Detailed information NTG0064509

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: SMSK321_RS08430
• Genome accession: NZ_AEDT01000026
• Coordinates: 15195..16262 (+)
• Length: 355 a.a.
• NCBI ID: WP_000159549.1
• Uniprot ID: -
• Organism: Streptococcus mitis SK321
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 10195..21262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMSK321_RS08415 (SMSK321_1106)	amiA	10718..12697 (+)	1980	WP_000749707.1	peptide ABC transporter substrate-binding protein	Regulator
SMSK321_RS08420 (SMSK321_1107)	amiC	12764..14260 (+)	1497	WP_000759912.1	ABC transporter permease	Regulator
SMSK321_RS08425 (SMSK321_1108)	amiD	14260..15186 (+)	927	WP_000103691.1	oligopeptide ABC transporter permease OppC	Regulator
SMSK321_RS08430 (SMSK321_1109)	amiE	15195..16262 (+)	1068	WP_000159549.1	ABC transporter ATP-binding protein	Regulator
SMSK321_RS08435 (SMSK321_1110)	amiF	16273..17199 (+)	927	WP_001291303.1	ATP-binding cassette domain-containing protein	Regulator
SMSK321_RS08440 (SMSK321_1111)	-	17651..18529 (-)	879	WP_001049297.1	LysR family transcriptional regulator	-
SMSK321_RS0102495 (SMSK321_1112)	-	18657..19007 (-)	351	WP_000208712.1	helix-turn-helix domain-containing protein	-
SMSK321_RS08445 (SMSK321_1113)	-	19366..20991 (+)	1626	WP_000123927.1	malolactic enzyme	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 39477.04 Da
• Isoelectric Point: 4.8041

>NTDB_id=64509 SMSK321_RS08430 WP_000159549.1 15195..16262(+) (amiE) [Streptococcus mitis SK321]
MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALVGESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDL
TALSSHKDWEQIRGAKIATIFQDPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFDEYPFQY
SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITHDLGVVASIADKVAVMYAGEIVE
YGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSIPGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAK
TWLLHEDAPKVEKPAVIANLHDKIRDKMGFAHLAD

Nucleotide

• Length: 1068 bp

>NTDB_id=64509 SMSK321_RS08430 WP_000159549.1 15195..16262(+) (amiE) [Streptococcus mitis SK321]
ATGACAAAAGAAAAAAATGTAATTTTGACTGCTCGCGATATTGTCGTGGAATTTGACGTTCGTGACAAAGTATTGACAGC
CATTCGAGGCGTTTCCCTTGAACTAGTTGAAGGAGAAGTATTAGCCTTGGTAGGTGAGTCAGGATCAGGAAAATCTGTTT
TGACAAAGACCTTCACTGGTATGCTCGAAGAAAATGGTCGAATTGCCCAAGGAAGCATTGACTACCGTGGTCAAGATTTG
ACAGCTTTATCTTCTCACAAGGATTGGGAACAAATTCGTGGTGCTAAGATTGCGACTATCTTCCAAGATCCAATGACTAG
TTTGGACCCAATTAAAACAATTGGTAGTCAGATTACAGAAGTTATTGTAAAACACCAAGGAAAAACAGCTAAAGAAGCGA
AAGAATTGGCCATTGACTACATGAATAAGGTTGGGATTCCAGACGCAGATAGACGTTTTGATGAATACCCATTCCAATAT
TCTGGAGGAATGCGTCAACGTATCGTTATTGCGATTGCCCTTGCCTGCCGACCTGATGTCTTGATCTGTGATGAGCCAAC
GACTGCCTTGGATGTGACTATCCAAGCACAGATTATTGATTTGCTAAAATCTTTACAAAACGAGTACCATTTCACAACAA
TCTTTATCACTCACGACCTTGGTGTGGTAGCAAGTATTGCGGATAAGGTAGCGGTTATGTATGCAGGAGAAATTGTTGAG
TATGGAACTGTTGAGGAAGTCTTCTATGACCCTCGCCATCCATATACATGGAGTCTCTTGTCTAGCTTGCCTCAGCTTGC
TGATGATAAAGGGGATCTTTACTCAATCCCAGGAACACCTCCGTCACTTTATACTGACCTGAAAGGGGATGCCTTTGCCT
TGCGTTCTGACTATGCAATGCAGATTGACTTCGAACAAAAAGCTCCTCAATTCTCAGTATCAGAGACACATTGGGCTAAA
ACTTGGCTACTTCATGAGGATGCTCCAAAAGTAGAAAAACCAGCTGTGATTGCAAATCTTCACGATAAGATCCGTGATAA
AATGGGATTTGCCCATCTGGCAGACTAG

Domains

Predicted by InterproScan.

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-301)

• ABC transporter (ABC_tran) (29-187)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	87.887	100	0.879
amiE	Streptococcus thermophilus LMG 18311	86.197	100	0.862
amiE	Streptococcus thermophilus LMD-9	86.197	100	0.862
oppD	Streptococcus mutans UA159	54.261	99.155	0.538