Detailed information NTG0647992

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: L2Y00_RS04530
• Genome accession: NZ_CP091143
• Coordinates: 1005160..1006329 (+)
• Length: 389 a.a.
• NCBI ID: WP_017682837.1
• Uniprot ID: A0A0K8M896
• Organism: Pseudomonas avellanae strain NCPPB 3491
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1000160..1011329

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L2Y00_RS04515 (L2Y00_04515)	-	1000529..1002856 (+)	2328	WP_005618491.1	ATP-dependent DNA helicase	-
L2Y00_RS04520 (L2Y00_04520)	pgm	1002957..1004603 (+)	1647	WP_005618490.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
L2Y00_RS04525 (L2Y00_04525)	-	1004730..1005005 (-)	276	WP_003373489.1	peptidylprolyl isomerase	-
L2Y00_RS04530 (L2Y00_04530)	pilU	1005160..1006329 (+)	1170	WP_017682837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
L2Y00_RS04535 (L2Y00_04535)	-	1006692..1008269 (-)	1578	WP_005618489.1	ABC transporter ATP-binding protein	-
L2Y00_RS04540 (L2Y00_04540)	-	1008271..1009293 (-)	1023	WP_005618488.1	ABC transporter permease	-
L2Y00_RS04545 (L2Y00_04545)	-	1009293..1010357 (-)	1065	WP_005618487.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42669.81 Da
• Isoelectric Point: 5.8676

>NTDB_id=647992 L2Y00_RS04530 WP_017682837.1 1005160..1006329(+) (pilU) [Pseudomonas avellanae strain NCPPB 3491]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVVAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSENGVTSLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=647992 L2Y00_RS04530 WP_017682837.1 1005160..1006329(+) (pilU) [Pseudomonas avellanae strain NCPPB 3491]
ATGGATTTCCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAATTCAACGGCGTGCTCAAACCGCTGGGCAGCGAAACGCTCAAGCCCGGCGAAGTGGCGGTGGTTGCTCAAGGGC
TTATGGACGAGGAACAGAAGCTCGAGTTTCTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCTGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCCATTGTCGCGCGTAACATCAAGCTGGACATTCCGCGCTTTGA
GGACCTGTTCCTGCCGCCGGTGTTGCTTGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGTAAATCTACCTCGCTGGCGGCACTGATCGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGGGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACCTTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATTCGCGATCGCGAAACCATGGAGCACG
CGCTGGCATTCGCCGACACCGGCCACCTGGCGATTTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGAATC
ATCAACTTCTTCCCGGAAGAGCGGCGCGCGCAGCTGTTGCACGACCTTGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAGGTGATGATGGGCACGCCGACCATCCGCGATCTGATCC
AGCGCAACGAACTGACCGAGCTCAAGGGCATCATGGAAAAGTCTGGCAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGAAGGTGCGATTAGCGAGGAAGAGGCGTTGAAGAATGCCGACTCGCAAAACAACGTGCGCCTGCG
CCTCAAGCTGCACAGCGAGAACGGAGTGACCAGCCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A0K8M896

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	51.831	91.26	0.473
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.426	98.458	0.388
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365