Detailed information    

experimental Experimentally validated

Overview

Name   comL   Type   Machinery gene
Locus tag   NMB_RS03635 Genome accession   NC_003112
Coordinates   734869..735672 (-) Length   267 a.a.
NCBI ID   WP_002244053.1    Uniprot ID   Q9K0B1
Organism   Neisseria meningitidis MC58     
Function   DNA binding   
DNA binding and uptake

Function

The ComL DNA binding properties and outer membrane localization suggest that this lipoprotein plays a direct role in neisserial transformation, while neisserial SSB is a DNA binding protein that contributes to the terminal part of the transformation process. ComL was found to co-purify with the outer membrane, directly interacting with the secretin PilQ.


Genomic Context

Location: 729869..740672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS12120 - 732149..732340 (-) 192 Protein_692 hypothetical protein -
  NMB_RS03630 (NMB0702) comA 732422..734641 (+) 2220 WP_153063205.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  NMB_RS03635 (NMB0703) comL 734869..735672 (-) 804 WP_002244053.1 outer membrane protein assembly factor BamD Machinery gene
  NMB_RS03640 (NMB0704) rluD 735671..736795 (+) 1125 WP_002247551.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  NMB_RS03645 (NMB0705) - 736948..737895 (-) 948 WP_002244055.1 bile acid:sodium symporter family protein -
  NMB_RS12125 - 738115..738288 (-) 174 Protein_697 IS5/IS1182 family transposase -
  NMB_RS03650 (NMB0706) pgeF 738455..739234 (+) 780 WP_002244057.1 peptidoglycan editing factor PgeF -
  NMB_RS03655 (NMB0707) lptE 739286..739765 (+) 480 WP_002217671.1 LPS assembly lipoprotein LptE -

Sequence

Protein


Download         Length: 267 a.a.        Molecular weight: 30915.00 Da        Isoelectric Point: 9.3369

>NTDB_id=1102 NMB_RS03635 WP_002244053.1 734869..735672(-) (comL) [Neisseria meningitidis MC58]
MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQDELNSSNYTRAVKLYEILESRFPTSRHAQQSQLDTAY
AYYKDDEKDKALAAIDRFRRLHPQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESLAILELAYKKLDKPRLAADTRRV
LETNFPKSPFLKQPWRSDDMPWWRYWH

Nucleotide


Download         Length: 804 bp        

>NTDB_id=1102 NMB_RS03635 WP_002244053.1 734869..735672(-) (comL) [Neisseria meningitidis MC58]
ATGAAAAAAATTCTTTTAACGGTTTCATTAGGTTTGGCACTGAGTGCCTGTGCCACTCAAGGTACGGTAGATAAAGATGC
CCAGATTACCCAAGACTGGAGTGTGGAAAAACTCTATGCCGAAGCCCAGGACGAGCTGAACAGCAGCAATTATACGCGAG
CTGTCAAATTATACGAAATCTTGGAATCGCGCTTCCCTACCAGCCGCCATGCCCAGCAATCCCAACTGGATACGGCATAC
GCCTACTATAAAGATGATGAGAAGGATAAGGCGCTGGCCGCCATTGACCGCTTCCGCCGCCTTCACCCTCAACACCCGAA
TATGGACTACGCGCTATACCTGCGCGGTTTGGTACTGTTCAACGAAGATCAATCTTTCTTGAACAAGCTGGCCTCGCAAG
ACTGGTCCGACCGCGACCCGAAAGCCAACCGCGAAGCGTATCAGGCCTTTGCGGAACTCGTCCAACGCTTCCCGAACAGC
AAATACGCCGCCGATGCGACCGCACGCATGGTCAAACTGGTCGATGCTTTGGGCGGCAATGAAATGTCGGTAGCGCGTTA
CTACATGAAACGCGGCGCATATATCGCCGCCGCCAACCGCGCCCAAAAAATTATCGGCAGCTATCAAAATACACGCTATG
TCGAAGAATCGCTCGCCATATTGGAACTTGCCTACAAGAAGTTGGACAAGCCCCGGCTTGCCGCCGATACGCGCCGCGTG
TTGGAAACCAACTTCCCGAAAAGCCCGTTCTTAAAGCAGCCGTGGCGATCTGACGATATGCCCTGGTGGCGTTACTGGCA
CTGA

Domains

Predicted by InterproScan.

Outer membrane lipoprotein (YfiO)

(34-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9K0B1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comL Neisseria gonorrhoeae MS11

96.255

100

0.963

Multiple sequence alignment    



References

[1] Afsaneh V Benam et al. (2011) Structure-function relationships of the competence lipoprotein ComL and SSB in meningococcal transformation. Microbiology (Reading, England) 157(Pt 5):1329-1342. [PMID: 21330432]