Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MJ608_RS22345 Genome accession   NZ_CP092733
Coordinates   4813012..4814754 (-) Length   580 a.a.
NCBI ID   WP_039049614.1    Uniprot ID   -
Organism   Comamonas thiooxydans strain CHJ01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4808012..4819754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ608_RS22315 (MJ608_22285) - 4808527..4808997 (+) 471 WP_052052682.1 NUDIX domain-containing protein -
  MJ608_RS22320 (MJ608_22290) - 4809115..4809324 (-) 210 WP_039049611.1 DNA gyrase inhibitor YacG -
  MJ608_RS22325 (MJ608_22295) zapD 4809428..4810183 (-) 756 WP_039049612.1 cell division protein ZapD -
  MJ608_RS22330 (MJ608_22300) coaE 4810271..4810897 (-) 627 WP_229228151.1 dephospho-CoA kinase -
  MJ608_RS22335 (MJ608_22305) - 4810901..4811770 (-) 870 WP_003062192.1 A24 family peptidase -
  MJ608_RS22340 (MJ608_22310) pilC 4811770..4812987 (-) 1218 WP_003062193.1 type II secretion system F family protein Machinery gene
  MJ608_RS22345 (MJ608_22315) pilB 4813012..4814754 (-) 1743 WP_039049614.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MJ608_RS22355 (MJ608_22325) - 4815008..4815937 (-) 930 WP_003062198.1 polyprenyl synthetase family protein -
  MJ608_RS22360 (MJ608_22330) rplU 4816282..4816593 (+) 312 WP_003058536.1 50S ribosomal protein L21 -
  MJ608_RS22365 (MJ608_22335) rpmA 4816610..4816867 (+) 258 WP_003058534.1 50S ribosomal protein L27 -
  MJ608_RS22370 (MJ608_22340) cgtA 4817024..4818139 (+) 1116 WP_039049615.1 Obg family GTPase CgtA -
  MJ608_RS22375 (MJ608_22345) proB 4818250..4819389 (+) 1140 WP_012837105.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63074.28 Da        Isoelectric Point: 5.1537

>NTDB_id=659920 MJ608_RS22345 WP_039049614.1 4813012..4814754(-) (pilB) [Comamonas thiooxydans strain CHJ01]
MAAADTLQKNTPAPIAIPGLGRALISAGKVSQQAAENAAKKAATGKTPFITELSQSGEISALEIAETISMMFSTPLLDLN
AIDAQQLPRELLDPKISNAYRVLALSKRGNRLTIATADPTQQEAAEQIKFTTQLLVDWVVVEADKLQKLVEQTGKSVSES
LDSYSSSGDFDFDDVKIEDAPEEKDNAVGAEVEDAPVVKFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIAS
PPIAIKDKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEE
KERLLKAINRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINMPGVNQVNVNEKAGLTFAAALKSFLRQ
DPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCAQCKEPAD
IPHDALIDAGYDEAELDGSWVSYKPVGCPACNNGYKGRVGIYQVMPISEEIQRIILRDGSALEIAAQAKAEGVRSLRGSG
LHKVKLGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=659920 MJ608_RS22345 WP_039049614.1 4813012..4814754(-) (pilB) [Comamonas thiooxydans strain CHJ01]
ATGGCTGCTGCCGACACTCTGCAAAAGAACACTCCTGCGCCCATTGCCATCCCAGGTTTAGGCAGGGCCCTGATTTCTGC
AGGAAAGGTTTCGCAGCAAGCCGCCGAGAACGCAGCCAAGAAAGCCGCCACAGGCAAGACCCCGTTCATCACCGAGCTCA
GCCAGTCTGGTGAAATCAGCGCACTGGAAATTGCCGAAACCATTTCCATGATGTTCAGCACTCCGCTGCTGGACCTCAAC
GCCATCGACGCCCAGCAACTTCCGCGCGAGCTGCTGGACCCGAAAATCAGCAACGCCTATCGCGTGCTGGCACTCAGCAA
GCGCGGCAATCGGCTCACCATCGCCACGGCAGACCCTACTCAGCAGGAAGCAGCCGAGCAGATCAAGTTCACGACTCAGT
TACTGGTGGACTGGGTGGTTGTCGAGGCCGACAAGCTGCAAAAGCTCGTCGAGCAGACCGGCAAGAGCGTCAGCGAATCC
CTGGACTCCTATTCCAGCTCAGGGGACTTCGACTTTGACGACGTCAAGATCGAGGATGCCCCCGAGGAAAAAGACAATGC
CGTCGGCGCCGAGGTCGAGGATGCGCCGGTCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCCTTCAATATGCGCGCGT
CCGACTTGCACTTCGAGCCCTACGAACACAATTACCGCGTGCGCTTTCGCATCGACGGCGAACTGCGCGAGATTGCCTCT
CCTCCTATCGCGATCAAGGACAAGCTGGCTTCGCGCATCAAGGTGATCTCGCGCCTGGACATCTCGGAGAAACGCGTTCC
GCAAGACGGCCGCATGAAGCTCAAGGTCGGCCCCGATCGCGTCATCGACTTTCGTGTCAGCACCTTGCCCACGCTGTTTG
GCGAAAAGATCGTGATCCGTATTCTGGACCCGAGCTCGGCCAAGATGGGCATCGATGCCCTCGGCTACGAGCCCGAGGAG
AAAGAGCGCCTGCTCAAGGCCATCAACCGCCCCTACGGCATGATTCTGGTCACCGGCCCCACGGGCTCGGGCAAGACCGT
GTCGCTGTACACCTGCCTGAACCTGCTCAACCAGCCGGGCGTGAACATTGCCACCGCCGAAGACCCTTCCGAAATCAACA
TGCCCGGCGTCAACCAGGTAAATGTGAACGAAAAGGCCGGCCTGACCTTTGCTGCTGCGCTCAAGTCCTTTCTGCGCCAG
GATCCGGACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGACATCTCGATCAAGGCTGCCCAGACCGGGCA
CCTGGTGCTGTCCACGCTGCACACCAATGACGCTCCGACCACGCTGACGCGCATGCGCAATATGGGCATCGCCCCTTTCA
ATATTGCCTCCAGCGTGATTCTGATCACCGCCCAGCGTCTGGCGCGACGCCTGTGCGCGCAGTGCAAGGAACCCGCCGAC
ATACCGCACGACGCCCTGATCGATGCCGGCTACGACGAAGCCGAACTCGACGGCAGCTGGGTCAGCTACAAGCCTGTCGG
CTGCCCTGCCTGCAACAACGGCTACAAGGGACGTGTCGGTATTTATCAGGTGATGCCCATCAGCGAAGAGATTCAGCGCA
TCATCCTGCGTGACGGCAGCGCCCTGGAGATTGCCGCACAGGCCAAGGCCGAAGGAGTACGCTCTCTGCGCGGCTCGGGC
CTGCATAAAGTCAAGCTGGGTCTGACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(203-470)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.53

98.966

0.54
  pilB Acinetobacter baumannii D1279779

55.203

97.759

0.54
  pilF Neisseria gonorrhoeae MS11

52.473

97.586

0.512
  pilB Legionella pneumophila strain ERS1305867

50.883

97.586

0.497
  pilB Vibrio cholerae strain A1552

46.898

94.483

0.443
  pilB Vibrio campbellii strain DS40M4

44.029

96.724

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

46.008

90.69

0.417
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.421

98.276

0.378
  pilF Thermus thermophilus HB27

37.564

100

0.378