Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MUG10_RS15690 Genome accession   NZ_CP094827
Coordinates   3534175..3535911 (+) Length   578 a.a.
NCBI ID   WP_252162276.1    Uniprot ID   A0A9Q9MQQ4
Organism   Xanthomonas prunicola strain Arg-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3529175..3540911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUG10_RS15670 (MUG10_15675) pilA 3529858..3530283 (+) 426 WP_252162273.1 pilin Machinery gene
  MUG10_RS15675 (MUG10_15680) - 3530418..3532004 (+) 1587 WP_252162274.1 phosphoethanolamine transferase -
  MUG10_RS15680 (MUG10_15685) - 3532015..3532644 (+) 630 WP_252165100.1 hypothetical protein -
  MUG10_RS15685 (MUG10_15690) - 3532614..3534140 (+) 1527 WP_313905434.1 hypothetical protein -
  MUG10_RS15690 (MUG10_15695) pilB 3534175..3535911 (+) 1737 WP_252162276.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MUG10_RS15695 (MUG10_15700) pilR 3536583..3538037 (-) 1455 WP_286365574.1 sigma-54 dependent transcriptional regulator Regulator
  MUG10_RS15700 (MUG10_15705) - 3538308..3539920 (-) 1613 Protein_3057 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62785.89 Da        Isoelectric Point: 5.4491

>NTDB_id=673507 MUG10_RS15690 WP_252162276.1 3534175..3535911(+) (pilB) [Xanthomonas prunicola strain Arg-1]
MNSVATTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKKLVSASQLAAANALEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQANNASFGSSMG
DDDDEMGELDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=673507 MUG10_RS15690 WP_252162276.1 3534175..3535911(+) (pilB) [Xanthomonas prunicola strain Arg-1]
ATGAATTCAGTAGCTACCACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGGACGGCAATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAAGCTGG
TGTCGGCGTCGCAGCTGGCGGCGGCCAATGCCTTGGAATTCGGCATGCCGCTGATGGATGTGTCTGTATTCGACGCTAGC
CAGAACGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACGAACCTGGTGGTCGAGGCTA
TCCTGGTGGACGAGGACCAGATTCGTCGCACCTTGGAGCAGTGGCAGGCCAATAACGCTTCGTTTGGCTCGTCAATGGGG
GACGACGACGACGAAATGGGGGAGCTGGACGTCTCCACCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAAGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGTGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGATTACCGGGTGCGCTTGCGGATCGATGGCCTGCTGAAGAATGTGGCCAAGGCGCCG
GTCAAGCTGAGCCAGCGCATCGCCGCCCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTTCGCGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCAGCCAAGCTGGGCATCGAGAAGCTGGGTTACGAAGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAGACGGTGTCGCT
GTATACGGCCTTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GCGTCAATCAGGTCCAGCAGAACAACAAGCGCGGCATGACGTTTGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACTGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCGACGCTGCACACCAACGATGCACCGCAGACCATCGCGCGCTTGATGAACATGGGCATTGCGCCGTACAACATCA
CTTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGTCGGTTGTGCAACAACTGCAAGCGTAAGTCGACCTTGCCC
GAGCATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAAATCGCTGCCGGTATCGAACTGTACGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAAAGTATCGGCATCCGTGATCTGCGCCAGTCGGCCTTGATG
AAGGCCGCGCATGGGGTGACCAGCCTGGCGGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.13

99.481

0.548
  pilB Legionella pneumophila strain ERS1305867

52.548

98.443

0.517
  pilB Vibrio cholerae strain A1552

49.306

99.654

0.491
  pilF Neisseria gonorrhoeae MS11

48.415

98.27

0.476
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

97.578

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.734

91.003

0.362