Detailed information NTG0641741

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LW136_RS19470
• Genome accession: NZ_CP090366
• Coordinates: 4081298..4082362 (-)
• Length: 354 a.a.
• NCBI ID: WP_023446117.1
• Uniprot ID: V4QEI0
• Organism: Stutzerimonas kunmingensis strain 7850S
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4076298..4087362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LW136_RS19445 (LW136_19445)	aroK	4076865..4077410 (-)	546	WP_014821719.1	shikimate kinase AroK	-
LW136_RS19450 (LW136_19450)	pilQ	4077414..4079528 (-)	2115	WP_234173710.1	type IV pilus secretin PilQ	Machinery gene
LW136_RS19455 (LW136_19455)	pilP	4079582..4080109 (-)	528	WP_003301042.1	type 4a pilus biogenesis lipoprotein PilP	-
LW136_RS19460 (LW136_19460)	pilO	4080106..4080729 (-)	624	WP_046618307.1	type 4a pilus biogenesis protein PilO	-
LW136_RS19465 (LW136_19465)	pilN	4080726..4081298 (-)	573	WP_014821721.1	type 4a pilus biogenesis protein PilN	-
LW136_RS19470 (LW136_19470)	pilM	4081298..4082362 (-)	1065	WP_023446117.1	pilus assembly protein PilM	Machinery gene
LW136_RS19475 (LW136_19475)	-	4082543..4084975 (+)	2433	WP_234173712.1	penicillin-binding protein 1A	-
LW136_RS19480 (LW136_19480)	-	4085039..4086307 (-)	1269	WP_014821724.1	malic enzyme-like NAD(P)-binding protein	-
LW136_RS19485 (LW136_19485)	-	4086457..4087251 (-)	795	WP_234173715.1	thermonuclease family protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37671.17 Da
• Isoelectric Point: 4.6880

>NTDB_id=641741 LW136_RS19470 WP_023446117.1 4081298..4082362(-) (pilM) [Stutzerimonas kunmingensis strain 7850S]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYRVEAYAVEPLPANAVVEKNIAELEGVGQALQRVVAKAKTGCKSAVV
AVSGSAVITKIIEMDAGLSDEELENQLKIEADQYIPYPLEEVAIDFEVQGPAPRSPGRAEVLLAACRKENVEIREAALAL
AGLTAKVVDVEAYALERSYGLLAPQLGAAHGELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYDSEVLLPFKEAVVQQVSRSLQFFFAAGQFHDVDCILLAGGTASIPGLDHMIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=641741 LW136_RS19470 WP_023446117.1 4081298..4082362(-) (pilM) [Stutzerimonas kunmingensis strain 7850S]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACGCTGCTTGGGATCGATATCAGTTCGACCTCAGTCAAGCTCCTCGAATT
AAGTCGATCAGGTAGTCGCTACCGCGTCGAGGCGTATGCTGTCGAGCCGCTCCCGGCAAACGCCGTAGTTGAAAAGAACA
TCGCCGAGCTCGAGGGCGTCGGCCAGGCATTGCAGCGGGTGGTTGCCAAGGCCAAGACCGGATGCAAGTCGGCGGTAGTG
GCTGTTTCAGGCTCCGCGGTAATCACCAAGATCATCGAGATGGATGCTGGTTTGTCCGACGAAGAACTCGAGAATCAGCT
GAAGATCGAGGCGGACCAGTACATTCCCTATCCGCTGGAAGAAGTGGCGATCGACTTCGAAGTCCAGGGGCCGGCTCCTC
GCTCTCCTGGCCGAGCAGAAGTCCTGCTCGCCGCTTGCCGCAAGGAAAACGTCGAAATTCGTGAGGCAGCGCTTGCGCTG
GCGGGACTCACTGCCAAGGTGGTTGACGTCGAAGCCTATGCGCTTGAGCGGTCTTACGGGCTACTTGCTCCACAGTTGGG
TGCGGCGCATGGAGAGTTGACCGTTGCCGTGGTGGATATCGGTGCCACCATGACTACCCTTAGTGTTCTGCATAACGGTC
GTACTATTTATACCCGTGAACAGCTTTTCGGCGGGCGTCAGCTGACGGAGGAAATTCAGCGACGCTACGGCCTCTCACAA
GAAGAAGCTGGTCTAGCCAAAAAGCAAGGCGGCCTCCCAGACGACTACGACAGTGAGGTTTTGCTTCCGTTCAAAGAGGC
AGTTGTGCAGCAGGTTTCTCGCTCGTTGCAGTTCTTCTTTGCGGCAGGACAGTTCCATGATGTCGACTGCATCCTGTTAG
CGGGCGGGACGGCATCGATACCTGGGTTGGATCACATGATTCAGCAGAAGATCGGTACTCAGACTCTAGTTGCGAATCCA
TTCGCCGATATGGCTTTGAGCAGCAAAGTGAATGCTGGAGCGCTGGCTAGCGATGCGCCGGCCTTGATGATTGCTTGTGG
CCTGGCGCTGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: V4QEI0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.65	100	0.557
comM	Acinetobacter nosocomialis M2	55.65	100	0.557
comM	Acinetobacter baylyi ADP1	53.107	100	0.531
pilM	Legionella pneumophila strain ERS1305867	47.175	100	0.472