Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSCI_RS11285 Genome accession   NZ_AP014637
Coordinates   2460831..2462996 (+) Length   721 a.a.
NCBI ID   WP_045486527.1    Uniprot ID   A0A077LGD9
Organism   Pseudomonas sp. StFLB209     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2455831..2467996
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSCI_RS11265 (PSCI_2230) comM 2458015..2459079 (+) 1065 WP_045486514.1 pilus assembly protein PilM Machinery gene
  PSCI_RS11270 (PSCI_2231) - 2459079..2459639 (+) 561 WP_045486517.1 PilN domain-containing protein -
  PSCI_RS11275 (PSCI_2232) pilO 2459636..2460259 (+) 624 WP_045486520.1 type 4a pilus biogenesis protein PilO -
  PSCI_RS11280 (PSCI_2233) - 2460256..2460783 (+) 528 WP_045486523.1 pilus assembly protein PilP -
  PSCI_RS11285 (PSCI_2234) pilQ 2460831..2462996 (+) 2166 WP_045486527.1 type IV pilus secretin PilQ Machinery gene
  PSCI_RS11290 (PSCI_2235) aroK 2463001..2463519 (+) 519 WP_045494141.1 shikimate kinase AroK -
  PSCI_RS11295 (PSCI_2236) aroB 2463638..2464741 (+) 1104 WP_045486530.1 3-dehydroquinate synthase -
  PSCI_RS11300 (PSCI_2237) - 2464753..2466411 (+) 1659 WP_045486532.1 AAA family ATPase -

Sequence

Protein


Download         Length: 721 a.a.        Molecular weight: 77190.49 Da        Isoelectric Point: 5.0389

>NTDB_id=66444 PSCI_RS11285 WP_045486527.1 2460831..2462996(+) (pilQ) [Pseudomonas sp. StFLB209]
MTRILSIIGVSLWIAMVAPIVQAATLKTLDVATLPGDRIELKLAFDAPVAAPRGYTTEQPARIALDLPGVNTQLTNRSRD
LGTGNARSVVVVQANDRTRIIINLTTLSPYSTRVEGNNLFVVIGQGAAAAQASQPGTAQAAARGATPAASGAARPAAVAS
GVAIAPGARAVRNLDFQRGELGEGNVLIDLSSAGIAPDIQERNGKIFVDFAKTQLPDPLRVRLDVKDFATPVQFVTATTS
GDKTSISIEPSGAYDYSAYQTENRLTISVRPLTNQDLERRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASD
TVQGGITLRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADI
AKLFQSVTSAEAQVEERGSITVDERTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSR
TSGSGKWIGYGLDDNLTSASTSGGSNATTDVGSSGTSTAASNRNIPFVDLGAPDATAGFGIGFMTSNTLIDLELSAMEKS
GNGEIVSQPKVVTSDKETAKILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLG
VPPIRKNEVNAKVLISDGETIVIGGVFSNTQSKVVEKVPFLGDVPYVGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVG
R

Nucleotide


Download         Length: 2166 bp        

>NTDB_id=66444 PSCI_RS11285 WP_045486527.1 2460831..2462996(+) (pilQ) [Pseudomonas sp. StFLB209]
ATGACCAGGATTCTCTCGATTATCGGCGTGTCGCTATGGATTGCGATGGTCGCTCCGATAGTCCAGGCTGCCACGCTCAA
GACGCTCGATGTCGCCACGCTGCCCGGAGACCGTATCGAACTGAAACTGGCGTTCGACGCTCCGGTGGCTGCGCCTCGCG
GTTACACCACCGAGCAGCCGGCGCGCATTGCACTCGACCTGCCCGGGGTCAACACCCAGCTGACCAACCGCAGCCGCGAC
CTGGGGACCGGTAATGCCCGTAGCGTTGTCGTGGTGCAGGCCAACGACCGTACCCGAATCATCATCAACCTGACCACCCT
GTCGCCCTACAGCACCCGGGTTGAGGGCAATAACCTGTTCGTTGTCATCGGCCAGGGCGCCGCCGCGGCGCAAGCCTCCC
AGCCGGGCACGGCGCAAGCTGCAGCACGCGGCGCTACGCCCGCTGCGAGTGGCGCCGCCAGGCCTGCCGCAGTCGCCTCG
GGGGTGGCCATCGCGCCAGGGGCCAGGGCCGTACGCAATCTGGATTTTCAGCGTGGTGAGCTGGGTGAGGGCAATGTTCT
CATCGACCTGAGCAGCGCTGGCATCGCTCCGGATATTCAGGAGCGCAACGGTAAAATCTTTGTCGACTTCGCCAAGACCC
AGCTCCCTGACCCTTTGCGTGTACGGCTGGATGTCAAGGATTTCGCCACCCCTGTGCAGTTCGTCACGGCAACCACCTCG
GGTGACAAGACCAGTATCAGCATCGAGCCGTCCGGGGCCTATGACTATTCCGCCTATCAGACCGAAAACCGCCTGACCAT
CAGCGTCCGCCCTCTGACCAATCAGGATCTGGAGCGCCGTGCGGCGGACAAACCTGTGTATACCGGCGAGAAGCTGTCAC
TCAACTTCCAGGACATTGATGTGCGGTCGGTGCTGCAATTGATTGCCGACTTCACCAACCTCAACCTGGTCGCCAGCGAC
ACCGTACAGGGCGGCATTACCCTGCGTCTGCAGAATGTGCCCTGGGACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGG
CCTGGACAAGCGCAAGATCGGTAATGTGTTGCTGGTGGCGCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAACTGG
AGTCGCAAAAGCAGATCGCCGAGCTGGCACCGCTGCGTCGTGAGTTGTTGCAGGTCAACTACGCCAAGGCTGCGGATATT
GCCAAGCTGTTTCAGTCAGTGACCAGTGCCGAGGCTCAGGTCGAGGAACGTGGCTCGATCACCGTGGATGAGCGCACCAA
CAACATCATTGCCTATCAGACCCAAGAGCGTCTTGATGAGCTGCGGCGCATCGTTGCCCAACTGGACATTCCGGTGCGTC
AGGTGATGATCGAAGCGCGCATCGTCGAGGCCAACGTCGATTACGACAAGGCGCTGGGCGTTCGCTGGGGCGGCAGCCGG
ACCAGTGGCAGTGGCAAATGGATCGGCTACGGTCTGGATGACAACCTCACCTCCGCCAGCACCTCGGGAGGCAGTAACGC
CACTACCGATGTGGGTTCATCGGGTACGAGTACGGCTGCTTCGAACCGCAACATCCCGTTCGTCGATCTCGGCGCGCCGG
ATGCCACGGCGGGCTTCGGCATCGGCTTCATGACCAGCAACACCTTGATCGACCTCGAACTCAGTGCCATGGAAAAGAGC
GGTAACGGCGAGATCGTCTCCCAGCCCAAGGTTGTCACCTCTGACAAGGAAACCGCCAAGATCCTCAAAGGCACCGAAAT
TCCCTATCAGGAGTCCAGCTCCAGTGGCGCGACCACGGTGAGCTTCAAGGAGGCTTCGCTGTCACTGGAGGTCACCCCGC
AGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGATTACCTCAATGCCGTGCTGGGT
GTGCCGCCGATCAGGAAAAACGAGGTCAACGCCAAGGTGCTGATTTCCGATGGCGAGACTATTGTTATTGGCGGCGTGTT
TTCCAACACTCAAAGTAAAGTTGTAGAAAAAGTGCCATTCCTTGGCGATGTGCCGTATGTTGGCCGCCTTTTCCGGCGTG
ACGTGGTGTCTGATTCAAAATCCGAGCTTCTGGTATTTCTGACACCACGCATCATGAACAACCAGGCGATTGCTGTGGGT
CGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(556-712)

Secretin and TonB N terminus short domain (STN)

(315-361)

Bacterial type II/III secretion system short domain (Secretin_N)

(389-454)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A077LGD9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.099

100

0.724
  comQ Acinetobacter baylyi ADP1

41.433

98.752

0.409
  pilQ Acinetobacter baumannii strain A118

38.675

100

0.405
  pilQ Acinetobacter baumannii D1279779

38.675

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.714

97.087

0.366
  pilQ Legionella pneumophila strain ERS1305867

37.518

96.117

0.361

Multiple sequence alignment