Detailed information NTG0663539

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: N023_RS14440
• Genome accession: NZ_CP093321
• Coordinates: 3369092..3370261 (-)
• Length: 389 a.a.
• NCBI ID: WP_003410864.1
• Uniprot ID: A0A0P9MZZ6
• Organism: Pseudomonas syringae CC440
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3364092..3375261

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N023_RS14425 (N023_14425)	-	3365379..3366440 (+)	1062	WP_024665837.1	microcin C ABC transporter permease YejB	-
N023_RS14430 (N023_14430)	-	3366440..3367462 (+)	1023	WP_003318547.1	ABC transporter permease	-
N023_RS14435 (N023_14435)	-	3367464..3369041 (+)	1578	WP_024665838.1	ABC transporter ATP-binding protein	-
N023_RS14440 (N023_14440)	pilU	3369092..3370261 (-)	1170	WP_003410864.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
N023_RS14445 (N023_14445)	-	3370385..3370660 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
N023_RS14450 (N023_14450)	pgm	3370735..3372381 (-)	1647	WP_024665839.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
N023_RS14455 (N023_14455)	-	3372545..3374872 (-)	2328	WP_024665840.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42663.74 Da
• Isoelectric Point: 5.8676

>NTDB_id=663539 N023_RS14440 WP_003410864.1 3369092..3370261(-) (pilU) [Pseudomonas syringae CC440]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGTLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=663539 N023_RS14440 WP_003410864.1 3369092..3370261(-) (pilU) [Pseudomonas syringae CC440]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCACCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TTATGGACGAGGAGCAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCTATGGCGGGTATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAGAAGCGCGGTCTGGTGTTGTTTGTCGGGGCCACCGGTT
CCGGGAAATCGACCTCTTTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGTGGCCATATCATCACCATTGAAGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACTCGCAGCTTCCATGC
GGCACTGAAGAACACCCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCTGACACCGGCCACCTGGCGATCTCGACACTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
ACTGGTCAGAACCCCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACACGGCGCTA
TTTAATCTGGCCGTTGAAGGTGCAATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGTGCCGGGACCCTGACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGAGCCGGGTCCGCAGGCCTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

3D structure

• AlphaFold DB: A0A0P9MZZ6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.331	99.486	0.56
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	50.829	93.059	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.903	98.458	0.383
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362