Detailed information NTG0656556

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: MIH19_RS03065
• Genome accession: NZ_CP092289
• Coordinates: 644809..645930 (+)
• Length: 373 a.a.
• NCBI ID: WP_249007510.1
• Uniprot ID: -
• Organism: Marinobacter sp. M1C
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 639809..650930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MIH19_RS03045 (MIH19_03045)	-	641346..641930 (-)	585	WP_249007506.1	YggT family protein	-
MIH19_RS03050 (MIH19_03050)	proC	641956..642786 (-)	831	WP_249007507.1	pyrroline-5-carboxylate reductase	-
MIH19_RS03055 (MIH19_03055)	-	642860..643564 (-)	705	WP_249007508.1	YggS family pyridoxal phosphate-dependent enzyme	-
MIH19_RS03060 (MIH19_03060)	pilT	643701..644735 (+)	1035	WP_249007509.1	type IV pilus twitching motility protein PilT	Machinery gene
MIH19_RS03065 (MIH19_03065)	pilU	644809..645930 (+)	1122	WP_249007510.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
MIH19_RS03070 (MIH19_03070)	-	646048..647478 (+)	1431	WP_249007511.1	outer membrane protein transport protein	-
MIH19_RS03075 (MIH19_03075)	polA	647575..650370 (-)	2796	WP_249007512.1	DNA polymerase I	-
MIH19_RS03080 (MIH19_03080)	-	650363..650536 (-)	174	WP_249007513.1	hypothetical protein	-
MIH19_RS03085 (MIH19_03085)	-	650566..650901 (+)	336	WP_249007514.1	DUF2782 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41438.74 Da
• Isoelectric Point: 6.6614

>NTDB_id=656556 MIH19_RS03065 WP_249007510.1 644809..645930(+) (pilU) [Marinobacter sp. M1C]
MEFEKLLRLMVEKGGSDLFITAGVAPSMKVNGKVWPVTKTALTPDMTREMVYGAMSDKQRAEFDESHECNFAISARGIGR
FRVSAFFQRNQCGMVLRRIEVKIPEIDDLSLPDIVKELAMTKRGLIMFVGATGTGKSTSLAAMIGFRNHNSRGHIISIED
PIEFIHQHQGCIVTQREVGIDTASFEVALKNTLRQAPDVILIGEVRTRQTMEYSVQFAETGHLCLATLHANNANQALDRI
IQFFPPEQHGQIWMDLSLNLRAIVAQQLVPTPDGKGRKAVIEVLINTPLAADLIRKGEVHKLKDLMAKSNEIGMRTFDQA
LYELYSEGSVTYEDALAHADSPNDLRLMIKLGTDAKGVDKLSSSVDKLTIQQD

Nucleotide

• Length: 1122 bp

>NTDB_id=656556 MIH19_RS03065 WP_249007510.1 644809..645930(+) (pilU) [Marinobacter sp. M1C]
ATGGAATTTGAAAAGCTACTGCGGCTGATGGTTGAGAAAGGCGGCTCGGACTTGTTCATTACCGCCGGCGTTGCGCCCAG
TATGAAAGTGAACGGTAAAGTGTGGCCTGTCACAAAGACCGCCCTAACACCCGATATGACCCGTGAGATGGTTTACGGTG
CCATGAGCGACAAGCAACGCGCCGAGTTTGATGAAAGCCACGAGTGTAACTTCGCCATCAGCGCCCGTGGCATTGGTCGC
TTTCGGGTTAGCGCGTTTTTTCAGCGCAATCAGTGCGGTATGGTGCTGCGTCGTATTGAAGTAAAGATACCGGAAATCGA
CGATCTTTCACTGCCCGACATCGTTAAAGAGCTGGCTATGACCAAGCGCGGGCTGATCATGTTTGTCGGCGCCACCGGCA
CGGGCAAATCCACCTCGTTGGCTGCGATGATTGGCTTCCGCAACCACAACAGCCGCGGCCATATCATTTCTATTGAAGAC
CCGATTGAGTTTATACACCAACATCAGGGTTGCATTGTGACCCAGCGCGAAGTGGGCATAGATACTGCCAGCTTTGAAGT
GGCGTTGAAAAACACGCTGCGCCAGGCGCCAGATGTGATTTTGATTGGTGAGGTGCGAACCCGCCAGACCATGGAATATT
CAGTACAGTTCGCCGAAACCGGGCACTTGTGCCTGGCGACTCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
ATCCAGTTTTTTCCACCCGAACAGCACGGCCAAATCTGGATGGACCTGTCGCTGAACCTTCGCGCTATTGTTGCGCAGCA
ACTGGTGCCCACGCCAGACGGCAAGGGCCGGAAAGCGGTTATTGAAGTGCTGATCAATACGCCGCTGGCGGCTGACCTGA
TTCGCAAAGGCGAAGTGCACAAGCTCAAAGACCTGATGGCCAAATCCAACGAAATTGGTATGCGCACTTTTGATCAGGCG
CTGTATGAGCTGTATTCCGAAGGTTCCGTGACTTATGAAGATGCCTTGGCACATGCTGACTCACCCAACGATTTGCGTTT
GATGATAAAGCTTGGAACCGACGCCAAAGGGGTGGACAAGTTGTCGTCTTCGGTGGATAAGCTGACCATTCAGCAGGACT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	78.706	99.464	0.783
pilU	Acinetobacter baylyi ADP1	65.449	95.442	0.625
pilU	Vibrio cholerae strain A1552	54.324	99.196	0.539
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.563	95.174	0.386