Detailed information NTG0001128

Experimentally validated

Overview

• Name: comA
• Type: Machinery gene
• Locus tag: NGFG_RS01490
• Genome accession: NC_022240
• Coordinates: 277376..279595 (+)
• Length: 739 a.a.
• NCBI ID: WP_020997305.1
• Uniprot ID: -
• Organism: Neisseria gonorrhoeae MS11
• Function: DNA processing; DNA transport into the cytoplasm DNA processing DNA binding and uptake

Function

Using piliated and non-piliated comA mutants the consequences of transformation deficiency on pilin phase variation were assessed. Three competence factors unrelated to pilus biogenesis, ComA, ComL and Tpc, are not essential for DNA uptake and rather act in a subsequent step. The respective mutants, however, lack the characteristic nucleolytic processing observed with the incoming DNA in both wild-type and non-transformable RecA-deficient N. gonorrhoeae, indicating that they are blocked in the processing and/or the delivery of DNA to the cytoplasm.

Genomic Context

Location: 272376..284595

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NGFG_RS01485 (NGFG_00416)	-	272392..276990 (-)	4599	WP_003687639.1	S6 family peptidase	-
NGFG_RS14965	-	277069..277344 (-)	276	WP_153707850.1	hypothetical protein	-
NGFG_RS01490 (NGFG_00417)	comA	277376..279595 (+)	2220	WP_020997305.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
NGFG_RS01495 (NGFG_00418)	comL	279665..280468 (-)	804	WP_003687645.1	outer membrane protein assembly factor BamD	Machinery gene
NGFG_RS01500 (NGFG_00419)	rluD	280467..281591 (+)	1125	WP_003687642.1	23S rRNA pseudouridine(1911/1915/1917) synthase RluD	-
NGFG_RS01505 (NGFG_00420)	-	281718..282692 (-)	975	Protein_281	bile acid:sodium symporter family protein	-
NGFG_RS15410	-	282769..282990 (-)	222	WP_229694461.1	hypothetical protein	-
NGFG_RS01515 (NGFG_00422)	pgeF	283145..283924 (+)	780	WP_003687651.1	peptidoglycan editing factor PgeF	-
NGFG_RS01520 (NGFG_00423)	lptE	283976..284455 (+)	480	WP_003687653.1	LPS assembly lipoprotein LptE	-

Sequence

Protein

• Length: 739 a.a.
• Molecular weight: 79820.60 Da
• Isoelectric Point: 10.3677

>NTDB_id=1128 NGFG_RS01490 WP_020997305.1 277376..279595(+) (comA) [Neisseria gonorrhoeae MS11]
MFRRWFLPCWVVGVAVSFALSVVPHWPFWLAAFAVLAVLARRFAFAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVP
LTVEVTDMPRSDGRRVQFAAKAVDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGVGGV
GTVGADRVLLHGGSGWGIAVWRSRISRNWRQADADGGLSDGIGLMRALSVGEQSALRPGLWQAFRPLGLTHLVSISGLHV
TMVAVLFAWLAKRLLACSPRLPARPRAWVLAAGCAGALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALA
AVLLFDPLAVLGVGTWLSFGLVAALIWACAGRLYEGKRQTAVRGQWAASVLSLVLLGYLFASLPLVSPLVNAVSIPWFSW
VLTPLALLGSVVPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVAAAPLPLLVLAVCAALLLLLPRGLGLRPWAVLLLAG
FVSYRPEAVPENEAAVTVWDAGQGLSVLVRTANRHLLFDTGTVAAAQTGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQA
VGKIPNGGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLRPSERKNIDDNGKSCVLRVVAGGAALLVTGDLDTKGEESLVG
KYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYANAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGG
VKAQRLRVYKFYWQKKPFE

Nucleotide

• Length: 2220 bp

>NTDB_id=1128 NGFG_RS01490 WP_020997305.1 277376..279595(+) (comA) [Neisseria gonorrhoeae MS11]
GTGTTCCGTCGTTGGTTTTTGCCGTGTTGGGTTGTCGGCGTGGCAGTTTCGTTCGCACTGTCGGTTGTACCGCATTGGCC
GTTTTGGTTGGCGGCTTTTGCGGTTTTGGCTGTGCTTGCAAGGCGTTTTGCGTTTGCCGGTCTGATGCTGTGCGTGTTGG
CGGGCGCGGCTTACGGCGTATTCAGAACGGAAGCGGCACTGTCTTCGCAATGGCGGGCGGAGGCGGTTTCAGGCGTGCCT
TTGACGGTGGAAGTGACGGATATGCCGAGGTCGGACGGGCGGCGCGTGCAGTTTGCGGCAAAGGCTGTGGACAGCGGCGG
TCGGACGTTTGATTTGCTGCTGTCGGACTACAAACGGCGCGAATGGGCGGTCGGGAGCAGATGGCGGATAACGGCACGCG
TGCACCCCGTCGTCGGGGAGTTGAACCTGCGCGGTTTGAACCGTGAGGCGTGGGCATTATCCAACGGGGTAGGCGGCGTG
GGGACGGTCGGTGCGGACAGGGTTTTGCTGCATGGCGGAAGCGGTTGGGGGATTGCGGTTTGGCGCAGCCGCATCAGCCG
CAATTGGCGGCAGGCGGATGCGGACGGCGGGCTTTCAGACGGCATCGGGCTGATGCGCGCGTTGAGCGTGGGCGAACAGT
CGGCATTGCGCCCCGGATTGTGGCAGGCGTTCCGGCCGTTGGGGCTGACGCATTTGGTCAGTATTTCGGGTTTGCACGTT
ACGATGGTGGCGGTGCTGTTTGCGTGGCTGGCGAAGCGGCTGCTTGCCTGTTCCCCGCGCCTTCCCGCCCGGCCGCGCGC
GTGGGTTTTGGCGGCGGGGTGTGCGGGCGCGCTGTTTTACGCGCTGCTTGCCGGTTTTTCCGTGCCGACGCAGCGCAGTG
TTTTGATGTTGGCGGCGTTCGCGTGGGCGTGGCGCAGGGGAAGACTGTCGGCGTGGGCGACGTGGTGGCAGGCGTTGGCG
GCGGTGCTGCTGTTCGACCCTTTGGCGGTTTTGGGTGTCGGTACTTGGCTGTCTTTCGGTTTGGTGGCGGCCCTGATATG
GGCGTGTGCAGGGCGTTTGTACGAGGGGAAACGGCAAACCGCCGTGCGCGGGCAGTGGGCGGCTTCGGTGTTGTCTCTGG
TTTTGCTCGGTTATCTGTTTGCCTCGCTGCCTTTGGTCAGCCCTTTGGTCAATGCGGTGTCGATACCGTGGTTTTCTTGG
GTGTTGACCCCGCTGGCGTTGCTGGGTTCGGTCGTGCCGTTTGCGCCGCTTCAGCAGGCGGGGGCATTTTTGGCGGAATA
CACTTTGCGGTTTTTGGTGTGGCTTGCCGATGTGTCGCCTGAGTTTGCCGTTGCCGCCGCACCTTTGCCGCTGCTGGTGT
TGGCGGTGTGTGCCGCTTTGCTGTTGCTGCTGCCGCGCGGCTTGGGTTTGCGTCCGTGGGCGGTGTTGCTGTTGGCAGGG
TTTGTATCCTACCGTCCTGAAGCAGTACCTGAAAACGAAGCTGCGGTTACGGTTTGGGATGCGGGGCAGGGCTTGTCGGT
GCTTGTGCGGACGGCAAACCGCCATCTGCTTTTCGATACGGGAACGGTAGCGGCGGCACAAACGGGTATCGTGCCGAGTT
TGAATGCGGCGGGTGTCCGCCGTTTGGACAAGCTGGTTCTGTCGCATCACGACAGCGACCACGACGGCGGCTTTCAGGCA
GTCGGCAAGATACCGAACGGCGGGATTTATGCCGGACAGCCGGAATTTTATGAGGGGGCGCGGCATTGTGCGGAACAGCG
TTGGCAATGGGACGGCGTGGATTTCGAGTTTTTGAGGCCGTCTGAACGCAAAAATATCGATGATAATGGGAAAAGTTGTG
TTTTGCGTGTTGTGGCGGGCGGTGCGGCACTGCTGGTAACGGGAGATTTGGATACGAAGGGCGAGGAAAGCCTGGTCGGC
AAGTATGGAGGCAACCTGTACAGCCAGGTGTTGGTGTTGGGGCATCACGGCAGCAATACGTCCTCGTCGGGCGTGTTCCT
CAATGCCGTTTCGCCCGAATATGCCGTTGCTTCAAGCGGTTATGCCAACGCCTACAAGCATCCGACCGAAGCGGTGCAGA
ACCGTGTCCGCGCACACGGCATCAAGTTGTTGCGGACGGATTTGTCGGGCGCGCTGCAATTCGGCTTGGGACGCGGAGGC
GTAAAGGCTCAACGTTTGAGAGTGTATAAATTCTATTGGCAGAAAAAACCGTTTGAGTGA

Domains

Predicted by InterproScan.

• Competence protein (Competence) (208-465)

• Metallo-beta-lactamase superfamily (Lactamase_B) (500-677)

• Domain of unknown function (DUF4131) (DUF4131) (7-167)

References

• [1]: D Facius et al. (1996) Sequential action of factors involved in natural competence for transformation of Neisseria gonorrhoeae. FEMS Microbiology Letters 137(2-3):159-64. [PMID: 8998979]
• [2]: D Facius et al. (1993) A novel determinant (comA) essential for natural transformation competence in Neisseria gonorrhoeae and the effect of a comA defect on pilin variation. Molecular Microbiology 10(4):699-712. [PMID: 7934834]