Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MO327_RS05215 Genome accession   NZ_CP093449
Coordinates   1208126..1209862 (+) Length   578 a.a.
NCBI ID   WP_058195835.1    Uniprot ID   -
Organism   Xanthomonas translucens strain CIX162     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1210486..1220463 1208126..1209862 flank 624


Gene organization within MGE regions


Location: 1208126..1220463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MO327_RS05215 (MO327_05200) pilB 1208126..1209862 (+) 1737 WP_058195835.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MO327_RS05220 (MO327_05205) - 1210037..1210489 (-) 453 WP_304571469.1 Fic family protein -
  MO327_RS05225 (MO327_05210) - 1210486..1210668 (-) 183 WP_003466380.1 YhfG family protein -
  MO327_RS05230 (MO327_05215) pilR 1210857..1212245 (-) 1389 WP_003466378.1 sigma-54 dependent transcriptional regulator Regulator
  MO327_RS05235 (MO327_05220) - 1212320..1213933 (-) 1614 WP_058361135.1 ATP-binding protein -
  MO327_RS05240 (MO327_05225) sucC 1214174..1215337 (+) 1164 WP_003466374.1 ADP-forming succinate--CoA ligase subunit beta -
  MO327_RS05245 (MO327_05230) sucD 1215361..1216233 (+) 873 WP_003466372.1 succinate--CoA ligase subunit alpha -
  MO327_RS05250 (MO327_05235) - 1216519..1217817 (+) 1299 WP_304571470.1 hypothetical protein -
  MO327_RS05255 (MO327_05240) - 1217766..1218071 (+) 306 WP_003466368.1 PQQ-binding-like beta-propeller repeat protein -
  MO327_RS05260 (MO327_05245) - 1218184..1220463 (-) 2280 WP_304571471.1 hypothetical protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62906.19 Da        Isoelectric Point: 6.2661

>NTDB_id=664760 MO327_RS05215 WP_058195835.1 1208126..1209862(+) (pilB) [Xanthomonas translucens strain CIX162]
MNAVTSANLVGITGIARRLVQDGALEETAARTAMAHAAEAKIPLPQWFSDKKLVTAAQLAAANALEFGMPLLDVSVFDAS
QSAMKLVSEELVHKHQVLPLFKRGNRLFVGMSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGDALG
DDDEAMGRLEVGAGDEDMGSGGETGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVARAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
HNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIAQAAQKIGVKDLRQSALL
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=664760 MO327_RS05215 WP_058195835.1 1208126..1209862(+) (pilB) [Xanthomonas translucens strain CIX162]
ATGAACGCTGTGACATCCGCCAATCTCGTCGGCATCACCGGCATCGCCCGCCGCCTGGTTCAGGATGGCGCGCTCGAGGA
AACGGCTGCGCGGACCGCGATGGCGCATGCCGCCGAGGCGAAGATCCCGCTGCCGCAGTGGTTCTCGGACAAGAAGCTGG
TCACCGCGGCGCAGCTGGCCGCCGCCAATGCGCTGGAGTTCGGGATGCCGTTGCTGGACGTGTCGGTGTTCGATGCCAGC
CAGAGCGCGATGAAGCTGGTCAGCGAGGAGCTGGTGCACAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGTCT
GTTCGTCGGGATGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACGGTGGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCGAGCAACGATGCGATCGGCGACGCGCTGGGC
GACGACGACGAGGCCATGGGCAGGCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCAGCGGCGGCGAGACCGGCGTCGA
TGCCAAGGGCGACGATACGCCCGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCCTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAGGGCGCCG
GTCAAGCTCAACCAGCGCATTGCGGCGCGGCTCAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
CGGACGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACGCTGCCGACCTTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAGAAA
CTGTTCCTGGACGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACTGCGCTGGGCATCCTCAACGACGAGACCCGCAACATCTCCACGGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGGCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACGCTGGTGATCGCGCAGCGGCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCCCGACCCAGCTGCCG
CACAATGCGCTGCTGGCCGAGGGCTTCAGCGAGGCGGAAGTGGCCGCCGGCATCCAGCTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCGATCGTGC
TGCAGGGCGGCAACGCGATGGACATCGCCCAGGCCGCGCAGAAGATCGGGGTCAAGGACCTGCGCCAGTCGGCGCTGCTC
AAGGCGCGCGCCGGCATCACCAGCCTGGCGGAGATCAATCGCGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.754

98.27

0.538
  pilB Acinetobacter baumannii D1279779

54.276

99.135

0.538
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

48.958

99.654

0.488
  pilF Neisseria gonorrhoeae MS11

48.768

98.27

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.643

97.924

0.457
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilF Thermus thermophilus HB27

40.385

89.965

0.363