Detailed information NTG0649752

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: L2Z32_RS14205
• Genome accession: NZ_CP091344
• Coordinates: 2985471..2986529 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain AB220-VUB
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2980471..2991529

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Z32_RS14190 (L2Z32_14185)	-	2981576..2981908 (+)	333	WP_000169594.1	hypothetical protein	-
L2Z32_RS14195 (L2Z32_14190)	-	2981911..2982735 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
L2Z32_RS14200 (L2Z32_14195)	ponA	2982753..2985308 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
L2Z32_RS14205 (L2Z32_14200)	pilM	2985471..2986529 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
L2Z32_RS14210 (L2Z32_14205)	pilN	2986529..2987170 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
L2Z32_RS14215 (L2Z32_14210)	pilO	2987167..2987907 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
L2Z32_RS14220 (L2Z32_14215)	pilP	2987918..2988445 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
L2Z32_RS14225 (L2Z32_14220)	pilQ	2988513..2990678 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
L2Z32_RS14230 (L2Z32_14225)	aroK	2990690..2991259 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=649752 L2Z32_RS14205 WP_002014066.1 2985471..2986529(+) (pilM) [Acinetobacter baumannii strain AB220-VUB]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=649752 L2Z32_RS14205 WP_002014066.1 2985471..2986529(+) (pilM) [Acinetobacter baumannii strain AB220-VUB]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423