Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LN341_RS02030 Genome accession   NZ_CP090364
Coordinates   413934..415634 (-) Length   566 a.a.
NCBI ID   WP_234203943.1    Uniprot ID   -
Organism   Photobacterium sp. TLY01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408934..420634
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LN341_RS02000 (LN341_02000) mutT 409667..410065 (+) 399 WP_046220556.1 8-oxo-dGTP diphosphatase MutT -
  LN341_RS02005 (LN341_02005) yacG 410103..410306 (-) 204 WP_082095739.1 DNA gyrase inhibitor YacG -
  LN341_RS02010 (LN341_02010) zapD 410314..411078 (-) 765 WP_046220555.1 cell division protein ZapD -
  LN341_RS02015 (LN341_02015) coaE 411117..411728 (-) 612 WP_046220554.1 dephospho-CoA kinase -
  LN341_RS02020 (LN341_02020) pilD 411725..412612 (-) 888 WP_234203941.1 A24 family peptidase Machinery gene
  LN341_RS02025 (LN341_02025) - 412699..413934 (-) 1236 WP_234203942.1 type II secretion system F family protein -
  LN341_RS02030 (LN341_02030) pilB 413934..415634 (-) 1701 WP_234203943.1 GspE/PulE family protein Machinery gene
  LN341_RS02035 (LN341_02035) - 415636..416127 (-) 492 WP_046220638.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LN341_RS02040 (LN341_02040) glpT 416552..417913 (+) 1362 WP_234203944.1 glycerol-3-phosphate transporter -
  LN341_RS02045 (LN341_02045) glpQ 418009..419067 (+) 1059 WP_234204829.1 glycerophosphodiester phosphodiesterase -
  LN341_RS02050 (LN341_02050) nadC 419152..420048 (-) 897 WP_046220548.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62375.29 Da        Isoelectric Point: 6.4079

>NTDB_id=641688 LN341_RS02030 WP_234203943.1 413934..415634(-) (pilB) [Photobacterium sp. TLY01]
MNSALINTLLQSSLINPAEADRIQTGMRQHHLSDIAAVVNAGVFSDWDLAKQLAHLSALPMDDVHHYDYQAVCKRLTLRP
LIEKHSVLPLALGQDVLTLGIANPFDTRGQDECRFASGYQISPVILPLRQLENAIRRIYGEPISSTLHTASLNNNEIAQF
SAVQTAPTETTADKDDAPVSRYLNQLLLDAVRRQASDIHIEPLEKQCRIRFRCDGLLLIHATPPSDLAARLAARLKILAR
LNIAERRLPQDGRFRLPLNPELTVDMRVSTLPAQWGEKVVLRLLDSNHIPLALNRLGYTDRQRSAFEQALTKPQGLILVT
GPTGSGKTLSLYAGLSLLNQEHRNICTAEDPVEIQLPGINQLHIHPAIGLDFATALRTFLRQDPDVIMIGEIRDQETATM
AVRAAQTGHLVLSTLHTNSAADTLNRLQHLGIAPYELSASLSLIIAQRLVRCLCPHCKRPAELTASQRDRLAIDTATSLF
AANPQGCEHCNQGYLGRTGIFELVSCTTSQANPFNETALCDFMTDQADDMSLEQAGIRLLCQGITSLSELQRVLAYDSLQ
PETECG

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=641688 LN341_RS02030 WP_234203943.1 413934..415634(-) (pilB) [Photobacterium sp. TLY01]
ATGAACAGCGCCCTGATAAACACCTTACTGCAATCCAGCCTCATCAATCCGGCTGAAGCCGATCGGATCCAGACGGGCAT
GCGCCAGCATCACCTGAGCGATATCGCTGCCGTCGTCAATGCCGGCGTATTCAGCGACTGGGATCTGGCCAAGCAGCTGG
CGCACCTCAGCGCCCTACCGATGGACGATGTTCACCATTACGATTACCAGGCTGTCTGTAAACGGCTGACCCTCAGACCG
CTGATCGAAAAGCACAGTGTGCTGCCCCTTGCCCTGGGTCAGGATGTCCTGACCTTAGGGATCGCCAATCCCTTTGATAC
CCGCGGTCAGGACGAATGCCGCTTTGCCTCCGGCTATCAGATTTCACCGGTGATTTTACCGCTCAGACAGTTGGAAAACG
CTATCAGGCGGATTTATGGCGAGCCGATTTCCAGTACGCTGCATACGGCCAGCCTCAACAATAATGAGATAGCACAGTTC
TCCGCCGTCCAGACTGCGCCGACCGAAACCACAGCCGATAAGGATGACGCGCCTGTCAGCCGTTATCTTAACCAACTGCT
GCTGGACGCGGTTCGGCGCCAGGCATCTGACATCCATATCGAACCTTTAGAAAAACAGTGCCGGATCCGATTTCGCTGCG
ATGGCCTGCTGTTGATTCATGCCACACCGCCTTCGGATTTGGCAGCCCGTCTGGCCGCCAGGCTCAAAATACTCGCCCGA
CTCAACATAGCTGAGCGCCGCCTGCCCCAGGATGGCCGCTTCAGACTGCCGCTCAACCCAGAGCTGACCGTTGATATGCG
GGTGTCCACACTGCCTGCGCAGTGGGGAGAAAAAGTCGTTTTACGGTTACTCGACAGCAACCATATTCCGCTGGCGCTCA
ATCGGCTGGGCTATACCGACAGGCAGCGCAGTGCCTTTGAGCAGGCGCTGACCAAACCACAAGGTCTGATCCTGGTGACC
GGCCCGACCGGCAGCGGAAAAACCCTGTCGCTGTATGCCGGCCTGAGTTTGCTCAACCAGGAGCACCGCAACATTTGTAC
CGCAGAGGATCCGGTCGAAATTCAACTTCCCGGGATCAATCAGCTCCACATTCACCCGGCGATAGGGCTGGATTTTGCCA
CGGCACTGCGCACTTTTCTGCGCCAGGATCCCGACGTGATCATGATAGGCGAGATCCGGGATCAGGAAACCGCAACCATG
GCGGTACGCGCGGCACAAACAGGACACCTGGTCCTGTCGACTTTGCACACCAACTCCGCCGCCGATACCCTGAACCGGTT
ACAACATCTGGGGATCGCGCCCTATGAGCTCAGTGCCTCCCTCAGCCTGATTATCGCCCAGCGGCTGGTCCGCTGCCTGT
GCCCGCACTGTAAGCGGCCGGCTGAGTTAACGGCCAGTCAGCGCGACAGACTGGCGATAGACACCGCAACATCCTTGTTC
GCCGCCAACCCTCAGGGGTGCGAGCACTGTAATCAGGGCTACTTAGGCAGAACCGGGATATTTGAACTGGTCTCCTGCAC
AACCAGTCAGGCAAACCCGTTCAATGAAACCGCGCTCTGCGATTTTATGACTGATCAGGCTGACGACATGTCGCTGGAGC
AAGCTGGGATCCGCCTGCTCTGCCAGGGGATCACCAGCCTGAGCGAGCTGCAACGGGTGCTGGCGTATGACAGTCTGCAG
CCCGAGACTGAGTGCGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)

Type II/IV secretion system protein (T2SSE)

(185-451)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

48.673

99.823

0.486
  pilB Vibrio cholerae strain A1552

48.319

99.823

0.482
  pilB Vibrio campbellii strain DS40M4

47.687

99.293

0.473
  pilB Legionella pneumophila strain ERS1305867

40.82

99.117

0.405
  pilB Acinetobacter baylyi ADP1

40.459

100

0.405
  pilB Acinetobacter baumannii D1279779

43.133

91.343

0.394
  pilF Neisseria gonorrhoeae MS11

37.762

100

0.382