Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OG510_RS35100 Genome accession   NZ_CP108660
Coordinates   7794399..7795511 (-) Length   370 a.a.
NCBI ID   WP_406142057.1    Uniprot ID   -
Organism   Streptomyces sp. NBC_01089     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7789399..7800511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OG510_RS35075 (OG510_35015) - 7789745..7789960 (-) 216 WP_406142052.1 hypothetical protein -
  OG510_RS35080 (OG510_35020) - 7790003..7790689 (-) 687 WP_406142053.1 TetR/AcrR family transcriptional regulator -
  OG510_RS35085 (OG510_35025) - 7790794..7791855 (+) 1062 WP_406142054.1 medium chain dehydrogenase/reductase family protein -
  OG510_RS35090 (OG510_35030) - 7792121..7792960 (-) 840 WP_406142055.1 aldo/keto reductase -
  OG510_RS35095 (OG510_35035) - 7793196..7794386 (+) 1191 WP_406142056.1 acyl-CoA dehydrogenase family protein -
  OG510_RS35100 (OG510_35040) pilT 7794399..7795511 (-) 1113 WP_406142057.1 type IV pilus twitching motility protein PilT Machinery gene
  OG510_RS35105 (OG510_35045) - 7795508..7799332 (-) 3825 WP_406142058.1 hypothetical protein -
  OG510_RS35110 (OG510_35050) - 7799329..7799853 (-) 525 WP_406142059.1 type II secretion system protein -
  OG510_RS35115 (OG510_35055) - 7799850..7800284 (-) 435 WP_406142060.1 type II secretion system protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 39985.57 Da        Isoelectric Point: 6.4510

>NTDB_id=664708 OG510_RS35100 WP_406142057.1 7794399..7795511(-) (pilT) [Streptomyces sp. NBC_01089]
MSTHATLETTMPGPSSAIIDHLLGELWERGGSDLLLTAGSAPFLRVDGALRPVEGAALTEPEVDRLVTGVLGGALTERFR
REKQVDFAFSWGEKARLRGNAFVQRGVSALALRIIPFEVPSSEQLGLPPVVIGWAGMPRGFVLVTGPTGSGKSTSLAALL
DYVNTHRPVHILTIEDPIEYLHRHKHSAVNQREVGTDTDSFPAALRSALREDPDVLLLGEMRDPESISAALTIAETGHLV
FATLHTNDTSQTLDRIVDVFPAEQQPQIRLQLAHTLVGILNQTLIPRIGGGRVAAFEVLVGTSAIRNLIREGKTRQIRNM
VSTSHRDGMQTLETSINALVAAGTIAHEEAVLHTAHPEDIQRPHVPVGRA

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=664708 OG510_RS35100 WP_406142057.1 7794399..7795511(-) (pilT) [Streptomyces sp. NBC_01089]
ATGAGCACACACGCCACCTTGGAGACCACCATGCCCGGGCCGAGTTCCGCGATCATCGACCACCTGCTCGGTGAGCTGTG
GGAGCGGGGCGGCAGCGACCTGCTGCTCACCGCCGGATCGGCGCCGTTCCTCCGCGTCGACGGCGCCCTGCGACCGGTCG
AGGGGGCCGCGCTGACCGAGCCTGAGGTCGACCGCCTGGTGACCGGGGTGCTGGGCGGGGCACTCACGGAACGGTTCCGC
CGGGAGAAGCAGGTCGATTTCGCGTTCAGCTGGGGGGAGAAGGCCAGGTTGCGCGGGAACGCTTTCGTGCAGCGCGGCGT
GTCGGCGCTGGCGCTGCGGATCATCCCGTTCGAGGTGCCGTCGTCCGAGCAGCTCGGGCTCCCCCCGGTCGTCATCGGGT
GGGCGGGCATGCCGCGCGGGTTCGTGCTGGTCACCGGGCCGACCGGGTCGGGCAAGTCGACGAGCCTGGCGGCGCTCCTC
GACTACGTCAACACGCACAGGCCGGTGCACATCCTCACCATCGAGGACCCGATCGAGTACCTGCACCGGCACAAGCACTC
GGCGGTCAACCAGCGGGAGGTCGGCACCGACACCGACTCGTTCCCCGCCGCGCTGCGCTCGGCGCTGCGCGAGGATCCCG
ACGTCCTGCTCCTCGGCGAGATGCGCGACCCGGAGAGCATCTCCGCGGCCCTGACCATCGCCGAGACCGGGCACCTGGTG
TTCGCCACCCTGCACACCAACGACACGTCGCAGACGCTGGACCGCATCGTCGATGTGTTCCCGGCCGAGCAACAACCGCA
GATCCGATTGCAGTTGGCGCACACCCTGGTCGGCATTCTCAACCAGACACTCATCCCCCGGATCGGCGGGGGCCGGGTGG
CGGCCTTCGAGGTACTGGTGGGCACGTCGGCGATCCGCAACCTGATCCGCGAGGGCAAGACCCGCCAGATCCGCAACATG
GTCTCGACCAGCCACCGTGACGGCATGCAGACCCTGGAGACCAGCATCAACGCTCTGGTCGCGGCAGGCACCATCGCCCA
CGAGGAGGCCGTGCTGCACACGGCCCACCCGGAGGACATCCAGCGGCCCCACGTCCCGGTCGGCCGCGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(138-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

49.415

92.432

0.457
  pilT Acinetobacter baumannii strain A118

48.529

91.892

0.446
  pilT Acinetobacter baumannii D1279779

48.529

91.892

0.446
  pilT Pseudomonas stutzeri DSM 10701

47.953

92.432

0.443
  pilT Acinetobacter nosocomialis M2

48.235

91.892

0.443
  pilT Neisseria meningitidis 8013

48.802

90.27

0.441
  pilT Legionella pneumophila strain ERS1305867

47.929

91.351

0.438
  pilT Legionella pneumophila strain Lp02

47.929

91.351

0.438
  pilT Neisseria gonorrhoeae MS11

48.503

90.27

0.438
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.917

90.811

0.435
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.494

89.73

0.435
  pilT Vibrio cholerae strain A1552

48.494

89.73

0.435
  pilT Acinetobacter baylyi ADP1

48.93

88.378

0.432
  pilU Pseudomonas stutzeri DSM 10701

42.029

93.243

0.392
  pilU Vibrio cholerae strain A1552

40.828

91.351

0.373
  pilU Acinetobacter baylyi ADP1

38.841

93.243

0.362