Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MJ595_RS08390 Genome accession   NZ_CP092489
Coordinates   1902714..1904405 (-) Length   563 a.a.
NCBI ID   WP_263082002.1    Uniprot ID   -
Organism   Endozoicomonas sp. Mp262     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1897714..1909405
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ595_RS08370 dauA 1897756..1899504 (+) 1749 WP_263081997.1 C4-dicarboxylic acid transporter DauA -
  MJ595_RS08375 - 1899652..1900092 (+) 441 WP_263081998.1 hypothetical protein -
  MJ595_RS08380 - 1900134..1900811 (-) 678 WP_263082000.1 class I SAM-dependent methyltransferase -
  MJ595_RS08385 - 1901073..1902437 (+) 1365 WP_263082001.1 hypothetical protein -
  MJ595_RS08390 pilB 1902714..1904405 (-) 1692 WP_263082002.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MJ595_RS08395 - 1904637..1904774 (+) 138 Protein_1635 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MJ595_RS08400 - 1905195..1906874 (-) 1680 WP_263082003.1 hypothetical protein -
  MJ595_RS08405 - 1907107..1908351 (-) 1245 WP_263082004.1 BamA/TamA family outer membrane protein -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62562.75 Da        Isoelectric Point: 5.0402

>NTDB_id=658050 MJ595_RS08390 WP_263082002.1 1902714..1904405(-) (pilB) [Endozoicomonas sp. Mp262]
MSDKPLTGLAARLVAEKLITEKKMRSAITATHSNKTSLPYYLFSENLIPARELAEATGREFGLPVIDINSMDVDNMPIDT
VPLKLVRRNQIIPLMNRGNRLFVAVTDPTEEKALDEFRFNTGLVIQPVIAEEGKLEIFIDKILDNPDNALSNEDDGCTSL
ENLDGETNEEVEDEDEADAPIVKFVNKMLLSAIKGGSSDLHFEPYEKAYRVRFRTDGVLHEVTRPPIELSQKISSRLKIM
SQMDISERRVPQDGRIKLKLSKNRAIDFRVNTCPTLWGEKIVLRILDPSSAKMGIDMLGYEEDQKAMYIEALEQPQGMIL
VTGPTGSGKTVSLYTGLNILNTPERNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDIIMVGEIRDLETA
NIAIKAAQTGHMVMSTLHTNSAPETLTRLQNMGVPSFNIATSVSLIIAQRLARRLCNHCKEWQDIPKEALKKEGFTDEQL
RGLKVYGPAGCDHCNQGYKGRVGIYEVVKMTPELQKVVMEEGNSLEIAGIAKKAGFNNLRESGLLKVMQGVTSLAEVNRV
TAE

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=658050 MJ595_RS08390 WP_263082002.1 1902714..1904405(-) (pilB) [Endozoicomonas sp. Mp262]
ATGTCAGATAAACCTCTAACAGGACTTGCCGCCAGACTTGTTGCAGAAAAGCTGATCACAGAAAAGAAAATGCGTTCAGC
CATCACCGCAACACACTCTAATAAAACCTCGTTACCCTATTACCTGTTCTCGGAAAACCTGATTCCTGCCCGGGAGCTTG
CTGAAGCAACAGGAAGGGAATTTGGCCTGCCCGTTATCGATATCAACTCAATGGATGTGGATAACATGCCCATAGATACC
GTTCCACTAAAACTTGTACGAAGGAACCAGATAATCCCGCTAATGAATCGGGGAAACCGCTTATTTGTAGCCGTCACAGA
TCCCACTGAAGAAAAAGCCCTGGATGAATTCAGGTTTAATACCGGACTTGTGATACAGCCGGTAATTGCCGAAGAGGGCA
AACTTGAAATCTTTATCGATAAAATACTGGATAACCCGGATAACGCCCTTTCCAATGAAGACGATGGCTGTACCAGTCTG
GAAAACCTTGACGGTGAGACAAACGAGGAAGTCGAGGACGAAGACGAAGCTGATGCCCCTATTGTCAAGTTTGTTAATAA
AATGCTGTTATCTGCCATAAAAGGGGGCTCATCGGACCTGCATTTTGAGCCTTATGAAAAGGCATATCGGGTCAGGTTCC
GCACCGATGGTGTCCTCCATGAAGTGACTCGTCCTCCCATAGAGCTCTCGCAAAAAATATCCTCCCGCCTGAAAATCATG
TCACAAATGGACATCTCTGAACGACGGGTTCCTCAAGACGGCCGGATAAAACTGAAGCTTTCAAAAAACAGAGCCATTGA
TTTTCGGGTCAATACCTGCCCAACCCTGTGGGGTGAAAAAATTGTGTTACGGATACTGGACCCCTCCAGTGCCAAGATGG
GTATTGATATGCTGGGTTATGAAGAAGACCAAAAAGCCATGTATATCGAAGCACTGGAGCAACCCCAGGGCATGATCCTG
GTGACAGGCCCAACCGGCTCCGGTAAAACCGTCTCTCTTTATACCGGCCTGAATATCCTTAACACTCCCGAGCGCAATAT
CTCTACCGCCGAAGATCCCGTTGAAATCAACCTGGAAGGCATCAATCAGGTCAATGTAAATCCCAAGCAGGGTATGGATT
TCGCCCAGGCCCTTCGCGCTTTCCTTCGTCAGGACCCGGATATTATTATGGTTGGGGAAATACGTGACCTGGAAACCGCC
AATATCGCCATCAAGGCTGCACAAACCGGCCATATGGTCATGTCCACCCTGCACACCAACAGCGCACCGGAAACACTGAC
CCGCCTCCAGAATATGGGTGTCCCCTCCTTTAATATCGCTACCTCAGTGAGCCTGATCATCGCCCAGCGTCTGGCTCGAA
GACTCTGCAACCATTGCAAAGAGTGGCAGGACATCCCCAAAGAGGCATTAAAAAAAGAGGGTTTTACAGATGAGCAACTC
CGGGGACTAAAAGTCTATGGTCCTGCTGGGTGTGATCACTGTAACCAGGGCTATAAAGGCCGGGTTGGCATTTACGAAGT
GGTGAAAATGACCCCGGAACTTCAAAAGGTCGTGATGGAAGAGGGCAACTCCCTGGAAATTGCCGGGATCGCGAAAAAAG
CAGGATTTAACAACCTGCGGGAGTCTGGTCTTTTAAAAGTCATGCAAGGGGTCACCAGCCTGGCTGAGGTTAACAGGGTG
ACAGCAGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-454)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.057

100

0.622
  pilB Acinetobacter baylyi ADP1

57.923

100

0.584
  pilB Legionella pneumophila strain ERS1305867

53.274

100

0.535
  pilB Vibrio cholerae strain A1552

50.357

99.467

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

49.468

100

0.496
  pilB Vibrio campbellii strain DS40M4

48.404

100

0.485
  pilF Neisseria gonorrhoeae MS11

48.022

98.757

0.474
  pilB/pilB1 Synechocystis sp. PCC 6803

35.961

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.109

92.895

0.382