Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   MRB47_RS18855 Genome accession   NZ_CP094346
Coordinates   4044046..4044579 (-) Length   177 a.a.
NCBI ID   WP_411275717.1    Uniprot ID   -
Organism   Diaphorobacter sp. LI3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4039046..4049579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRB47_RS18840 (MRB47_18840) - 4039054..4040073 (-) 1020 WP_252100635.1 IS30 family transposase -
  MRB47_RS18845 (MRB47_18845) - 4040147..4043170 (-) 3024 WP_252101465.1 fused MFS/spermidine synthase -
  MRB47_RS18850 (MRB47_18850) - 4043228..4043992 (-) 765 WP_252101466.1 hypothetical protein -
  MRB47_RS18855 (MRB47_18855) pilE 4044046..4044579 (-) 534 WP_411275717.1 pilin Machinery gene
  MRB47_RS18865 (MRB47_18865) - 4044923..4045558 (-) 636 WP_252101467.1 FHA domain-containing protein -
  MRB47_RS18870 (MRB47_18870) - 4045663..4046478 (-) 816 WP_252101468.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  MRB47_RS18875 (MRB47_18875) - 4046591..4047553 (-) 963 WP_011804582.1 IS110 family transposase -
  MRB47_RS18880 (MRB47_18880) - 4047944..4048627 (-) 684 WP_252101469.1 TerC family protein -

Sequence

Protein


Download         Length: 177 a.a.        Molecular weight: 18132.84 Da        Isoelectric Point: 9.4832

>NTDB_id=669157 MRB47_RS18855 WP_411275717.1 4044046..4044579(-) (pilE) [Diaphorobacter sp. LI3]
MKRTLQQGFTLIELMIVVAIIGILAAIALPAYQDYTVRTRVSEGLSLAESAKSMLASDATSVNDLANVVTTWNAQAGNTG
ANSKFVEKVQIGGTGEITVTYRAAAVGVAAAKNTVNINPFVRDTAGAPQQLKAALTAGVSGALDWACASATQVAATKAGM
KATAGTLEAKYAPAICR

Nucleotide


Download         Length: 534 bp        

>NTDB_id=669157 MRB47_RS18855 WP_411275717.1 4044046..4044579(-) (pilE) [Diaphorobacter sp. LI3]
ATGAAGCGTACCCTGCAACAAGGTTTCACCCTGATCGAATTGATGATCGTTGTGGCGATCATTGGTATCTTGGCTGCCAT
TGCGCTGCCTGCTTACCAGGACTACACAGTCCGCACCCGAGTTTCTGAAGGCTTGAGCCTTGCCGAAAGTGCAAAAAGCA
TGCTGGCCAGCGATGCTACGTCTGTCAATGATCTGGCGAACGTGGTGACCACTTGGAATGCACAAGCAGGCAATACTGGT
GCAAATTCTAAGTTTGTTGAAAAAGTGCAAATCGGTGGTACGGGTGAAATCACGGTGACCTACCGTGCTGCCGCAGTCGG
TGTGGCTGCCGCAAAAAATACCGTGAATATTAACCCGTTTGTGCGCGATACTGCTGGTGCGCCACAACAGCTGAAGGCCG
CATTGACGGCAGGGGTGAGCGGAGCGCTCGACTGGGCTTGCGCTTCGGCGACCCAAGTTGCTGCCACGAAAGCAGGCATG
AAGGCAACAGCGGGTACACTGGAAGCGAAGTACGCTCCTGCTATTTGCCGATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)

Pilin (bacterial filament) (Pilin)

(37-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.714

100

0.48
  pilA Ralstonia pseudosolanacearum GMI1000

41.398

100

0.435
  pilA2 Legionella pneumophila strain ERS1305867

43.023

97.175

0.418
  pilA2 Legionella pneumophila str. Paris

43.023

97.175

0.418
  pilE Neisseria gonorrhoeae strain FA1090

36.813

100

0.379

Multiple sequence alignment