NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	OH217_RS17570	Genome accession	NZ_CP107281
Coordinates	3546466..3547590 (-)	Length	374 a.a.
NCBI ID	WP_000228551.1	Uniprot ID	P30852
Organism	Escherichia coli strain JCL301
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 3541466..3552590

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OH217_RS17540 (OH217_17415)	yrdA	3543266..3543820 (+)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-
OH217_RS17545 (OH217_17420)	yrdB	3543796..3544053 (-)	258	WP_001070563.1	DUF1488 domain-containing protein	-
OH217_RS17550 (OH217_17425)	aroE	3544050..3544868 (-)	819	WP_000451243.1	shikimate dehydrogenase	-
OH217_RS17555 (OH217_17430)	tsaC	3544873..3545445 (-)	573	WP_001301412.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
OH217_RS17560 (OH217_17435)	yrdD	3545450..3545992 (-)	543	WP_001129722.1	type I DNA topoisomerase	-
OH217_RS17565 (OH217_17440)	smg	3546021..3546494 (-)	474	WP_000460680.1	DUF494 family protein Smg	-
OH217_RS17570 (OH217_17445)	dprA	3546466..3547590 (-)	1125	WP_000228551.1	DNA-protecting protein DprA	Machinery gene
OH217_RS17575 (OH217_17450)	def	3547720..3548229 (+)	510	WP_000114984.1	peptide deformylase	-
OH217_RS17580 (OH217_17455)	fmt	3548244..3549191 (+)	948	WP_000004473.1	methionyl-tRNA formyltransferase	-
OH217_RS17585 (OH217_17460)	rsmB	3549237..3550526 (+)	1290	WP_000744778.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
OH217_RS17590 (OH217_17465)	trkA	3550548..3551924 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
OH217_RS17595 (OH217_17470)	mscL	3552054..3552464 (+)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40954.92 Da Isoelectric Point: 6.3122

                                >NTDB_id=643363 OH217_RS17570 WP_000228551.1 3546466..3547590(-) (dprA) [Escherichia coli strain JCL301]

MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=643363 OH217_RS17570 WP_000228551.1 3546466..3547590(-) (dprA) [Escherichia coli strain JCL301]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG

GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT

TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	P30852

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.238	98.663	0.476
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Haemophilus influenzae Rd KW20	45.104	90.107	0.406
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0643363