Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MN200_RS08660 Genome accession   NZ_CP093968
Coordinates   1769792..1770985 (-) Length   397 a.a.
NCBI ID   WP_242759180.1    Uniprot ID   -
Organism   Acinetobacter sp. LUNF3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1764792..1775985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MN200_RS08645 (MN200_08645) - 1766832..1767860 (+) 1029 WP_004979806.1 AraC family transcriptional regulator -
  MN200_RS08650 (MN200_08650) gcvH 1767932..1768306 (+) 375 WP_176537700.1 glycine cleavage system protein GcvH -
  MN200_RS08655 (MN200_08655) - 1768567..1769745 (+) 1179 WP_242759178.1 MFS transporter -
  MN200_RS08660 (MN200_08660) pilU 1769792..1770985 (-) 1194 WP_242759180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MN200_RS08665 (MN200_08665) - 1771224..1772285 (-) 1062 WP_010326643.1 3-deoxy-7-phosphoheptulonate synthase -
  MN200_RS08670 (MN200_08670) - 1772764..1774863 (-) 2100 WP_242759182.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 44720.09 Da        Isoelectric Point: 6.3921

>NTDB_id=666090 MN200_RS08660 WP_242759180.1 1769792..1770985(-) (pilU) [Acinetobacter sp. LUNF3]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRINVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLNSSSALSFDQAVAEKAKEAEEEKDDA

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=666090 MN200_RS08660 WP_242759180.1 1769792..1770985(-) (pilU) [Acinetobacter sp. LUNF3]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGCTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTTATGAAGCCATTGGGTCAACAAAATTTGC
CATCTGATCAGACTAAGCTCTTTGCTTATAGCTTAATGAACGAAAAGCAACGATTGGAGTTCGAAACTGAGCTTGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTTTCGCGTTTTCGTATCAATGTTTTTCAGCAGCAACTGCATGTGGGGATGGTGAT
TCGTACGATTACCGCGGAAATTCCTAACTTCACCAAGTTACAACTTCCAACGTCTTTAAAAGACGTGATTATGGAAAAAC
GTGGCTTAGTATTGGTAGTTGGGGGTACAGGGTCGGGTAAATCTACGTCTTTGGCGGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACGGTTGAAGACCCAGTGGAATATGTTCACAAGCATAAGAAATCGATGATTACTCATCG
GGAAGTGGGTGTAGACTGTCATTCTTGGCACAATGCTTTGAAAAATACTTTGCGCCAAGCGCCCGATGTCATTTTAATTG
GTGAAATTCGTGACACGGAGACTATGGAGCATGCCATAGCTTTTGCAGAAACAGGCCACTTATGCCTAGGCACATTACAT
GCTAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGTCTGGTGCGAACTGAAGATGGTCGTGGTCGCCGAGCAGCAGTGGAAA
TCATGCTCAATACGCCGTTGATGTCAGACTTGATACTTAAAGGGAATTTCCATGAACTCAAAGAAGTGATGTCGAAGTCA
CGAGAGCTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATTGCTTACGAAGAAGCGCT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAATTAAAGAGTAGTCGTGCTAACCCACAGTTAAACTCAA
GTAGTGCTTTGTCTTTTGATCAAGCGGTTGCCGAAAAGGCCAAAGAAGCTGAAGAAGAGAAAGACGACGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.053

90.428

0.534
  pilU Acinetobacter baylyi ADP1

54.545

91.436

0.499
  pilU Vibrio cholerae strain A1552

50.289

87.154

0.438
  pilT Legionella pneumophila strain Lp02

42.183

85.39

0.36
  pilT Legionella pneumophila strain ERS1305867

42.183

85.39

0.36