Detailed information    

experimental Experimentally validated

Overview

Name   comL   Type   Machinery gene
Locus tag   NGFG_RS01495 Genome accession   NC_022240
Coordinates   279665..280468 (-) Length   267 a.a.
NCBI ID   WP_003687645.1    Uniprot ID   A0AA44ZGK5
Organism   Neisseria gonorrhoeae MS11     
Function   DNA processing; DNA transport into the cytoplasm   
DNA processing DNA binding and uptake

Function

A novel peptidoglycan-linked lipoprotein (ComL) has been identified which is required for efficient transformation of Neisseria gonorrhoeae by species-related DNA. Three competence factors unrelated to pilus biogenesis, ComA, ComL and Tpc, are not essential for DNA uptake and rather act in a subsequent step. The respective mutants, however, lack the characteristic nucleolytic processing observed with the incoming DNA in both wild-type and non-transformable RecA-deficient N. gonorrhoeae, indicating that they are blocked in the processing and/or the delivery of DNA to the cytoplasm.


Genomic Context

Location: 274665..285468
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS14965 - 277069..277344 (-) 276 WP_153707850.1 hypothetical protein -
  NGFG_RS01490 (NGFG_00417) comA 277376..279595 (+) 2220 WP_020997305.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NGFG_RS01495 (NGFG_00418) comL 279665..280468 (-) 804 WP_003687645.1 outer membrane protein assembly factor BamD Machinery gene
  NGFG_RS01500 (NGFG_00419) rluD 280467..281591 (+) 1125 WP_003687642.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  NGFG_RS01505 (NGFG_00420) - 281718..282692 (-) 975 Protein_281 bile acid:sodium symporter family protein -
  NGFG_RS15410 - 282769..282990 (-) 222 WP_229694461.1 hypothetical protein -
  NGFG_RS01515 (NGFG_00422) pgeF 283145..283924 (+) 780 WP_003687651.1 peptidoglycan editing factor PgeF -
  NGFG_RS01520 (NGFG_00423) lptE 283976..284455 (+) 480 WP_003687653.1 LPS assembly lipoprotein LptE -
  NGFG_RS01525 (NGFG_00424) holA 284457..285455 (+) 999 WP_003687654.1 DNA polymerase III subunit delta -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30838.90 Da        Isoelectric Point: 9.1654

>NTDB_id=1106 NGFG_RS01495 WP_003687645.1 279665..280468(-) (comL) [Neisseria gonorrhoeae MS11]
MKKILLTVSLGLALSACATQGTADKDAQITQDWSVEKLYAEAQDELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAY
AYYKDDEKDKALAAIERFRRLHPQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAKKIIGSYQNTRYVEESLAILELAYKKLDKPQLAADTRRV
LETNFPKSPFLTHAWQPDDMPWWRYWH

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1106 NGFG_RS01495 WP_003687645.1 279665..280468(-) (comL) [Neisseria gonorrhoeae MS11]
ATGAAAAAAATTCTTTTAACGGTTTCATTAGGTTTGGCACTGAGTGCCTGTGCCACTCAAGGTACGGCCGACAAAGATGC
CCAGATTACCCAAGATTGGAGTGTGGAGAAGCTCTATGCCGAAGCCCAGGACGAATTGAACAGCAGCAATTATACGCGGG
CTGTCAAGTTATACGAAATCTTGGAATCGCGCTTCCCCACCAGCCGCCATGCCCGGCAATCCCAACTGGATACCGCATAC
GCCTATTATAAAGACGATGAAAAAGACAAGGCTCTGGCGGCAATCGAACGCTTCCGCCGCCTCCATCCGCAGCATCCGAA
CATGGATTACGCGCTGTACCTGCGCGGCTTGGTGCTGTTTAACGAAGATCAGTCCTTCCTGAACAAACTGGCCTCGCAAG
ACTGGTCCGACCGCGACCCGAAAGCCAACCGCGAAGCGTACCAGGCGTTTGCGGAACTCGTCCAACGCTTCCCGAACAGC
AAATACGCCGCCGATGCAACCGCACGCATGGTCAAACTGGTCGATGCTTTGGGCGGCAATGAAATGTCGGTGGCGCGTTA
CTACATGAAACGCGGCGCATATATCGCCGCCGCCAACCGCGCCAAAAAAATTATCGGCAGCTATCAAAATACACGCTATG
TCGAAGAATCACTGGCCATATTGGAACTTGCCTACAAGAAGTTGGACAAGCCCCAGCTTGCCGCCGATACGCGCCGCGTA
TTGGAAACCAACTTCCCGAAAAGCCCGTTTTTGACGCACGCTTGGCAGCCCGACGATATGCCCTGGTGGCGTTACTGGCA
TTAA

Domains

Predicted by InterproScan.

Outer membrane lipoprotein (YfiO)

(34-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria meningitidis MC58

96.255

100

0.963

Multiple sequence alignment    



References

[1] M Fussenegger et al. (1996) A novel peptidoglycan-linked lipoprotein (ComL) that functions in natural transformation competence of Neisseria gonorrhoeae. Molecular Microbiology 19(5):1095-105. [PMID: 8830266]
[2] D Facius et al. (1996) Sequential action of factors involved in natural competence for transformation of Neisseria gonorrhoeae. FEMS Microbiology Letters 137(2-3):159-64. [PMID: 8998979]