Detailed information NTG0660214

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MK616_RS12685
• Genome accession: NZ_CP092778
• Coordinates: 2605221..2605835 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus amyloliquefaciens strain WF2020
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2600221..2610835

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MK616_RS12655 (MK616_12655)	rpsT	2600524..2600790 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MK616_RS12660 (MK616_12660)	holA	2600753..2601848 (-)	1096	Protein_2451	DNA polymerase III subunit delta	-
MK616_RS12665 (MK616_12665)	-	2601888..2602039 (-)	152	Protein_2452	hypothetical protein	-
MK616_RS12670 (MK616_12670)	-	2602080..2602214 (+)	135	WP_003152870.1	YqzM family protein	-
MK616_RS12675 (MK616_12675)	comEC	2602233..2604584 (-)	2352	WP_029973403.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MK616_RS12680 (MK616_12680)	-	2604585..2605154 (-)	570	WP_003152868.1	ComE operon protein 2	-
MK616_RS12685 (MK616_12685)	comEA	2605221..2605835 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
MK616_RS12690 (MK616_12690)	comER	2605894..2606715 (+)	822	WP_202331466.1	late competence protein ComER	-
MK616_RS12695 (MK616_12695)	-	2606784..2607521 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
MK616_RS12700 (MK616_12700)	rsfS	2607518..2607874 (-)	357	WP_007408260.1	ribosome silencing factor	-
MK616_RS12705 (MK616_12705)	yqeK	2607892..2608452 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MK616_RS12710 (MK616_12710)	-	2608442..2609011 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
MK616_RS12715 (MK616_12715)	yhbY	2609022..2609312 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
MK616_RS12720 (MK616_12720)	aroE	2609306..2610148 (-)	843	WP_240680064.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=660214 MK616_RS12685 WP_014418419.1 2605221..2605835(-) (comEA) [Bacillus amyloliquefaciens strain WF2020]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=660214 MK616_RS12685 WP_014418419.1 2605221..2605835(-) (comEA) [Bacillus amyloliquefaciens strain WF2020]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363