NTDB

Overview

Name	comM	Type	Machinery gene
Locus tag	L8E65_RS28535	Genome accession	NZ_CP091880
Coordinates	6146942..6148435 (+)	Length	497 a.a.
NCBI ID	WP_003098248.1	Uniprot ID	Q9HTR4
Organism	Pseudomonas aeruginosa strain US449
Function	require for natural transformation (predicted from homology) Unclear

Genomic Context

Location: 6141942..6153435

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L8E65_RS28505 (L8E65_28490)	-	6142064..6142978 (-)	915	WP_003118059.1	fimbrial protein	-
L8E65_RS28510 (L8E65_28495)	sutA	6143414..6143731 (-)	318	WP_003098240.1	transcriptional regulator SutA	-
L8E65_RS28515 (L8E65_28500)	-	6143809..6144234 (-)	426	WP_003096441.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
L8E65_RS28520 (L8E65_28505)	-	6144495..6145823 (-)	1329	WP_003109815.1	ammonium transporter	-
L8E65_RS28525 (L8E65_28510)	glnK	6145863..6146201 (-)	339	WP_003096476.1	P-II family nitrogen regulator	-
L8E65_RS28530 (L8E65_28515)	-	6146641..6146901 (+)	261	WP_003096478.1	accessory factor UbiK family protein	-
L8E65_RS28535 (L8E65_28520)	comM	6146942..6148435 (+)	1494	WP_003098248.1	YifB family Mg chelatase-like AAA ATPase	Machinery gene
L8E65_RS28540 (L8E65_28525)	betT	6148560..6150545 (+)	1986	WP_047689573.1	choline BCCT transporter BetT	-
L8E65_RS28545 (L8E65_28530)	pchP	6150588..6151637 (-)	1050	WP_003110458.1	phosphorylcholine phosphatase	-
L8E65_RS28550 (L8E65_28535)	-	6151788..6152705 (-)	918	WP_047689576.1	LysR substrate-binding domain-containing protein	-

Sequence

Protein

Download Length: 497 a.a. Molecular weight: 53083.04 Da Isoelectric Point: 7.9587

                                >NTDB_id=654207 L8E65_RS28535 WP_003098248.1 6146942..6148435(+) (comM) [Pseudomonas aeruginosa strain US449]

MSLAIVHSRAQVGVEAPCVSVEAHLANGLPSLTLVGLPETAVRESKDRVRSALLNAGFDFPARRITLNLAPADLPKDGGR

FDLAIALGILAASGQLPGTALDGLECLGELALSGAIRPVRGVLPAALAARDARRVLVVPKENAEEASLASGLTVFAVDHL

LEIAGHLSGQAPLPPYQARGLLRAPFPYPDLAEVQGQAAAKRALLVAAAGAHNLLLSGPPGTGKTLLASRLPGLLPALDE

DEALEVAAIHSVASHVPLRHWPQRPFRQPHHSASAPALVGGGSRPQPGEITLAHQGVLFLDELPEFERKVLEVLREPLES

GEIVIARANGRVRFPARFQLVAAMNPCPCGYLGDPSGRCRCTPEQVQRYRGKLSGPLLDRIDLHVSVLRESTSLQPGHGE

TATAEVSERVGAARQRQLARQGCANAHLDLQAMHRNCALAEADRRWLEAAGERLELSLRALHRILKVARTLADLERIDAI

ERRHLAEALQYRAMTST

Nucleotide

Download Length: 1494 bp

                                >NTDB_id=654207 L8E65_RS28535 WP_003098248.1 6146942..6148435(+) (comM) [Pseudomonas aeruginosa strain US449]

ATGTCCCTGGCGATTGTCCACAGCCGAGCCCAGGTCGGCGTCGAAGCCCCCTGCGTCAGCGTCGAGGCGCACCTGGCCAA

CGGCCTGCCTTCGCTGACCCTGGTCGGCCTGCCGGAAACCGCGGTGCGCGAGAGCAAGGACCGCGTGCGCAGCGCCCTGC

TCAATGCCGGTTTCGACTTCCCCGCGCGGCGCATCACCCTCAACCTCGCCCCCGCCGACCTGCCCAAGGACGGCGGTCGC

TTCGACCTGGCCATCGCACTCGGCATCCTCGCCGCCAGCGGCCAGTTGCCCGGCACCGCCCTCGACGGCCTGGAGTGCCT

TGGCGAACTGGCCCTGTCCGGGGCGATCCGGCCAGTGCGAGGCGTATTGCCGGCCGCGCTGGCGGCGCGCGACGCAAGGC

GCGTTCTGGTGGTACCGAAGGAAAATGCCGAAGAGGCCAGCCTGGCCAGCGGGCTGACGGTGTTCGCCGTGGACCACCTG

CTGGAGATCGCCGGACACCTCTCCGGCCAGGCCCCGCTGCCGCCCTACCAGGCCCGCGGCCTGCTCCGCGCGCCCTTCCC

TTATCCAGACCTGGCCGAGGTCCAGGGCCAGGCCGCCGCCAAGCGCGCCCTGCTGGTGGCCGCCGCCGGCGCGCACAACC

TGTTGCTCAGCGGCCCGCCGGGCACCGGCAAGACCCTCCTGGCCAGCCGCCTGCCCGGCCTGCTGCCGGCGCTCGACGAG

GACGAAGCCCTGGAGGTCGCGGCGATCCATTCGGTGGCCAGCCACGTCCCCCTCAGGCACTGGCCGCAGCGACCGTTCCG

CCAGCCGCACCACTCCGCCTCCGCGCCGGCCCTGGTCGGCGGCGGCAGCCGCCCGCAGCCGGGCGAGATCACCCTGGCGC

ACCAGGGCGTGCTGTTCCTCGACGAACTGCCGGAGTTCGAGCGCAAGGTCCTGGAGGTCCTGCGCGAGCCGCTGGAAAGC

GGCGAGATCGTCATTGCCCGGGCCAACGGCCGGGTACGTTTCCCGGCGCGCTTCCAACTGGTGGCGGCGATGAATCCCTG

TCCCTGTGGCTACCTCGGCGATCCCAGTGGCCGCTGCCGCTGCACCCCGGAACAGGTCCAGCGCTACCGGGGCAAGCTGT

CCGGACCGCTGCTCGATCGCATCGACCTGCACGTCAGCGTGCTCCGCGAAAGCACCAGCCTGCAGCCAGGACACGGCGAA

ACCGCTACCGCCGAGGTCAGCGAACGGGTGGGCGCCGCACGGCAACGGCAACTGGCCCGCCAGGGCTGCGCCAATGCCCA

TCTCGATCTCCAGGCGATGCACCGCAATTGTGCACTCGCCGAAGCGGACCGCCGCTGGCTGGAGGCTGCCGGAGAGCGCC

TGGAACTTTCCTTGCGCGCCTTGCATCGCATACTCAAGGTGGCCCGGACGCTGGCCGACCTGGAGCGCATCGATGCCATC

GAACGCCGGCACCTGGCGGAAGCCCTGCAGTATCGGGCAATGACCTCCACGTGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(190-390)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(402-492)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q9HTR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comM	Haemophilus influenzae Rd KW20	55.186	100	0.567
comM	Vibrio campbellii strain DS40M4	56.74	100	0.567
comM	Vibrio cholerae strain A1552	56.338	100	0.563
comM	Glaesserella parasuis strain SC1401	55.179	100	0.557
comM	Legionella pneumophila str. Paris	50.1	100	0.503
comM	Legionella pneumophila strain ERS1305867	50.1	100	0.503
RA0C_RS07335	Riemerella anatipestifer ATCC 11845 = DSM 15868	45.866	100	0.469

Detailed information NTG0654207