Detailed information NTG0066443

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: PSCI_RS11265
• Genome accession: NZ_AP014637
• Coordinates: 2458015..2459079 (+)
• Length: 354 a.a.
• NCBI ID: WP_045486514.1
• Uniprot ID: A0A077LFT9
• Organism: Pseudomonas sp. StFLB209
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2453015..2464079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSCI_RS11250 (PSCI_2227)	rpmE	2453354..2453575 (+)	222	WP_045486504.1	50S ribosomal protein L31	-
PSCI_RS11255 (PSCI_2228)	-	2453993..2455261 (+)	1269	WP_045486508.1	malic enzyme-like NAD(P)-binding protein	-
PSCI_RS11260 (PSCI_2229)	-	2455367..2457808 (-)	2442	WP_045486511.1	penicillin-binding protein 1A	-
PSCI_RS11265 (PSCI_2230)	comM	2458015..2459079 (+)	1065	WP_045486514.1	pilus assembly protein PilM	Machinery gene
PSCI_RS11270 (PSCI_2231)	-	2459079..2459639 (+)	561	WP_045486517.1	PilN domain-containing protein	-
PSCI_RS11275 (PSCI_2232)	pilO	2459636..2460259 (+)	624	WP_045486520.1	type 4a pilus biogenesis protein PilO	-
PSCI_RS11280 (PSCI_2233)	-	2460256..2460783 (+)	528	WP_045486523.1	pilus assembly protein PilP	-
PSCI_RS11285 (PSCI_2234)	pilQ	2460831..2462996 (+)	2166	WP_045486527.1	type IV pilus secretin PilQ	Machinery gene
PSCI_RS11290 (PSCI_2235)	aroK	2463001..2463519 (+)	519	WP_045494141.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37955.60 Da
• Isoelectric Point: 4.9190

>NTDB_id=66443 PSCI_RS11265 WP_045486514.1 2458015..2459079(+) (comM) [Pseudomonas sp. StFLB209]
MFELFSKKAHTLLGIDISSTAVKLLELSRSGSRYKVESYAVEPLPANAVVEKNIAELEGVGSALGRVVVKARTNVKNAAV
AVAGSAVITKVVEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFSLLSAQLGKGHEMLTVAVVDIGATMTTLSVLHQGRIIYTREQLFGGKQLTEEIQRRYGLSM
GEAGLAKKQGGLPDDYGSEVLEPFKDAMVQQVSRSLQFFFAAGQHNSVDYIMLAGGSASIAGLEHLIQKRLGVPTMVANP
FADMALSSKVNAGALASDAPALMLACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=66443 PSCI_RS11265 WP_045486514.1 2458015..2459079(+) (comM) [Pseudomonas sp. StFLB209]
GTGTTCGAACTCTTCAGCAAGAAGGCCCACACCCTTCTAGGGATCGATATTAGCTCCACTGCGGTAAAACTCCTCGAACT
GAGTCGTTCCGGCAGCCGTTACAAAGTCGAGTCCTATGCGGTCGAACCATTGCCGGCCAATGCTGTGGTTGAAAAGAACA
TTGCCGAGCTCGAAGGCGTCGGAAGCGCGTTGGGGCGCGTCGTGGTCAAGGCCCGTACCAACGTCAAGAATGCGGCGGTG
GCGGTGGCGGGGTCTGCCGTGATCACCAAGGTGGTCGAGATGGATGCCGGGTTGTCGGATGATGACATGGAGAACCAGCT
CAAGCTCGAAGCCGACCAGTACATCCCCTACCCGCTGGAAGAGGTCGCCATCGATTTCGAGGTGCAGGGGTATTCGGTGC
GCAACCCCGAGCGCGTCGAGGTCCTGCTGGCCGCGTGTCGCAAGGAGAACGTCGAGGTCCGCGAAGCCGCCCTGGCGCTG
GCCGGCCTGACCGCCCGGGTCGTCGACGTCGAGGCTTATGCGCTGGAGCGCTCGTTCAGCCTGTTGTCGGCCCAACTGGG
CAAAGGCCATGAAATGCTCACCGTTGCGGTCGTCGATATCGGCGCGACCATGACCACGCTGAGTGTGCTCCACCAGGGAC
GCATCATCTACACCCGTGAGCAGTTGTTCGGTGGCAAGCAACTGACCGAGGAAATCCAGCGCCGTTATGGTCTGTCGATG
GGCGAGGCAGGGCTGGCCAAGAAGCAGGGCGGGCTGCCGGATGACTACGGCAGCGAGGTGCTGGAGCCCTTCAAGGACGC
CATGGTGCAGCAGGTTTCGCGCTCTTTGCAGTTCTTCTTTGCGGCCGGCCAGCACAACTCGGTGGACTACATCATGCTGG
CCGGCGGTTCGGCTTCGATTGCCGGTCTTGAGCACCTGATCCAGAAGCGCCTCGGCGTGCCGACCATGGTCGCCAACCCT
TTTGCCGATATGGCGCTGAGTTCCAAGGTCAATGCCGGGGCCCTGGCCAGCGATGCTCCAGCGTTGATGCTTGCCTGTGG
TCTGGCCATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A077LFT9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	55.367	100	0.554
pilM	Acinetobacter baumannii D1279779	55.085	100	0.551
comM	Acinetobacter baylyi ADP1	53.107	100	0.531
pilM	Legionella pneumophila strain ERS1305867	47.458	100	0.475