Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   MBG98_RS04855 Genome accession   NZ_CP092038
Coordinates   1052798..1053397 (+) Length   199 a.a.
NCBI ID   WP_011213355.1    Uniprot ID   -
Organism   Legionella pneumophila strain 11052018-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047798..1058397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MBG98_RS04840 (MBG98_04840) ravI 1049744..1050751 (-) 1008 WP_011946020.1 Dot/Icm T4SS effector RavI -
  MBG98_RS04845 (MBG98_04845) pilM 1051168..1052232 (+) 1065 WP_011946022.1 type IV pilus assembly protein PilM Machinery gene
  MBG98_RS04850 (MBG98_04850) pilN 1052236..1052784 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  MBG98_RS04855 (MBG98_04855) pilO 1052798..1053397 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  MBG98_RS04860 (MBG98_04860) pilP 1053394..1053978 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  MBG98_RS04865 (MBG98_04865) pilQ 1053982..1056078 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  MBG98_RS04870 (MBG98_04870) aroK 1056525..1057052 (+) 528 WP_010946667.1 shikimate kinase AroK -
  MBG98_RS04875 (MBG98_04875) aroB 1057039..1058148 (+) 1110 WP_027264601.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22523.24 Da        Isoelectric Point: 5.3369

>NTDB_id=655285 MBG98_RS04855 WP_011213355.1 1052798..1053397(+) (pilO) [Legionella pneumophila strain 11052018-5]
MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=655285 MBG98_RS04855 WP_011213355.1 1052798..1053397(+) (pilO) [Legionella pneumophila strain 11052018-5]
ATGGACACAGTCAATTTAAATGAATTAACTCTGGAGAATGTCGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTAATTTT
AGGCATCAGTATACTGATAATTGGATTAGGATATTGGCTTATCGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAGACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCTTATCGTAATCAGTTA
CAGCTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTCCCGGCGAAAAATGAAATGCCTGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATCGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACCTTGCATGAATTTAGTGTTGAGGGGGTATCATCCGGGGATGGTAAAACAGTCTCTCAGGATGAATTGGTGAT
GGCTATTACTGCGAAAATATACAGATACCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

100

100

1
  comO Acinetobacter baylyi ADP1

39.791

95.98

0.382
  pilO Acinetobacter baumannii D1279779

38.22

95.98

0.367