTGGATGGTGACGAGATTGAAACCGTCGCTATTCTGGATATCGGTGCGA
CTATGACCACGCTCAGCGTGTTGCATGAAGGCAAATCCATTTATACCCGTGAACAGTTATTCGGTGGTAAGCAGCTGAGT
GAAGATATCATGCGCCGCTATGGTCTCAGTGAAGAAGAAGCGACACGGGCTAAGCTGGAAGGTGGTTTACCGGATGACTA
CGAAAGTGAGGTTCTGGAGCCTTTCCGCCGTGCGGTAGTTCAGCAGGTCAGCCGTTCGCTGCAGTTTTTTTACTCGTCCA
GCCAGTTTAATGATGTTGATTACATCATTCTTGCCGGTGGTACTTCTTCTATCCCCTATCTGTCGGACCAGGTTCAGGAC
GCTATGGGTATCGCAACTGTGATTGCCAATCCCTTTGTGAATATGACGCTGTCGCAGAAGGTTAACGCGAATCTGCTGAA
TGGCGACGCGCCTTCGTTGATGATTGCGTGCGGGCTGGCGATGAGGAGCTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(26-364)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.857

95.89

0.526
  comM Acinetobacter nosocomialis M2

54.857

95.89

0.526
  comM Acinetobacter baylyi ADP1

50.708

96.712

0.49
  pilM Legionella pneumophila strain ERS1305867

43.785

96.986

0.425