Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   LVJ84_RS00105 Genome accession   NZ_CP091516
Coordinates   16777..17325 (+) Length   182 a.a.
NCBI ID   WP_172461201.1    Uniprot ID   A0A377R156
Organism   Kingella potus strain 3/SID/1128     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 11777..22325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ84_RS00080 (LVJ84_00080) asd 12011..12913 (-) 903 WP_115307791.1 archaetidylserine decarboxylase -
  LVJ84_RS00085 (LVJ84_00085) ubiM 13134..14318 (+) 1185 WP_244731943.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  LVJ84_RS00090 (LVJ84_00090) - 14495..14737 (+) 243 WP_244731946.1 hypothetical protein -
  LVJ84_RS00095 (LVJ84_00095) - 14734..15696 (+) 963 WP_244731949.1 alpha/beta hydrolase-fold protein -
  LVJ84_RS00100 (LVJ84_00100) mscL 15863..16303 (+) 441 WP_115307794.1 large conductance mechanosensitive channel protein MscL -
  LVJ84_RS00105 (LVJ84_00105) pilE 16777..17325 (+) 549 WP_172461201.1 pilin Machinery gene
  LVJ84_RS00115 (LVJ84_00115) - 17408..17962 (+) 555 WP_280529836.1 pilin -
  LVJ84_RS00120 (LVJ84_00120) tfpZ 18014..18799 (+) 786 WP_115307795.1 TfpX/TfpZ family type IV pilin accessory protein -
  LVJ84_RS00125 (LVJ84_00125) pilE 19257..19805 (+) 549 WP_172461201.1 pilin Machinery gene
  LVJ84_RS00135 (LVJ84_00135) - 19888..20442 (+) 555 WP_280529836.1 pilin -
  LVJ84_RS00140 (LVJ84_00140) tfpZ 20494..21279 (+) 786 WP_115307795.1 TfpX/TfpZ family type IV pilin accessory protein -
  LVJ84_RS00145 (LVJ84_00145) pilE 21737..22285 (+) 549 WP_172461201.1 pilin Machinery gene

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 19183.97 Da        Isoelectric Point: 6.3357

>NTDB_id=651946 LVJ84_RS00105 WP_172461201.1 16777..17325(+) (pilE) [Kingella potus strain 3/SID/1128]
MKAMQKGFTLIELMIVVAIIGILAAIALPAYQDYTVRSRITEGLNLAESAKTMLATDATSSNDLDIAIAAWNRQVNNKGA
TSKYVTSVLMEAASGDEKRGELTVTFDGAAVGVKSDQNTLVLTPWINDGTNKIRLAKALVDGVAGNLDWSCQSATTATAV
KQIGTAGTVGTVLEKYAPSQCR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=651946 LVJ84_RS00105 WP_172461201.1 16777..17325(+) (pilE) [Kingella potus strain 3/SID/1128]
ATGAAAGCAATGCAAAAAGGTTTCACCCTGATCGAGTTGATGATCGTTGTCGCCATTATCGGCATCTTGGCCGCTATCGC
CCTGCCGGCCTACCAAGACTACACAGTACGCAGCCGCATTACCGAGGGTTTGAACTTGGCTGAGTCTGCTAAAACCATGC
TCGCTACCGATGCTACTTCCAGCAATGACTTGGACATCGCTATAGCCGCTTGGAATCGTCAGGTTAACAACAAAGGTGCT
ACTTCCAAATATGTAACCAGCGTTTTGATGGAAGCTGCTTCCGGCGACGAAAAACGCGGCGAGCTGACTGTTACATTCGA
CGGTGCTGCCGTTGGTGTGAAATCGGATCAAAATACTTTGGTTTTGACTCCTTGGATCAATGACGGTACTAACAAAATCC
GTTTGGCTAAGGCGTTGGTTGACGGCGTTGCCGGTAACCTCGACTGGTCCTGCCAATCCGCTACTACTGCTACCGCTGTG
AAACAAATCGGTACTGCTGGTACTGTTGGCACCGTGCTGGAAAAATACGCTCCTTCGCAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-182)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A377R156

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

43.781

100

0.484
  pilA Ralstonia pseudosolanacearum GMI1000

41.209

100

0.412
  pilA2 Legionella pneumophila str. Paris

41.011

97.802

0.401
  pilA2 Legionella pneumophila strain ERS1305867

40.449

97.802

0.396
  pilE Neisseria gonorrhoeae strain FA1090

35.106

100

0.363