Detailed information NTG0648043

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L2Y01_RS12880
• Genome accession: NZ_CP091147
• Coordinates: 2784570..2785739 (+)
• Length: 389 a.a.
• NCBI ID: WP_017682837.1
• Uniprot ID: A0A0K8M896
• Organism: Pseudomonas avellanae strain NCPPB 4222
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2779570..2790739

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Y01_RS12865 (L2Y01_12865)	-	2779933..2782266 (+)	2334	WP_024419638.1	ATP-dependent DNA helicase	-
L2Y01_RS12870 (L2Y01_12870)	pgm	2782367..2784013 (+)	1647	WP_005618490.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
L2Y01_RS12875 (L2Y01_12875)	-	2784140..2784415 (-)	276	WP_003373489.1	peptidylprolyl isomerase	-
L2Y01_RS12880 (L2Y01_12880)	pilU	2784570..2785739 (+)	1170	WP_017682837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L2Y01_RS12885 (L2Y01_12885)	-	2786102..2787679 (-)	1578	WP_005618489.1	ABC transporter ATP-binding protein	-
L2Y01_RS12890 (L2Y01_12890)	-	2787681..2788703 (-)	1023	WP_005618488.1	ABC transporter permease	-
L2Y01_RS12895 (L2Y01_12895)	-	2788703..2789767 (-)	1065	WP_011104338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42669.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=648043 L2Y01_RS12880 WP_017682837.1 2784570..2785739(+) (pilU) [Pseudomonas avellanae strain NCPPB 4222]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=648043 L2Y01_RS12880 WP_017682837.1 2784570..2785739(+) (pilU) [Pseudomonas avellanae strain NCPPB 4222]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAATTCAACGGCGTGCTCAAACCGCTGGGCAGCGAAACGCTCAAGCCCGGCGAAGTGGCGGTGGTTGCTCAAGGGC
TTATGGACGAGGAACAGAAGCTCGAGTTTCTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCTGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTGGACATTCCGCGCTTTGA
GGACCTGTTCCTGCCGCCGGTGTTGCTTGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGTAAATCTACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACCTTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGCGCGCAGCTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGCAACGAACTGACCGAGCTCAAGGGCATCATGGAAAAGTCTGGCAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGAAGGTGCGATTAGCGAGGAAGAGGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAGCTGCACAGCGAGAACGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A0K8M896

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365