Detailed information NTG0650614

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L2Z07_RS18680
• Genome accession: NZ_CP091367
• Coordinates: 3888799..3889917 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain AB169-VUB
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3883799..3894917

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z07_RS18650 (L2Z07_18645)	-	3884628..3885476 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
L2Z07_RS18655 (L2Z07_18650)	-	3885729..3886184 (+)	456	WP_000782976.1	bacteriohemerythrin	-
L2Z07_RS18660 (L2Z07_18655)	-	3886337..3887404 (+)	1068	WP_000092403.1	hypothetical protein	-
L2Z07_RS18665 (L2Z07_18660)	-	3887404..3887727 (+)	324	WP_000442585.1	RnfH family protein	-
L2Z07_RS18670 (L2Z07_18665)	bamE	3887782..3888180 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
L2Z07_RS18675 (L2Z07_18670)	fur	3888292..3888729 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
L2Z07_RS18680 (L2Z07_18675)	pilU	3888799..3889917 (-)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L2Z07_RS18685 (L2Z07_18680)	pilT	3889945..3890982 (-)	1038	WP_225488298.1	type IV pilus twitching motility protein PilT	Machinery gene
L2Z07_RS18690 (L2Z07_18685)	-	3891109..3891801 (+)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=650614 L2Z07_RS18680 WP_000347036.1 3888799..3889917(-) (pilU) [Acinetobacter baumannii strain AB169-VUB]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=650614 L2Z07_RS18680 WP_000347036.1 3888799..3889917(-) (pilU) [Acinetobacter baumannii strain AB169-VUB]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392