NTDB

Overview

Name	comM	Type	Machinery gene
Locus tag	MO982_RS27530	Genome accession	NZ_CP093966
Coordinates	5948018..5949511 (+)	Length	497 a.a.
NCBI ID	WP_003098248.1	Uniprot ID	Q9HTR4
Organism	Pseudomonas aeruginosa strain ATCC BAA-2114
Function	require for natural transformation (predicted from homology) Unclear

Genomic Context

Location: 5943018..5954511

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MO982_RS27500 (MO982_27495)	-	5943161..5944075 (-)	915	WP_003118059.1	fimbrial protein	-
MO982_RS27505 (MO982_27500)	sutA	5944490..5944807 (-)	318	WP_003098240.1	transcriptional regulator SutA	-
MO982_RS27510 (MO982_27505)	-	5944885..5945310 (-)	426	WP_003096441.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
MO982_RS27515 (MO982_27510)	-	5945571..5946899 (-)	1329	WP_016562483.1	ammonium transporter	-
MO982_RS27520 (MO982_27515)	glnK	5946939..5947277 (-)	339	WP_003096476.1	P-II family nitrogen regulator	-
MO982_RS27525 (MO982_27520)	-	5947717..5947977 (+)	261	WP_093976207.1	accessory factor UbiK family protein	-
MO982_RS27530 (MO982_27525)	comM	5948018..5949511 (+)	1494	WP_003098248.1	YifB family Mg chelatase-like AAA ATPase	Machinery gene
MO982_RS27535 (MO982_27530)	betT	5949636..5951621 (+)	1986	WP_003096496.1	choline BCCT transporter BetT	-
MO982_RS27540 (MO982_27535)	pchP	5951664..5952713 (-)	1050	WP_093976208.1	phosphorylcholine phosphatase	-
MO982_RS27545 (MO982_27540)	-	5952864..5953781 (-)	918	WP_003098255.1	LysR substrate-binding domain-containing protein	-

Sequence

Protein

Download Length: 497 a.a. Molecular weight: 53083.04 Da Isoelectric Point: 7.9587

                                >NTDB_id=666030 MO982_RS27530 WP_003098248.1 5948018..5949511(+) (comM) [Pseudomonas aeruginosa strain ATCC BAA-2114]

MSLAIVHSRAQVGVEAPCVSVEAHLANGLPSLTLVGLPETAVRESKDRVRSALLNAGFDFPARRITLNLAPADLPKDGGR

FDLAIALGILAASGQLPGTALDGLECLGELALSGAIRPVRGVLPAALAARDARRVLVVPKENAEEASLASGLTVFAVDHL

LEIAGHLSGQAPLPPYQARGLLRAPFPYPDLAEVQGQAAAKRALLVAAAGAHNLLLSGPPGTGKTLLASRLPGLLPALDE

DEALEVAAIHSVASHVPLRHWPQRPFRQPHHSASAPALVGGGSRPQPGEITLAHQGVLFLDELPEFERKVLEVLREPLES

GEIVIARANGRVRFPARFQLVAAMNPCPCGYLGDPSGRCRCTPEQVQRYRGKLSGPLLDRIDLHVSVLRESTSLQPGHGE

TATAEVSERVGAARQRQLARQGCANAHLDLQAMHRNCALAEADRRWLEAAGERLELSLRALHRILKVARTLADLERIDAI

ERRHLAEALQYRAMTST

Nucleotide

Download Length: 1494 bp

                                >NTDB_id=666030 MO982_RS27530 WP_003098248.1 5948018..5949511(+) (comM) [Pseudomonas aeruginosa strain ATCC BAA-2114]

ATGTCCCTGGCGATTGTCCACAGCCGAGCCCAGGTCGGCGTCGAAGCCCCCTGCGTCAGCGTCGAGGCGCACCTGGCCAA

CGGCCTGCCTTCGCTGACCCTGGTCGGCCTGCCGGAAACCGCGGTGCGCGAGAGCAAGGACCGCGTGCGCAGCGCCCTGC

TCAATGCCGGTTTCGACTTCCCCGCGCGGCGCATCACCCTCAACCTCGCCCCCGCCGACCTACCCAAGGACGGCGGTCGC

TTCGACCTGGCCATCGCACTCGGCATCCTCGCCGCCAGCGGCCAGTTGCCCGGCACCGCCCTCGACGGCCTGGAGTGCCT

CGGCGAACTGGCCCTGTCCGGGGCGATCCGGCCAGTGCGAGGCGTATTGCCGGCCGCGCTGGCGGCGCGCGACGCAAGGC

GCGTTCTGGTGGTACCGAAGGAAAATGCCGAAGAGGCCAGCCTGGCCAGCGGGCTGACGGTGTTCGCCGTGGACCACCTG

CTGGAGATCGCCGGACACCTCTCCGGCCAGGCCCCGCTGCCGCCCTACCAGGCCCGCGGCCTGCTCCGCGCGCCCTTCCC

TTATCCAGACCTGGCCGAGGTCCAGGGCCAGGCCGCCGCCAAGCGCGCCCTGCTGGTAGCCGCCGCCGGCGCGCACAACC

TGTTGCTCAGCGGCCCGCCGGGCACCGGCAAGACCCTCCTGGCCAGCCGCCTGCCCGGCCTGCTGCCGGCGCTCGACGAG

GACGAAGCCCTGGAGGTCGCGGCGATCCATTCGGTGGCCAGCCACGTCCCCCTCAGGCACTGGCCGCAGCGACCGTTCCG

CCAGCCGCACCACTCCGCCTCCGCGCCGGCCCTGGTCGGCGGCGGCAGCCGCCCGCAGCCGGGCGAGATCACCCTGGCGC

ACCAGGGCGTGCTGTTCCTCGACGAACTGCCGGAGTTCGAGCGCAAGGTCCTGGAGGTCCTGCGCGAGCCGCTGGAAAGC

GGCGAGATCGTCATTGCCCGGGCCAACGGCCGGGTACGTTTCCCGGCGCGCTTCCAACTGGTGGCGGCGATGAATCCCTG

TCCCTGTGGCTACCTCGGCGATCCCAGTGGCCGCTGCCGCTGCACCCCGGAACAGGTCCAGCGCTACCGGGGCAAGCTGT

CCGGACCGCTGCTCGATCGCATCGACCTGCACGTCAGCGTGCTCCGCGAAAGCACCAGCCTGCAGCCAGGACACGGCGAA

ACCGCTACCGCCGAGGTCAGCGAACGGGTGGGCGCCGCACGGCAACGGCAACTGGCCCGCCAGGGCTGCGCCAATGCCCA

TCTCGATCTCCAGGCGATGCACCGCAATTGTGCACTCGCCGAAGCGGACCGCCGCTGGCTGGAGGCTGCCGGAGAGCGCC

TGGAACTTTCCTTGCGCGCCTTGCATCGCATACTCAAGGTGGCCCGGACGCTGGCCGACCTGGAGCGCATCGATGCCATC

GAACGCCGGCACCTGGCGGAAGCCCTGCAGTATCGGGCAATGACCTCCACGTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(190-390)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(402-492)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q9HTR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comM	Haemophilus influenzae Rd KW20	55.186	100	0.567
comM	Vibrio campbellii strain DS40M4	56.74	100	0.567
comM	Vibrio cholerae strain A1552	56.338	100	0.563
comM	Glaesserella parasuis strain SC1401	55.179	100	0.557
comM	Legionella pneumophila str. Paris	50.1	100	0.503
comM	Legionella pneumophila strain ERS1305867	50.1	100	0.503
RA0C_RS07335	Riemerella anatipestifer ATCC 11845 = DSM 15868	45.866	100	0.469

Detailed information NTG0666030