Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   M3B_RS02740 Genome accession   NZ_AP014596
Coordinates   525759..527657 (+) Length   632 a.a.
NCBI ID   WP_002994648.1    Uniprot ID   A0A5S4TL28
Organism   Streptococcus pyogenes strain M3-b     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 520759..532657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3B_RS02725 (M3B_0557) fmt 522737..523672 (+) 936 WP_011054834.1 methionyl-tRNA formyltransferase -
  M3B_RS02730 (M3B_0558) rsmB 523662..524984 (+) 1323 WP_002983657.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  M3B_RS02735 (M3B_0559) - 525022..525762 (+) 741 WP_002983660.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  M3B_RS02740 (M3B_0560) pknB 525759..527657 (+) 1899 WP_002994648.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  M3B_RS02745 (M3B_0561) liaF 527780..528472 (+) 693 WP_011054833.1 cell wall-active antibiotics response protein LiaF -
  M3B_RS02750 (M3B_0562) - 528469..529473 (+) 1005 WP_002991890.1 sensor histidine kinase -
  M3B_RS02755 (M3B_0563) - 529466..530107 (+) 642 WP_002983671.1 response regulator transcription factor -
  M3B_RS02760 (M3B_0564) - 530144..531544 (+) 1401 WP_011054832.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  M3B_RS02765 (M3B_0565) - 531544..531921 (+) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -

Sequence

Protein


Download         Length: 632 a.a.        Molecular weight: 68819.06 Da        Isoelectric Point: 7.2899

>NTDB_id=66095 M3B_RS02740 WP_002994648.1 525759..527657(+) (pknB) [Streptococcus pyogenes strain M3-b]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGADLKRYIQNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIEENHNVP
QALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYNRSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESR
LDQTNQTDALQPPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDVGLKVGKIR
QIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDYQEAMNSLIETYGVPKSKIKIERIVTNEYPE
NTVISQSPSAGDKFNPNGKSKITLSVAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSS
KAIVSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSPSETTAQTP

Nucleotide


Download         Length: 1899 bp        

>NTDB_id=66095 M3B_RS02740 WP_002994648.1 525759..527657(+) (pknB) [Streptococcus pyogenes strain M3-b]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGCATTCTGAAATCTATTGGCCGCGGTGGTATGGCGGATGTTTA
TTTAGCAAATGATTTGATCTTAGATAATGAAGACGTTGCAATCAAGGTCTTGCGTACCAATTATCAAACAGATCAGGTAG
CAGTTGCGCGTTTCCAACGAGAAGCGCGAGCCATGGCTGAATTGAACCATCCCAATATTGTTGCCATCCGGGATATAGGT
GAAGAAGACGGACAGCAATTTTTAGTAATGGAATATGTGGATGGTGCTGATCTAAAGAGATACATTCAAAATCATGCTCC
ATTATCTAATAATGAAGTGGTTAGAATTATGGAAGAAGTCCTTTCTGCTATGACTTTAGCCCACCAAAAAGGAATTGTAC
ACAGAGATTTAAAACCTCAAAATATCCTACTAACTAAGGAGGGTGTTGTCAAAGTAACTGATTTCGGCATCGCAGTAGCC
TTTGCAGAAACAAGCTTGACACAAACTAATTCGATGTTAGGCAGTGTTCATTACTTGTCTCCAGAACAGGCTCGCGGCTC
CAAAGCTACGATTCAAAGTGATATTTATGCGATGGGGATTATGCTCTTTGAGATGTTGACAGGCCATATCCCTTATGATG
GCGATAGTGCTGTTACGATTGCCTTGCAACATTTTCAAAAGCCTCTTCCATCTATTATCGAGGAGAACCACAATGTGCCA
CAAGCTTTGGAGAATGTTGTTATTCGAGCAACAGCCAAGAAATTAAGTGATCGTTACGGATCAACCTTTGAAATGAGTCG
TGACTTAATGACGGCGCTTAGTTATAATCGTAGTCGGGAGCGTAAGATTGTCTTTGAGGATGTTGAAAGTACCAAACCCC
TCCCCAAAGTGGCCTCAGGTCCCACCGCTTCTGTAAAATTGTCTCCCCCTACCCCAACAGTGTTAACACAGGAAAGTCGA
TTGGATCAAACTAATCAAACAGATGCTTTACAGCCCCCCACCAAAAAGAAAAAAAGTGGTCGTTTTTTAGGTACTTTATT
CAAAATTCTTTTTTCTTTCTTTATTGTAGGTGTAGCACTCTTTACTTATCTTATACTAACTAAACCAACTTCTGTGAAAG
TGCCTAATGTAGCAGGCACTAGTCTTAAAGTTGCCAAACAAGAACTGTATGATGTTGGGCTAAAAGTGGGTAAAATCAGG
CAAATTGAGAGTGATACGGTTGCTGAGGGAAATGTAGTTAGAACAGATCCTAAAGCAGGAACAGCTAAGAGGCAAGGCTC
AAGCATTACGCTTTATGTGTCAATTGGAAACAAAGGTTTTGACATGGAAAACTACAAAGGACTAGATTATCAAGAAGCTA
TGAATAGTTTGATAGAAACTTATGGTGTTCCAAAATCAAAAATCAAAATTGAGCGCATTGTAACTAATGAATATCCTGAA
AATACAGTCATCAGTCAATCGCCAAGTGCGGGTGATAAATTTAATCCAAACGGAAAGTCTAAAATTACGCTCAGTGTTGC
TGTTAGTGATACGATCACTATGCCTATGGTAACAGAATATAGTTATGCAGATGCAGTCAATACCTTAACAGCTTTAGGTA
TAGATGCATCTAGAATAAAAGCTTATGTGCCAAGCTCTAGCTCAGCAACGGGCTTTGTGCCAATTCATTCTCCTAGTTCT
AAAGCTATTGTCAGTGGTCAATCTCCTTACTATGGAACGTCTTTGAGTCTGTCTGATAAAGGAGAGATTAGTCTTTACCT
TTATCCAGAAGAAACACACTCTTCTAGTAGCTCATCGAGTTCAACGTCAAGTTCAAACAGTTCTTCAACAAATGATAGTA
CTGCACCAGGTAGCAACACTGAATTAAGCCCATCAGAAACTACTGCTCAAACACCTTAA

Domains

Predicted by InterproScan.

Protein kinase domain (Pkinase)

(12-242)

PASTA domain (PASTA)

(372-434)

PASTA domain (PASTA)

(441-506)

PASTA domain (PASTA)

(512-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TL28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.424

93.354

0.611
  stkP/pknB Streptococcus salivarius strain HSISS4

61.513

94.146

0.579
  stkP Streptococcus pneumoniae TIGR4

57.171

81.646

0.467
  stkP Streptococcus pneumoniae D39

56.977

81.646

0.465
  stkP Streptococcus pneumoniae R6

56.977

81.646

0.465
  stkP Streptococcus pneumoniae Rx1

56.783

81.646

0.464

Multiple sequence alignment