Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   L2Z32_RS14215 Genome accession   NZ_CP091344
Coordinates   2987167..2987907 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain AB220-VUB     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982167..2992907
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L2Z32_RS14200 (L2Z32_14195) ponA 2982753..2985308 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  L2Z32_RS14205 (L2Z32_14200) pilM 2985471..2986529 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  L2Z32_RS14210 (L2Z32_14205) pilN 2986529..2987170 (+) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  L2Z32_RS14215 (L2Z32_14210) pilO 2987167..2987907 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  L2Z32_RS14220 (L2Z32_14215) pilP 2987918..2988445 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  L2Z32_RS14225 (L2Z32_14220) pilQ 2988513..2990678 (+) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  L2Z32_RS14230 (L2Z32_14225) aroK 2990690..2991259 (+) 570 WP_000643712.1 shikimate kinase AroK -
  L2Z32_RS14235 (L2Z32_14230) aroB 2991288..2992370 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=649754 L2Z32_RS14215 WP_000076101.1 2987167..2987907(+) (pilO) [Acinetobacter baumannii strain AB220-VUB]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=649754 L2Z32_RS14215 WP_000076101.1 2987167..2987907(+) (pilO) [Acinetobacter baumannii strain AB220-VUB]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGCATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683