Detailed information NTG0645860

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: L1A16_RS09575
• Genome accession: NZ_CP090885
• Coordinates: 1858963..1859424 (-)
• Length: 153 a.a.
• NCBI ID: WP_000250542.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain ND6401
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1853963..1864424

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L1A16_RS09545 (L1A16_09545)	-	1854267..1855091 (-)	825	WP_001809383.1	membrane protein insertase YidC	-
L1A16_RS09550 (L1A16_09550)	rnpA	1855066..1855437 (-)	372	WP_000739244.1	ribonuclease P protein component	-
L1A16_RS10740	-	1855454..1855585 (-)	132	WP_000768904.1	hypothetical protein	-
L1A16_RS09555 (L1A16_09555)	-	1855586..1856776 (-)	1191	WP_000167765.1	acetate kinase	-
L1A16_RS09560 (L1A16_09560)	comYH	1856827..1857780 (-)	954	WP_000345099.1	class I SAM-dependent methyltransferase	Machinery gene
L1A16_RS09565 (L1A16_09565)	-	1857841..1858435 (-)	595	Protein_1878	methyltransferase domain-containing protein	-
L1A16_RS09570 (L1A16_09570)	comGG/cglG	1858572..1858985 (-)	414	WP_000265624.1	competence type IV pilus minor pilin ComGG	Machinery gene
L1A16_RS09575 (L1A16_09575)	comGF/cglF	1858963..1859424 (-)	462	WP_000250542.1	competence type IV pilus minor pilin ComGF	Machinery gene
L1A16_RS09580 (L1A16_09580)	comGE/cglE	1859387..1859689 (-)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
L1A16_RS09585 (L1A16_09585)	comGD/cglD	1859652..1860056 (-)	405	WP_000588013.1	competence type IV pilus minor pilin ComGD	Machinery gene
L1A16_RS09590 (L1A16_09590)	comGC/cglC	1860049..1860375 (-)	327	WP_000738636.1	comG operon protein ComGC	Machinery gene
L1A16_RS09595 (L1A16_09595)	comGB/cglB	1860377..1861393 (-)	1017	WP_238881034.1	competence type IV pilus assembly protein ComGB	Machinery gene
L1A16_RS09600 (L1A16_09600)	comGA/cglA/cilD	1861341..1862282 (-)	942	WP_000249552.1	competence type IV pilus ATPase ComGA	Machinery gene
L1A16_RS09605 (L1A16_09605)	-	1862358..1862723 (-)	366	WP_000286415.1	DUF1033 family protein	-
L1A16_RS09610 (L1A16_09610)	-	1862874..1863932 (-)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-

Sequence

Protein

• Length: 153 a.a.
• Molecular weight: 17918.53 Da
• Isoelectric Point: 9.3137

>NTDB_id=645860 L1A16_RS09575 WP_000250542.1 1858963..1859424(-) (comGF/cglF) [Streptococcus pneumoniae strain ND6401]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEKS

Nucleotide

• Length: 462 bp

>NTDB_id=645860 L1A16_RS09575 WP_000250542.1 1858963..1859424(-) (comGF/cglF) [Streptococcus pneumoniae strain ND6401]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTACTTGCCCTCAT
TGTCATCAGTGGGGGATTACTCCTTTTTCAAACTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACGAATGCTCG
TGGTCGAGGTTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAAAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (45-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	96.078	100	0.961
comGF/cglF	Streptococcus pneumoniae D39	96.078	100	0.961
comGF/cglF	Streptococcus pneumoniae R6	96.078	100	0.961
comGF/cglF	Streptococcus pneumoniae TIGR4	96.078	100	0.961
comGF/cglF	Streptococcus mitis SK321	92.157	100	0.922
comGF/cglF	Streptococcus mitis NCTC 12261	90.85	100	0.909