Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MBG98_RS04845 Genome accession   NZ_CP092038
Coordinates   1051168..1052232 (+) Length   354 a.a.
NCBI ID   WP_011946022.1    Uniprot ID   -
Organism   Legionella pneumophila strain 11052018-5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1046168..1057232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MBG98_RS04835 (MBG98_04835) - 1047208..1049592 (-) 2385 WP_011946019.1 penicillin-binding protein 1A -
  MBG98_RS04840 (MBG98_04840) ravI 1049744..1050751 (-) 1008 WP_011946020.1 Dot/Icm T4SS effector RavI -
  MBG98_RS04845 (MBG98_04845) pilM 1051168..1052232 (+) 1065 WP_011946022.1 type IV pilus assembly protein PilM Machinery gene
  MBG98_RS04850 (MBG98_04850) pilN 1052236..1052784 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  MBG98_RS04855 (MBG98_04855) pilO 1052798..1053397 (+) 600 WP_011213355.1 type 4a pilus biogenesis protein PilO Machinery gene
  MBG98_RS04860 (MBG98_04860) pilP 1053394..1053978 (+) 585 WP_011213356.1 pilus assembly protein PilP Machinery gene
  MBG98_RS04865 (MBG98_04865) pilQ 1053982..1056078 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  MBG98_RS04870 (MBG98_04870) aroK 1056525..1057052 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38818.82 Da        Isoelectric Point: 5.0367

>NTDB_id=655283 MBG98_RS04845 WP_011946022.1 1051168..1052232(+) (pilM) [Legionella pneumophila strain 11052018-5]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPTSALEGNMIKDVDVVANCIKKLVERLRFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=655283 MBG98_RS04845 WP_011946022.1 1051168..1052232(+) (pilM) [Legionella pneumophila strain 11052018-5]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCTGTGAAAATTTTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGGGAAGAAATACCAACCTCTGCTCTTGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAGGTTTACATCTAAAAATGTGGCATTG
GCTGTGCCTGATTCAGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGATTGACTGATCTTGAAATGGAGGAGCTTGT
CGTTATTGAGGCGGATAAGTATATCCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCGGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

99.435

100

0.994
  comM Acinetobacter nosocomialis M2

42.373

100

0.424
  pilM Acinetobacter baumannii D1279779

42.09

100

0.421
  comM Acinetobacter baylyi ADP1

41.176

100

0.415