Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MUA35_RS07725 Genome accession   NZ_CP094770
Coordinates   1584815..1585789 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain IVB6201     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1579815..1590789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA35_RS07685 (MUA35_07660) gcvT 1580316..1581407 (-) 1092 WP_262538681.1 glycine cleavage system aminomethyltransferase GcvT -
  MUA35_RS07690 (MUA35_07665) - 1581566..1582090 (-) 525 WP_001015120.1 shikimate kinase -
  MUA35_RS07695 (MUA35_07670) - 1582080..1582226 (-) 147 WP_001789879.1 hypothetical protein -
  MUA35_RS07700 (MUA35_07675) comGF 1582323..1582820 (-) 498 WP_262607516.1 competence type IV pilus minor pilin ComGF Machinery gene
  MUA35_RS07705 (MUA35_07680) comGE 1582738..1583037 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  MUA35_RS07710 (MUA35_07685) comGD 1583024..1583470 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  MUA35_RS07715 (MUA35_07690) comGC 1583448..1583759 (-) 312 WP_262538680.1 competence type IV pilus major pilin ComGC Machinery gene
  MUA35_RS07720 (MUA35_07695) comGB 1583773..1584843 (-) 1071 WP_262538679.1 competence type IV pilus assembly protein ComGB Machinery gene
  MUA35_RS07725 (MUA35_07700) comGA 1584815..1585789 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  MUA35_RS07730 (MUA35_07705) - 1585841..1586464 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MUA35_RS07735 (MUA35_07710) - 1586461..1586790 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MUA35_RS07740 (MUA35_07715) - 1586790..1587776 (-) 987 WP_000161315.1 ROK family glucokinase -
  MUA35_RS07745 (MUA35_07720) - 1587773..1587976 (-) 204 WP_000087561.1 YqgQ family protein -
  MUA35_RS07750 (MUA35_07725) - 1587957..1589420 (-) 1464 WP_250743365.1 rhomboid family intramembrane serine protease -
  MUA35_RS07755 (MUA35_07730) - 1589432..1589971 (-) 540 WP_000164547.1 5-formyltetrahydrofolate cyclo-ligase -
  MUA35_RS07760 (MUA35_07735) rpmG 1590180..1590329 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=672213 MUA35_RS07725 WP_000697220.1 1584815..1585789(-) (comGA) [Staphylococcus aureus strain IVB6201]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=672213 MUA35_RS07725 WP_000697220.1 1584815..1585789(-) (comGA) [Staphylococcus aureus strain IVB6201]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTACCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1