Detailed information NTG0661166

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MME58_RS14865
• Genome accession: NZ_CP092923
• Coordinates: 3352543..3353712 (-)
• Length: 389 a.a.
• NCBI ID: WP_054987495.1
• Uniprot ID: A0A0Q0HIQ9
• Organism: Pseudomonas syringae pv. tagetis strain ICMP 4091
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3347543..3358712

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MME58_RS14850 (MME58_14850)	-	3348769..3349833 (+)	1065	WP_055004969.1	microcin C ABC transporter permease YejB	-
MME58_RS14855 (MME58_14855)	-	3349833..3350855 (+)	1023	WP_055004968.1	ABC transporter permease	-
MME58_RS14860 (MME58_14860)	-	3350857..3352434 (+)	1578	WP_055004967.1	ABC transporter ATP-binding protein	-
MME58_RS14865 (MME58_14865)	pilU	3352543..3353712 (-)	1170	WP_054987495.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MME58_RS14870 (MME58_14870)	-	3353858..3354133 (+)	276	WP_055004966.1	peptidylprolyl isomerase	-
MME58_RS14875 (MME58_14875)	pgm	3354207..3355853 (-)	1647	WP_055004965.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
MME58_RS14880 (MME58_14880)	-	3355968..3358295 (-)	2328	WP_055004964.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42696.83 Da
• Isoelectric Point: 5.6672

>NTDB_id=661166 MME58_RS14865 WP_054987495.1 3352543..3353712(-) (pilU) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEEGVASLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=661166 MME58_RS14865 WP_054987495.1 3352543..3353712(-) (pilU) [Pseudomonas syringae pv. tagetis strain ICMP 4091]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGGGTGCTGAAGCCGCTGGGCAGTGAAACCCTCAAGCCCGGCGAGGTGGCGGTGATTGCCCAGGGAC
TGATGGACGAAGAGCAGAAGCTTGAGTTCCTGCGTGAGCTGGAGATGAACCTTGCCGTTTCGCTGGCAGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTATCCATCGTCGCCCGGAATATCAAGCTGGACATTCCGCGTTTCGA
GGACTTGTTCCTGCCGCCCGTACTGCTCGACGTGATTATGGAAAAACACGGTCTGGTGCTGTTCGTCGGTGCCACCGGCT
CAGGCAAATCGACTTCACTGGCGGCGCTGATCGACTATCGCAACCGCAACGCCAGTGGTCATATCATTACCATCGAGGAT
CCGGTGGAATTCATCCATCGCCACAAGAAGTCCATCGTCAACCAGCGTGAGGTGGGCGTCGATACGCGAAGCTTCCGCGC
TGCACTGAAGAACACGCTGCGTCAGGCTCCGGATGTCATTCTGATCGGCGAGATCCGTGATCGCGAGACCATGGAGCACG
CACTGGCGTTTGCCGACACCGGTCATCTGGCGATTTCGACATTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAGGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCTCAGCG
GCTGGTCAGAACCCCGGACGGCAAACGCCGTGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATCCGCGACCTGATTC
AGCGCAACGAACTGACCGAGCTCAAGGGCATCATGGAGAAGTCCGGCAGCCTTGGTATGCAGACGTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAAGGTGCAATCAGTGAAGAGGAGGCTCTGAAAAACGCCGATTCGCAGAATAACGTGCGCCTGCG
CCTCAAACTGCACAGCGAGGAGGGAGTGGCCAGCCTGACGACACCGCCGCCGGCACCCACCGGCAGCAGTACGGCGAGCA
CCGCCGAGTGGGGTTTGGTCGATGACGACGAGCCTGGCCCACAGGCTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

3D structure

• AlphaFold DB: A0A0Q0HIQ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362