Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   N5938_RS07310 Genome accession   NZ_CP107257
Coordinates   1550240..1550863 (+) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain 2019CK-00034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1545240..1555863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5938_RS07295 (N5938_07295) - 1545927..1548398 (-) 2472 WP_019484455.1 penicillin-binding protein 1A -
  N5938_RS07300 (N5938_07300) pilM 1548583..1549647 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  N5938_RS07305 (N5938_07305) pilN 1549647..1550243 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  N5938_RS07310 (N5938_07310) pilO 1550240..1550863 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  N5938_RS07315 (N5938_07315) comL/pilP 1550860..1551384 (+) 525 WP_109223753.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  N5938_RS07320 (N5938_07320) pilQ 1551438..1553576 (+) 2139 WP_263901872.1 type 4a pilus secretin PilQ Machinery gene
  N5938_RS07325 (N5938_07325) aroK 1553588..1554106 (+) 519 WP_003095833.1 shikimate kinase AroK -
  N5938_RS07330 (N5938_07330) aroB 1554156..1555262 (+) 1107 WP_009314395.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=642723 N5938_RS07310 WP_003116135.1 1550240..1550863(+) (pilO) [Pseudomonas aeruginosa strain 2019CK-00034]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=642723 N5938_RS07310 WP_003116135.1 1550240..1550863(+) (pilO) [Pseudomonas aeruginosa strain 2019CK-00034]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396