Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IHC89_RS01995 Genome accession   NZ_CP090495
Coordinates   403871..405562 (-) Length   563 a.a.
NCBI ID   WP_237031323.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain AS-15-0759-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 398871..410562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC89_RS01965 (IHC89_01965) mutT 399278..399685 (-) 408 WP_069530749.1 8-oxo-dGTP diphosphatase MutT -
  IHC89_RS01970 (IHC89_01970) yacG 399891..400100 (-) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  IHC89_RS01975 (IHC89_01975) zapD 400109..400846 (-) 738 WP_005297594.1 cell division protein ZapD -
  IHC89_RS01980 (IHC89_01980) coaE 400914..401516 (-) 603 WP_237031321.1 dephospho-CoA kinase -
  IHC89_RS01985 (IHC89_01985) pilD 401518..402396 (-) 879 WP_237031322.1 prepilin peptidase Machinery gene
  IHC89_RS01990 (IHC89_01990) pilC 402579..403805 (-) 1227 WP_005297600.1 type II secretion system F family protein Machinery gene
  IHC89_RS01995 (IHC89_01995) pilB 403871..405562 (-) 1692 WP_237031323.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IHC89_RS02000 (IHC89_02000) - 405565..406098 (-) 534 WP_237031324.1 pilin -
  IHC89_RS02005 (IHC89_02005) ampD 406484..407062 (+) 579 WP_005297607.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IHC89_RS02010 (IHC89_02010) pdhR 407422..408195 (+) 774 WP_237031325.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62480.10 Da        Isoelectric Point: 5.3563

>NTDB_id=642899 IHC89_RS01995 WP_237031323.1 403871..405562(-) (pilB) [Photobacterium damselae subsp. damselae strain AS-15-0759-2]
MHTTLASILHQADLISSVQQQQVEDSVSMEGISVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLAQNVDTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDDELDQTTDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQVDEIDGFMQDKLRIPTG
TTLYKANPAGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=642899 IHC89_RS01995 WP_237031323.1 403871..405562(-) (pilB) [Photobacterium damselae subsp. damselae strain AS-15-0759-2]
ATGCACACTACTCTCGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCTCGGTTCCCTCCGCACTGATCCATCTGGGCATCTTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATTTTTGGTCTCACCCTCGTTTCAGTCCATGACTACGATTTTGAAGCTTGTTGTAAAGCGGTCTATCAACGCGAT
CTGGTATTACGTCATCGCGCACTGCCCCTCGCCCAAAATGTGGACACACTGTTTTTAGGGGTCAGCGATCCATGCAATAT
CGACGGGCAAGATGAGTTCCGTTTTGCCACCGGACGTAATATTGAACCGCTACTGCTAGAAAACAAACAGCTCGAATCGG
CTATCCGTCGTATTTATGGCCGCGAGTTGGGCGAAACCACCAACATGGCCAGTCGTGATGTCAGCGATGATGATCTGAAT
AACTTAGTCGATATTGGTGATGATGAGCTGGATCAAACCACAGATCTAGGCCAAGAAAACGAACCTGTTACTCGCTATAT
CAACCAAGTACTGCTCGATGCAGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCATATGAGACCAGCTATCGGATCC
GCTTTCGTTGTGATGGCATCTTACACCAGCACGCAACACCACCAGCCCATCTTTCTCGTCGTCTTTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAGCGACGCCAACCGCAAGATGGACGCATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGATCTGCGGGTTTCTACCCTGCCCACTTTATGGGGTGAAAAAGTGGTACTGCGTATTTTAGATAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCACTGCAACAGCCGCAAGGGATG
ATCTTAATGACAGGGCCGACGGGCAGTGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCAGAAGATCCGGTAGAGATCAACTTACCCGGCATTAATCAGGTTCACATTAACAATCAAGCAGGAC
TGGGGTTTGCTGAAGCGTTGCGCTCATTTTTACGTCAAGACCCCGATGTAGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACTGCCTCTATTGCCGTTAAAGCAGCGCAAACTGGACACTTAGTGCTATCGACCTTGCACACCAATTCTGCTGCCGAAAC
CATCACCCGTTTAACCAATATGGGCATTGAAGACTTTAACCTAGCCTCATCACTGACTCTGATTATTGCTCAGCGCTTAG
GTCGTCGGCTCTGCCCTCACTGTAAACAAGTGGATGAGATTGACGGCTTTATGCAAGACAAACTGCGCATTCCGACTGGA
ACTACCTTATATAAAGCCAATCCAGCAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGCGTCGGTATCTATGAAGT
GATGCCGTTTAGTCGTCGTCTGGCTCAAGCTCTGGTTGATGGCGCGACCACCTTGGAGCTAGAAAATATCGCAGTAGAAG
AAGGGATGCTAACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGCATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

62.345

100

0.623
  pilB Vibrio parahaemolyticus RIMD 2210633

61.456

100

0.615
  pilB Vibrio campbellii strain DS40M4

60.746

100

0.607
  pilB Legionella pneumophila strain ERS1305867

50.368

96.625

0.487
  pilB Acinetobacter baylyi ADP1

49.453

97.336

0.481
  pilB Acinetobacter baumannii D1279779

50.864

92.54

0.471
  pilF Neisseria gonorrhoeae MS11

44.366

100

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

92.185

0.366