Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MJO57_RS04195 Genome accession   NZ_CP092417
Coordinates   996869..998584 (-) Length   571 a.a.
NCBI ID   WP_252023208.1    Uniprot ID   -
Organism   Endozoicomonas sp. SCSIO W0465     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 991869..1003584
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO57_RS04185 (MJO57_04165) - 992666..993196 (-) 531 WP_252023204.1 hypothetical protein -
  MJO57_RS04190 (MJO57_04170) putA 993497..996736 (+) 3240 WP_252023206.1 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA -
  MJO57_RS04195 (MJO57_04175) pilB 996869..998584 (-) 1716 WP_252023208.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MJO57_RS04200 (MJO57_04180) - 998821..999303 (-) 483 WP_252026893.1 IS1595 family transposase -
  MJO57_RS04205 (MJO57_04185) - 999272..999760 (-) 489 WP_252018121.1 transposase -
  MJO57_RS04210 (MJO57_04190) - 999933..1000214 (-) 282 Protein_839 ABC transporter permease subunit -
  MJO57_RS04215 (MJO57_04195) - 1001385..1001918 (+) 534 WP_252023210.1 transposase -
  MJO57_RS04220 (MJO57_04200) - 1002124..1002444 (-) 321 WP_252023212.1 hypothetical protein -
  MJO57_RS04230 (MJO57_04210) - 1002847..1003272 (+) 426 WP_371924861.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62442.53 Da        Isoelectric Point: 5.7097

>NTDB_id=657286 MJO57_RS04195 WP_252023208.1 996869..998584(-) (pilB) [Endozoicomonas sp. SCSIO W0465]
MANKPLTGLAARLLADKAMEEAALHNAEAAAAKANKPLARYLLEKKLVDGKALILAIAEEFGLPVFDLSAFDKEHLVKEV
VEEKLIRKHNVLPLIKRGSHLFVAVSDPSNLQSLDEIRFNTGLTIHTVLSEPDRLEKMIDAALDDTSGAFSDLGDDGLVD
LEDLEVSAIDDGANDAQGTIGGGEDDTPVVKFVNKMLLSAIKGGASDLHFEPYERAYRVRFRTDGVLHEVSRPPVTLGSR
IASRLKIMSAMDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIDALGYEAEQKEMYLNAL
HQPQGMILVTGPTGSGKTVSLYTGLNILNTNERNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVG
EIRDLETANIAIKAAQTGHMVMSTLHTNSAAETLTRLQNMGVPSFNIATSVSLIIAQRLARRLCNHCKVKVQIPKETLLE
QGFTEEQADSAEIFGPKGCENCKSGYKGRVGIYEVVSITPGMQQIIMAEGNSLEISKQAQKEGFNNLRQSGLLKALQGVT
SLEEVNRVTQD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=657286 MJO57_RS04195 WP_252023208.1 996869..998584(-) (pilB) [Endozoicomonas sp. SCSIO W0465]
ATGGCAAACAAACCTCTGACCGGGCTGGCTGCCCGCCTGCTGGCCGATAAAGCGATGGAAGAGGCCGCCCTGCACAACGC
TGAAGCGGCTGCCGCCAAGGCAAACAAGCCCCTGGCCCGCTATCTGCTGGAAAAAAAACTCGTTGACGGCAAAGCACTGA
TTCTGGCGATTGCCGAAGAGTTTGGCCTGCCAGTGTTCGACCTCAGTGCCTTTGATAAAGAGCACCTTGTTAAAGAGGTT
GTAGAGGAAAAGCTGATCCGAAAACACAATGTGCTGCCGCTGATCAAACGGGGCTCGCACCTGTTTGTCGCGGTTTCCGA
CCCTTCCAACCTGCAGAGCCTGGATGAAATCCGCTTTAATACCGGCCTGACCATTCATACCGTACTGTCCGAGCCCGACC
GCCTGGAGAAAATGATTGATGCCGCCCTGGATGATACCAGCGGCGCGTTTTCAGATCTTGGCGACGATGGCCTGGTTGAT
CTTGAAGACCTTGAAGTCAGTGCCATTGATGACGGGGCAAATGACGCTCAGGGCACTATCGGTGGTGGAGAAGATGATAC
TCCGGTGGTTAAGTTCGTTAATAAAATGCTGCTTTCCGCCATCAAAGGGGGTGCATCGGATCTTCACTTTGAGCCTTATG
AAAGAGCCTACCGGGTGCGTTTCAGAACCGACGGTGTGCTCCATGAAGTCAGTCGCCCTCCGGTCACTCTTGGCTCCCGG
ATTGCCTCCCGTCTGAAGATCATGTCGGCCATGGACATTTCCGAGCGCAGAAAGCCTCAGGATGGCCGGATAAAAATGAA
AATTTCCAAAACCAAGGCCATCGACTTCCGTGTCAACACCCTGCCGACGCTCTGGGGGGAGAAAATCGTCCTTCGTATCC
TCGATCCTTCCAGTGCCAAAATGGGGATTGATGCCCTGGGCTATGAAGCCGAGCAGAAGGAGATGTACCTGAACGCCCTG
CACCAGCCACAGGGTATGATTCTGGTCACCGGCCCTACCGGTTCGGGCAAAACCGTCTCTCTGTATACCGGTTTGAATAT
TCTGAATACCAATGAAAGGAACATCTCCACGGCGGAAGATCCGGTGGAAATCAACCTGGAAGGGATTAACCAGGTCAACG
TCAACCCCAGACAGGGCATGGACTTCAGCCAAGCCTTGAGAGCGTTTCTACGTCAGGACCCCGATATCATCATGGTGGGT
GAGATACGGGATCTGGAAACGGCCAATATTGCCATCAAGGCAGCCCAGACCGGTCACATGGTGATGTCCACACTGCACAC
CAACAGTGCGGCAGAGACCCTGACCCGTTTGCAGAATATGGGGGTTCCCTCATTTAACATTGCCACCTCTGTCAGTCTGA
TTATTGCCCAGCGCCTGGCGCGAAGGCTGTGCAATCACTGCAAGGTGAAGGTGCAAATCCCCAAAGAAACACTGCTGGAA
CAGGGCTTTACTGAAGAGCAGGCGGACTCCGCCGAGATTTTTGGTCCTAAAGGCTGTGAAAACTGCAAGAGCGGTTATAA
AGGTCGTGTGGGTATTTATGAGGTGGTGTCGATCACCCCCGGTATGCAGCAGATTATTATGGCCGAAGGTAACTCTCTGG
AAATCTCGAAACAGGCACAGAAGGAAGGCTTTAATAACCTGCGCCAATCCGGTCTGTTAAAGGCTTTGCAAGGTGTGACC
AGTCTCGAAGAGGTCAACCGGGTAACGCAGGATTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.93

99.65

0.597
  pilB Acinetobacter baylyi ADP1

57.193

99.825

0.571
  pilB Legionella pneumophila strain ERS1305867

55.185

99.65

0.55
  pilB Vibrio cholerae strain A1552

51.311

93.52

0.48
  pilF Neisseria gonorrhoeae MS11

48.833

97.548

0.476
  pilB Vibrio campbellii strain DS40M4

48.571

98.074

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

51.562

89.667

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

39.884

90.893

0.363